Following the Mw 6.3 L’Aquila Earthquake of 6^th April, 2009, the Colle di Roio village, central Italy, suffered severe building damages. The village is located on top of an elongated carbonate ridge characterized by a complex subsurface structure, a condition prone to seismic amplification due to topographic and stratigraphic effects. We address the role of the subsurface structure and topography in the ground-motion amplification observed at the ridge top. To characterize the subsurface structure of the ridge we performed geological investigations and ambient vibration measurements in single-station as well as 2D-array configuration. Geological investigations pointed out that the ridge top is characterized by the presence of fractured rock material as a consequence of its anticlinal fold structure. Horizontal-to-vertical spectral ratio processing of ambient vibration records (HVSR) showed a broad peak in the HVSR functions in the frequency range 4-6 Hz and 2D-array data demonstrated that locally the subsurface structure at the ridge top cannot be considered homogeneous. In summer 2009, we further installed one accelerometric station on the ridge top to experimentally evaluate the site amplification. By means of Horizontal-to-Vertical spectral ratio analysis of a sample of 18 weak-motion records (H/V), we found that ground-motion amplification occurs in a narrow frequency range centred around 4 Hz with mean ratio amplitude of 6. We also analysed the dependence of seismic amplification on the azimuth by calculating H/V ratios for horizontal components rotated into a range of azimuths. This analysis showed that the higher level of horizontal amplification occurs in the direction perpendicular to the ridge trending direction. With the aim of evaluating the contribution of the topography and the local subsurface structure on the observed seismic amplification, we performed 2D finite difference modelling of wave propagation through the ridge, adopting both homogeneous and heterogeneous models. We were able to reproduce the frequency dependent amplification only by using an heterogeneous 2D model with a low velocity layer (shear-wave velocity of 400 m/s and maximum thickness of 20 m) at the ridge top. From the comparison between observations and numerical results we suggest that the amplification at the Colle di Roio ridge top is due to the interplay between local shear-wave velocity structure and topography, rather than to topography alone.