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Light is a crucial element for plant growth and production. High-pressure sodium (HPS) lamps are considered not very electrically efficient as they generate high radiant heat, and as a consequence, there has been a lot of interest in replacing HPS lamps with new more efficient lighting sources in the form of light-emitting diodes (LEDs). LEDs have a linear photon output with the electrical input current, and this great feature allows the design of lighting arrays that match the plant’s needs. In the current study, light spectrum absorbance of pigments extracted from 14 plant species was analyzed. Two absorbance peaks were observed in the Photosynthetically Active Radiation (PAR) region: one at 435 nm and the other at 665 nm. The light spectrum array was designed to produce the spectrum absorbed by basil pigments. This included the use of new wavelengths of 435 ± 5 nm to cover the blue region. Moreover, the ratio between blue and red was considered to match the absorbance of basil pigment. The use of a light spectrum that matches the plant absorbance significantly improved the investigated physiological parameters and increased the growth yield of basil. Moreover, this is the first to confirm the great positive impact of using 435 nm light spectrum in comparison with the commercially widely used 450 nm LED spectrum. This investigation has great scientific and commercial applications in the field of indoor faming and plant factory systems.
Hail Z. Rihan; Mohammed Aldarkazali; Shiren J. Mohamed; Nancy B. McMulkin; Marwa H. Jbara; Michael P. Fuller. A Novel New Light Recipe Significantly Increases the Growth and Yield of Sweet Basil (Ocimum basilicum) Grown in Plant Factory System. Agronomy 2020, 10, 934 .
AMA StyleHail Z. Rihan, Mohammed Aldarkazali, Shiren J. Mohamed, Nancy B. McMulkin, Marwa H. Jbara, Michael P. Fuller. A Novel New Light Recipe Significantly Increases the Growth and Yield of Sweet Basil (Ocimum basilicum) Grown in Plant Factory System. Agronomy. 2020; 10 (7):934.
Chicago/Turabian StyleHail Z. Rihan; Mohammed Aldarkazali; Shiren J. Mohamed; Nancy B. McMulkin; Marwa H. Jbara; Michael P. Fuller. 2020. "A Novel New Light Recipe Significantly Increases the Growth and Yield of Sweet Basil (Ocimum basilicum) Grown in Plant Factory System." Agronomy 10, no. 7: 934.
Light is a crucial element for plant growth and production. In areas where natural light is not sufficient for optimal plant growth and production, high pressure sodium (HPS) light sources are widely used. However, HPS lamps are considered not very electrically efficient generating high radiant heat and as a consequence, there has been a lot of interest in replacing HPS lamps with new more efficient lighting sources in the form of light-emitting diodes (LEDs). The effects of three lighting sources (White LED, Blue/Red LED and HPS) on the growth, development and on the essential oil yield and quality of sweet basil and bush basil were investigated. There was a clear advantage to the Blue/Red (452 nm and 632 nm, respectively) LED on virtually all growth and physiological parameters measured for both basil species. The HPS lighting system always performed least effectively in all comparisons. Combining increases in plant yield and increases in oil yield the Blue/Red LED array outperformed the HPS lights by a factor of approximately double, with the white LED being intermediate between these two extremes.
Mohammed Aldarkazali; Hail Z. Rihan; Demelza Carne; Michael P. Fuller. The Growth and Development of Sweet Basil (Ocimum basilicum) and Bush Basil (Ocimum minimum) Grown under Three Light Regimes in a Controlled Environment. Agronomy 2019, 9, 743 .
AMA StyleMohammed Aldarkazali, Hail Z. Rihan, Demelza Carne, Michael P. Fuller. The Growth and Development of Sweet Basil (Ocimum basilicum) and Bush Basil (Ocimum minimum) Grown under Three Light Regimes in a Controlled Environment. Agronomy. 2019; 9 (11):743.
Chicago/Turabian StyleMohammed Aldarkazali; Hail Z. Rihan; Demelza Carne; Michael P. Fuller. 2019. "The Growth and Development of Sweet Basil (Ocimum basilicum) and Bush Basil (Ocimum minimum) Grown under Three Light Regimes in a Controlled Environment." Agronomy 9, no. 11: 743.
A. Mohamed; J. Shaw-Sutton; B.M. Green; W Andrews; E.J. Rolley-Parnell; Y. Zhou; P. Zhou; X. Mao; Michael Fuller; M.F. Stoelen. Soft manipulator robot for selective tomato harvesting. Precision agriculture ’19 2019, 1 .
AMA StyleA. Mohamed, J. Shaw-Sutton, B.M. Green, W Andrews, E.J. Rolley-Parnell, Y. Zhou, P. Zhou, X. Mao, Michael Fuller, M.F. Stoelen. Soft manipulator robot for selective tomato harvesting. Precision agriculture ’19. 2019; ():1.
Chicago/Turabian StyleA. Mohamed; J. Shaw-Sutton; B.M. Green; W Andrews; E.J. Rolley-Parnell; Y. Zhou; P. Zhou; X. Mao; Michael Fuller; M.F. Stoelen. 2019. "Soft manipulator robot for selective tomato harvesting." Precision agriculture ’19 , no. : 1.
F.B. Klein; A. Wilmot; V.F. de Tejada; B.L. Rodriguez; I. Requena; S. Busch; A. Rondepierre; T. Auzeeri; T. Sauerwald; W.F.P. Andrews; H. Rihan; M.P. Fuller; M.F. Stoelen. Proof-of-concept modular robot platform for cauliflower harvesting. Precision agriculture ’19 2019, 1 .
AMA StyleF.B. Klein, A. Wilmot, V.F. de Tejada, B.L. Rodriguez, I. Requena, S. Busch, A. Rondepierre, T. Auzeeri, T. Sauerwald, W.F.P. Andrews, H. Rihan, M.P. Fuller, M.F. Stoelen. Proof-of-concept modular robot platform for cauliflower harvesting. Precision agriculture ’19. 2019; ():1.
Chicago/Turabian StyleF.B. Klein; A. Wilmot; V.F. de Tejada; B.L. Rodriguez; I. Requena; S. Busch; A. Rondepierre; T. Auzeeri; T. Sauerwald; W.F.P. Andrews; H. Rihan; M.P. Fuller; M.F. Stoelen. 2019. "Proof-of-concept modular robot platform for cauliflower harvesting." Precision agriculture ’19 , no. : 1.
Drought is a major limiting factor of wheat production globally. In this research, salicylic acid was used in order to increase drought tolerance in Tamooz 2 of Iraqi wheat. It was observed that the SA treatment had animportant positive impact on the final wheat biomass and on the yield components (spike drying weight, grain dry weight, 1000 grain dry weight). The significant positive role of SA treatment on the up-regulation of CBF14 gene was confirmed. The expression of CBF14 was significantly affected by the developmental stage of wheat plants and the period between SA spray and sampling. We believe that results obtained in this study help to understand more deeply the molecular mechanism of drought tolerance in wheat. It could also have important practical applications, especially for wheat growers in dry climates.
Fakhriya Kareem; Hail Rihan; Michael Fuller. The Effect of Exogenous Applications of Salicylic Acid on Drought Tolerance and Up-Regulation of the Drought Response Regulon of Iraqi Wheat. Journal of Crop Science and Biotechnology 2019, 22, 37 -45.
AMA StyleFakhriya Kareem, Hail Rihan, Michael Fuller. The Effect of Exogenous Applications of Salicylic Acid on Drought Tolerance and Up-Regulation of the Drought Response Regulon of Iraqi Wheat. Journal of Crop Science and Biotechnology. 2019; 22 (1):37-45.
Chicago/Turabian StyleFakhriya Kareem; Hail Rihan; Michael Fuller. 2019. "The Effect of Exogenous Applications of Salicylic Acid on Drought Tolerance and Up-Regulation of the Drought Response Regulon of Iraqi Wheat." Journal of Crop Science and Biotechnology 22, no. 1: 37-45.
Viral infection is one of the most serious biotic stresses, which disturbs the growth and productivity of many horticultural crops, including that of fig (Ficus carica L.). The production of plants free of viruses, such as fig mosaic virus (FMV), has become a priority in many plant breeding programs. In this study, leaves from plants of two fig cultivars, Kodato and Dattora, infected with FMV were collected from both Mecca and Al-Taif, Saudi Arabia. Transmission electron microscopy of ultrathin leaf sections showed double membrane bodies, characteristic of FMV particles, only in the mesophyll cells of infected samples. Protein analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of a protein band with a molecular weight of 35 kDa, which corresponded to the viral coat protein; and FMV was confirmed by Western blot and enzyme-linked immunosorbent assay (ELISA) tests. To obtain virus-free plants, apical shoot culture was applied. A comparison of various artificial media with different concentrations of growth regulators was evaluated to optimize shoot formation, shoot multiplication, and root formation, and was followed by plant acclimation ex vitro. Direct ELISA analysis of shoots micropropagated from meristem tip explants indicated that there were virus-free shoots, when compared to infected plants (positive control), while there were no significant differences between these explants and healthy samples (negative control). This study demonstrated that in vitro micropropagation of Saudi F. carica infected with FMV virus led to the successful elimination of the virus.
Hassan S. M. Al-Zahrani; Omar A. Almaghrabi; Michael Fuller; Hemaid I. A. Soliman; Muhammad Farooq; Ehab M. R. Metwali. Micropropagation of virus-free plants of Saudi fig (Ficus carica L.) and their identification through enzyme-linked immunosorbent assay methods. In Vitro Cellular & Developmental Biology - Animal 2018, 54, 626 -636.
AMA StyleHassan S. M. Al-Zahrani, Omar A. Almaghrabi, Michael Fuller, Hemaid I. A. Soliman, Muhammad Farooq, Ehab M. R. Metwali. Micropropagation of virus-free plants of Saudi fig (Ficus carica L.) and their identification through enzyme-linked immunosorbent assay methods. In Vitro Cellular & Developmental Biology - Animal. 2018; 54 (6):626-636.
Chicago/Turabian StyleHassan S. M. Al-Zahrani; Omar A. Almaghrabi; Michael Fuller; Hemaid I. A. Soliman; Muhammad Farooq; Ehab M. R. Metwali. 2018. "Micropropagation of virus-free plants of Saudi fig (Ficus carica L.) and their identification through enzyme-linked immunosorbent assay methods." In Vitro Cellular & Developmental Biology - Animal 54, no. 6: 626-636.
Agriculture open access journals
Fuller Mp. Exogenously Applied Antioxidants and Biostimulants Counteract the Adverse Effect of Biotic Stress in Wheat Plant. Agricultural Research & Technology: Open Access Journal 2017, 12, 1 .
AMA StyleFuller Mp. Exogenously Applied Antioxidants and Biostimulants Counteract the Adverse Effect of Biotic Stress in Wheat Plant. Agricultural Research & Technology: Open Access Journal. 2017; 12 (4):1.
Chicago/Turabian StyleFuller Mp. 2017. "Exogenously Applied Antioxidants and Biostimulants Counteract the Adverse Effect of Biotic Stress in Wheat Plant." Agricultural Research & Technology: Open Access Journal 12, no. 4: 1.
Artificial seeds are artificially encapsulated somatic embryos (usually) or other vegetative parts such as shoot buds, cell aggregates, auxiliary buds, or any other micropropagules which can be sown as a seed and converted into a plant under in vitro or in vivo conditions. An improved artificial seed production technique is considered a valuable alternate technology of propagation in many commercially important crops and a significant method for mass propagation of elite plant genotypes. The production of plant clones multiplied by tissue culture and distributed as artificial seeds could be a useful alternative to the costly F1 hybrids for different plant crops. The delivery of artificial seeds also facilitates issues such as undertaking several ways for scaling up in vitro cultures and acclimatization to ex vitro conditions. The development of an artificial seed technique also provides a great approach for the improvement of various plant species such as trees and crops.
Hail Z. Rihan; Fakhriya Kareem; Mohammed E. El-Mahrouk; Michael P. Fuller. Artificial Seeds (Principle, Aspects and Applications). Agronomy 2017, 7, 71 .
AMA StyleHail Z. Rihan, Fakhriya Kareem, Mohammed E. El-Mahrouk, Michael P. Fuller. Artificial Seeds (Principle, Aspects and Applications). Agronomy. 2017; 7 (4):71.
Chicago/Turabian StyleHail Z. Rihan; Fakhriya Kareem; Mohammed E. El-Mahrouk; Michael P. Fuller. 2017. "Artificial Seeds (Principle, Aspects and Applications)." Agronomy 7, no. 4: 71.
Hail Z. Rihan; Mohammed Al-Issawi; Michael Fuller. Upregulation of CBF/DREB1 and cold tolerance in artificial seeds of cauliflower (Brassica oleracea var. botrytis). Scientia Horticulturae 2017, 225, 299 -309.
AMA StyleHail Z. Rihan, Mohammed Al-Issawi, Michael Fuller. Upregulation of CBF/DREB1 and cold tolerance in artificial seeds of cauliflower (Brassica oleracea var. botrytis). Scientia Horticulturae. 2017; 225 ():299-309.
Chicago/Turabian StyleHail Z. Rihan; Mohammed Al-Issawi; Michael Fuller. 2017. "Upregulation of CBF/DREB1 and cold tolerance in artificial seeds of cauliflower (Brassica oleracea var. botrytis)." Scientia Horticulturae 225, no. : 299-309.
A total of 145007 reads were obtained from pyrosequencing for all the 4 samples. The total count ranged from 11,301,014 (Mecca old road) to 23,503,512 bp (Thuwal). A total of 460 fungal species belonging to 133 genera, 58 families, 33 orders, 13 classes and 4 phyla was identified across the four sites. The most abundant phylum at all four sites was Ascomycota followed by Basidiomycota. Four phyla (Ascomycota—99.31%, Basidiomycota—0.59%, Chytridiomycota—0.04%, Glomeromycota—0.03%) were detected in Khulais. Except for Glomeromycota, all phyla were detected at Mecca old road (Ascomycota—74.26%, Basidiomycota—25.71%, Chytridiomycota—0.01%) and Thuwal (Ascomycota—99.59%, Basidiomycota—0.40%, Chytridiomycota—0.002%); while only Ascomycota—90.98% and Basidiomycota—9.01% were detected in Asfan road. At the class level, Sordariomycetes was predominantly observed at Asfan road—59.88%, Khulais—68.26% and Thuwal—94.84%; while Pezizomycetes was dominant at Mecca old road—56.01%, was absent at Asfan road. Agaricomycetes was present only at Mecca old road—25.73%; while Tremellomycetes—5.77%, Malasseizomycetes—2.13% and Microbotryomycetes—1.10% were found only at Asfan road. The phylogenetic trees revealed that clear genus level differences are visible across all the four sites, with an overall predominance of Thielavia followed by Madurella, Aspergillus, and Gelasinospora. Chaetomium sp., Aspergillus caespitosus and Aspergillus sp. were found in moderate (Mecca old road and Thuwal) to abundant (Asfan road and Khulais) quantities. Thielavia sp., Thielavia hyalocarpa and Madurella sp. are found in moderate quantities at Khulais and Mecca old road, while in abundant levels at Asfan road and Thuwal. Fusarium equisati and F. oxysporum were detected at Thuwal and Khulais. Sordaria araneosa was present at Khulais, while Malasseiza globosa species was detected in moderate quantities across all sites except Khulais.
Tarek A. A. Moussa; Hassan S. Al-Zahrani; Omar A. Almaghrabi; Tamer S. Abdelmoneim; Michael Fuller. Comparative metagenomics approaches to characterize the soil fungal communities of western coastal region, Saudi Arabia. PLOS ONE 2017, 12, e0185096 .
AMA StyleTarek A. A. Moussa, Hassan S. Al-Zahrani, Omar A. Almaghrabi, Tamer S. Abdelmoneim, Michael Fuller. Comparative metagenomics approaches to characterize the soil fungal communities of western coastal region, Saudi Arabia. PLOS ONE. 2017; 12 (9):e0185096.
Chicago/Turabian StyleTarek A. A. Moussa; Hassan S. Al-Zahrani; Omar A. Almaghrabi; Tamer S. Abdelmoneim; Michael Fuller. 2017. "Comparative metagenomics approaches to characterize the soil fungal communities of western coastal region, Saudi Arabia." PLOS ONE 12, no. 9: e0185096.
The development stages of conventional cauliflower seeds were studied and the accumulation of dehydrin proteins through the maturation stages was investigated with the aim of identifying methods to improve the viability of artificial seeds of cauliflower. While carbohydrate, ash and lipids increased throughout the development of cauliflower traditional seeds, proteins increased with the development of seed and reached the maximum level after 75 days of pollination, however, the level of protein started to decrease after that. A significant increase in the accumulation of small size dehydrin proteins (12, 17, 26 KDa) was observed during the development of cauliflower seeds. Several experiments were conducted in order to increase the accumulation of important dehydrin proteins in cauliflower microshoots (artificial seeds). Mannitol and ABA (Absisic acid) increased the accumulation of dehydrins in cauliflower microshoots while cold acclimation did not have a significant impact on the accumulation of these proteins. Molybdenum treatments had a negative impact on dehydrin accumulation. Dehydrins have an important role in the drought tolerance of seeds and, therefore, the current research helps to improve the accumulation of these proteins in cauliflower artificial seeds. This in turns improves the quality of these artificial seeds. The current results suggest that dehydrins do not play an important role in cold tolerance of cauliflower artificial seeds. This study could have an important role in improving the understanding of the molecular mechanism of abiotic stress tolerance in plants.
Hail Z. Rihan; Mohammed Al-Issawi; Michael P. Fuller. An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds. Plant Physiology and Biochemistry 2017, 116, 91 -105.
AMA StyleHail Z. Rihan, Mohammed Al-Issawi, Michael P. Fuller. An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds. Plant Physiology and Biochemistry. 2017; 116 ():91-105.
Chicago/Turabian StyleHail Z. Rihan; Mohammed Al-Issawi; Michael P. Fuller. 2017. "An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds." Plant Physiology and Biochemistry 116, no. : 91-105.
Polyethylene glycol (PEG) can be used in somatic embryogenesis to enhance embryogenic development and improve the success of in vitro culture but PEG also causes osmotic stress in developing embryos. The effects of PEG on embryo growth and development in date palm cell suspension culture and associated antioxidant enzyme activities were evaluated. Callus maintained on MS basal media was transferred to regeneration liquid media supplemented with increasing levels (0–20%) of polyethylene glycol 6000 (PEG) to induce osmotic stress. The degree of embryogenic callus formation, its fresh weight, and the percentage of normal embryo callus shapes were increased with an increase in the level of PEG up to 10%. Total soluble protein (TSP), proline, glycine betaine (GB), total soluble phenol (TSPh), total sugars (TS), and total soluble organic acids (TOA) also increased whereas superoxide dismutase (SOD) activity decreased in response to PEG supplementation. Raising the PEG level increased malondialdehyde (MDA) concentration up to 10% PEG and thereafter decreased. Glutathione reductase (GR) and catalase (CAT) activities decreased at the highest levels of PEG. The proportion of normal embryo developmental shapes were about 50% compared with 20% abnormal shapes at optimum levels of PEG. Proliferation of somatic embryos was influenced by their developmental shapes. Cv. Samani accumulated more organic solutes compared with cv. Sewi in both control and stress inducing media. In contrast, lipid peroxidation, GR, SOD, and CAT activities were significantly higher in cv. Sewi than in cv. Samani indicating that the cv. Samani had the ability to tolerate a higher level of osmotic stress compared to cv. Sewi due to the enhanced osmotic re-balancing within its tissues.
Mohamed N. Helaly; Hanan A.R. El-Hosieny; Naser M. El-Sarkassy; Michael P. Fuller. Growth, lipid peroxidation, organic solutes, and anti-oxidative enzyme content in drought-stressed date palm embryogenic callus suspension induced by polyethylene glycol. In Vitro Cellular & Developmental Biology - Animal 2017, 53, 133 -141.
AMA StyleMohamed N. Helaly, Hanan A.R. El-Hosieny, Naser M. El-Sarkassy, Michael P. Fuller. Growth, lipid peroxidation, organic solutes, and anti-oxidative enzyme content in drought-stressed date palm embryogenic callus suspension induced by polyethylene glycol. In Vitro Cellular & Developmental Biology - Animal. 2017; 53 (2):133-141.
Chicago/Turabian StyleMohamed N. Helaly; Hanan A.R. El-Hosieny; Naser M. El-Sarkassy; Michael P. Fuller. 2017. "Growth, lipid peroxidation, organic solutes, and anti-oxidative enzyme content in drought-stressed date palm embryogenic callus suspension induced by polyethylene glycol." In Vitro Cellular & Developmental Biology - Animal 53, no. 2: 133-141.
Abiotic stress is one of the main causes of crop reduction globally. Among the different abiotic stresses, cold is an essential factor that limits crop productivity worldwide. Low temperature affects the growth, development and distribution of agronomic species throughout the world. Cold stress is a serious threat to the sustainability of crop yields. Indeed, cold stress can cause major crop losses. A significant number of researches have been reviewed and discussed in this study in order to improve the understanding of the physiological and genetic nature and function of plant cold tolerance. Recent developments in determining the mechanism of genes with roles in freezing tolerance and the systems involved in low-temperature gene regulation and signal transduction are described. The roles of a family of Arabidopsis transcription factors, the CBF/DREB1 proteins, have been described and its role in controlling the expression of a regulon of cold-induced genes (COR) that increase plant freezing tolerance has been explained. Moreover, this study has reviewed the recent application applied to improve the cold tolerance of plants such as molybdenum. The use of infrared camera to study the process of plant injuries caused by low temperature has also been reviewed.
Hail Z. Rihan; Mohammed Al-Issawi; Michael Fuller. Advances in physiological and molecular aspects of plant cold tolerance. Journal of Plant Interactions 2017, 12, 143 -157.
AMA StyleHail Z. Rihan, Mohammed Al-Issawi, Michael Fuller. Advances in physiological and molecular aspects of plant cold tolerance. Journal of Plant Interactions. 2017; 12 (1):143-157.
Chicago/Turabian StyleHail Z. Rihan; Mohammed Al-Issawi; Michael Fuller. 2017. "Advances in physiological and molecular aspects of plant cold tolerance." Journal of Plant Interactions 12, no. 1: 143-157.
Cadmium (Cd) in agricultural soil negatively affects crops yield and compromises food safety. Remediation of polluted soil is necessary for the re-establishment of sustainable agriculture and to prevent hazards to human health and environmental pollution. Phytoremediation is a promising technology for decontamination of polluted soil. The present study investigated the effect of molybdenum (Mo) (0.5, 1.0 and 2.0 ppm) on endogenous production of total phenolics and free proline, plant biomass and photosynthetic pigments in Ricinus communis plants grown in Cd (25, 50 and 100 ppm) contaminated soils and the potential for Cd phytoextraction. Mo was applied via seed soaking, soil addition and foliar spray. Foliar sprays significantly increased plant biomass, Cd accumulation and bioconcentration. Phenolic concentrations showed significantly positive correlations with Cd accumulation in roots (R 2 = 0.793, 0.807 and 0.739) and leaves (R 2 = 0.707, 721 and 0.866). Similarly, proline was significantly positively correlated with Cd accumulation in roots (R 2 = 0.668, 0.694 and 0.673) and leaves (R 2 = 0.831, 0.964 and 0.930). Foliar application was found to be the most effective way to deliver Mo in terms of increase in plant growth, Cd accumulation and production of phenolics and proline.
Fazal Hadi; Nasir Ali; Michael Fuller. Molybdenum (Mo) increases endogenous phenolics, proline and photosynthetic pigments and the phytoremediation potential of the industrially important plant Ricinus communis L. for removal of cadmium from contaminated soil. Environmental Science and Pollution Research 2016, 23, 20408 -20430.
AMA StyleFazal Hadi, Nasir Ali, Michael Fuller. Molybdenum (Mo) increases endogenous phenolics, proline and photosynthetic pigments and the phytoremediation potential of the industrially important plant Ricinus communis L. for removal of cadmium from contaminated soil. Environmental Science and Pollution Research. 2016; 23 (20):20408-20430.
Chicago/Turabian StyleFazal Hadi; Nasir Ali; Michael Fuller. 2016. "Molybdenum (Mo) increases endogenous phenolics, proline and photosynthetic pigments and the phytoremediation potential of the industrially important plant Ricinus communis L. for removal of cadmium from contaminated soil." Environmental Science and Pollution Research 23, no. 20: 20408-20430.
The data presented contains the sequences of fungal Internal Transcribed Spacer (ITS) and 18S rRNA gene from a metagenome of the Mecca region, Saudi Arabia. Sequences were amplified using fungal specific primers, which amplified the amplicon aligned between the 18S and 28S rRNA genes. A total of 460 fungal species belonging to 133 genera, 58 families, 33 orders, 13 classes and 4 phyla were identified in four contrasting locations. The raw sequencing data used to perform this analysis along with FASTQ file are located in the NCBI Sequence Read Archive (SRA) under accession numbers: SRR3150823, SRR3144873, SRR3150825 and SRR3150846.
Tarek A.A. Moussa; Hassan S. Al-Zahrani; Omar A. Almaghrabi; Nevien M. Sabry; Michael Fuller. Metagenomic analysis of fungal taxa inhabiting Mecca region, Saudi Arabia. Genomics Data 2016, 9, 126 -7.
AMA StyleTarek A.A. Moussa, Hassan S. Al-Zahrani, Omar A. Almaghrabi, Nevien M. Sabry, Michael Fuller. Metagenomic analysis of fungal taxa inhabiting Mecca region, Saudi Arabia. Genomics Data. 2016; 9 ():126-7.
Chicago/Turabian StyleTarek A.A. Moussa; Hassan S. Al-Zahrani; Omar A. Almaghrabi; Nevien M. Sabry; Michael Fuller. 2016. "Metagenomic analysis of fungal taxa inhabiting Mecca region, Saudi Arabia." Genomics Data 9, no. : 126-7.
Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L−1) and ZnO-NPs (0, 15 and 30 mg L−1). Treatments with NaCl at both 3 and 6 g L−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162 kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa.
Hesham F. Alharby; Ehab M.R. Metwali; Michael P. Fuller; Amal Y. Aldhebiani. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato ( Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles. Saudi Journal of Biological Sciences 2016, 23, 773 -781.
AMA StyleHesham F. Alharby, Ehab M.R. Metwali, Michael P. Fuller, Amal Y. Aldhebiani. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato ( Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles. Saudi Journal of Biological Sciences. 2016; 23 (6):773-781.
Chicago/Turabian StyleHesham F. Alharby; Ehab M.R. Metwali; Michael P. Fuller; Amal Y. Aldhebiani. 2016. "The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato ( Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles." Saudi Journal of Biological Sciences 23, no. 6: 773-781.
The properties of nanomaterials and their potential applications have been given considerable attention by researchers in various fields, especially agricultural biotechnology. However, not much has been done to evaluate the role or effect of zinc oxide nanoparticles (ZnO-NP) in regulating physiological and biochemical processes in response to salt-induced stress. For this purpose, some callus growth traits, plant regeneration rate, mineral element (sodium, potassium, phosphorous and nitrogen) contents and changes in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in tissues of five tomato cultivars were investigated in a callus culture exposed to elevated concentrations of salt (3.0 and 6.0 g L-1NaCl), and in the presence of zinc oxide nanoparticles (15 and 30 mg L-1). The relative callus growth rate was inhibited by 3.0 g L-1 NaCl; this was increased dramatically at 6.0 g L-1. Increasing exposure to NaCl was associated with a significantly higher sodium content and SOD and GPX activities. Zinc oxide nanoparticles mitigated the effects of NaCl, and in this application of lower concentrations (15 mg L-1) was more effective than a higher concentration (30 mg L-1). This finding indicates that zinc oxide nanoparticles should be investigated further as a potential anti-stress agent in crop production. Different tomato cultivars showed different degrees of tolerance to salinity in the presence of ZnO-NP. The cultivars Edkawy, followed by Sandpoint, were less affected by salt stress than the cultivar Anna Aasa.
Hesham F. Alharby; Ehab M.R. Metwali; Michael Fuller; Amal Y. Aldhebiani. Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Archives of Biological Sciences 2016, 68, 723 -735.
AMA StyleHesham F. Alharby, Ehab M.R. Metwali, Michael Fuller, Amal Y. Aldhebiani. Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Archives of Biological Sciences. 2016; 68 (4):723-735.
Chicago/Turabian StyleHesham F. Alharby; Ehab M.R. Metwali; Michael Fuller; Amal Y. Aldhebiani. 2016. "Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress." Archives of Biological Sciences 68, no. 4: 723-735.
Under cold stress, reactive oxygen species (ROS) are considered the main source of damage to plant cells. Mechanisms of ROS scavenging in wheat are very important during stress and the antioxidant enzymes superoxide dismutase, Catalase and Glutathione peroxidase are key to facilitating ROS scavenging. Molybdenum (Mo) is involved in many plant physiological and biochemical processes including antioxidant enzymes. This study reports research to investigate the effect of Mo application in enhancing antioxidant enzymes in two wheat cultivars. The results confirmed that antioxidant defense is important in wheat that is exposed to abiotic stress and that changes in activities of antioxidant enzymes occurred during exposure of plants to low non-freezing temperatures and by adding Mo. Mo application had a positive effect on gene expression of both Cbf14 and COR15a protein expression, indicating upregulation of the stress response regulon. In addition, Mo enhanced antioxidant enzymes activity and improved frost tolerance.
Mohammed Al-Issawi; Hail Z. Rihan; Hanady Al-Shmgani; Michael Fuller. Molybdenum application enhances antioxidant enzyme activity and COR15a protein expression under cold stress in wheat. Journal of Plant Interactions 2016, 11, 5 -10.
AMA StyleMohammed Al-Issawi, Hail Z. Rihan, Hanady Al-Shmgani, Michael Fuller. Molybdenum application enhances antioxidant enzyme activity and COR15a protein expression under cold stress in wheat. Journal of Plant Interactions. 2016; 11 (1):5-10.
Chicago/Turabian StyleMohammed Al-Issawi; Hail Z. Rihan; Hanady Al-Shmgani; Michael Fuller. 2016. "Molybdenum application enhances antioxidant enzyme activity and COR15a protein expression under cold stress in wheat." Journal of Plant Interactions 11, no. 1: 5-10.
H.Z. Rihan; M. Al-Shamari; F. Al-Swedi; S. Burchett; M.P. Fuller. THE EFFECT OF SUGAR TYPE, SOURCE AND CONCENTRATION ON CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS) MICROSHOOT PRODUCTION. Acta Horticulturae 2015, 87 -94.
AMA StyleH.Z. Rihan, M. Al-Shamari, F. Al-Swedi, S. Burchett, M.P. Fuller. THE EFFECT OF SUGAR TYPE, SOURCE AND CONCENTRATION ON CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS) MICROSHOOT PRODUCTION. Acta Horticulturae. 2015; (1099):87-94.
Chicago/Turabian StyleH.Z. Rihan; M. Al-Shamari; F. Al-Swedi; S. Burchett; M.P. Fuller. 2015. "THE EFFECT OF SUGAR TYPE, SOURCE AND CONCENTRATION ON CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS) MICROSHOOT PRODUCTION." Acta Horticulturae , no. 1099: 87-94.
M. Al Shamari; H.Z. Rihan; F. Al Swedi; M.P. Fuller. THE USE OF SOMATIC EMBRYOGENESIS IN ARTIFICIAL SEEDS PRODUCTION IN CAULIFLOWER (BRASSICA OLERACEAE VAR. BOTRYTIS). Acta Horticulturae 2015, 399 -406.
AMA StyleM. Al Shamari, H.Z. Rihan, F. Al Swedi, M.P. Fuller. THE USE OF SOMATIC EMBRYOGENESIS IN ARTIFICIAL SEEDS PRODUCTION IN CAULIFLOWER (BRASSICA OLERACEAE VAR. BOTRYTIS). Acta Horticulturae. 2015; (1099):399-406.
Chicago/Turabian StyleM. Al Shamari; H.Z. Rihan; F. Al Swedi; M.P. Fuller. 2015. "THE USE OF SOMATIC EMBRYOGENESIS IN ARTIFICIAL SEEDS PRODUCTION IN CAULIFLOWER (BRASSICA OLERACEAE VAR. BOTRYTIS)." Acta Horticulturae , no. 1099: 399-406.