Lycium ruthenicum, a halophytic shrub, has been used to remediate saline soils in northwest China. However, little is known about its root-associated microbial community and how it may be affected by the plant’s growth cycle. In this study, we investigate the microbial community structure of L. ruthenicum by examining three root compartments (rhizosphere, rhizoplane, and endosphere) during four growth stages (vegetative, flowering, fruiting, and senescence). The microbial community diversity and composition were determined by Illumina MiSeq sequencing of the 16S V3–V4 and 18S ITS regions. Proteobacteria, Actinobacteria, Bacteroidetes, Planctomycetes, and Acidobacteria were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the most dominant fungal phyla. The alpha diversity of the bacterial communities was highest in the rhizosphere and decreased from the rhizosphere to the endosphere compartments; the fungal communities did not show a consistent trend. The rhizosphere, rhizoplane, and endosphere had distinct bacterial community structures among the three root compartments and from the bulk soil. Additionally, PERMANOVA indicated that the effect of rhizocompartments explained a large proportion of the total community variation. Differential and biomarker analysis not only revealed that each compartment had unique biomarkers and was enriched for specific bacteria, but also that the biomarkers changed with the plant growth cycle. Fungi were also affected by the rhizocompartment, but to a much less so than bacteria, with significant differences in the community composition along the root compartments observed only during the vegetative and flowering stages. Instead, the growth stages appear to account for most of the fungal community variation as demonstrated by PCoA and NMDS, and supported by differential and biomarker analysis, which revealed that the fungal community composition in the rhizosphere and endosphere were dynamic in response to the growth stage. Many enriched OTUs or biomarkers that were identified in the root compartments were potentially beneficial to the plant, meanwhile, some harmful OTUs were excluded from the root, implying that the host plant can select for beneficial bacteria and fungi, which can promote plant growth or increase salt tolerance. In conclusion, the root compartment and growth stage were both determinant factors in structuring the microbial communities of L. ruthenicum, but the effects were different in bacteria and fungi, suggesting that bacterial and fungal community structures respond differently to these growth factors.

Salt stress is a key environmental factor that has adverse effects on plant growth and development. High salinity induces a series of structural and functional changes in the morphological and anatomical features. The physiological and biochemical changes in K. foliatum in response to salt stress in natural environments are still unclear. Based on this, this study compared and analyzed the differences in the physiological and biochemical indicators between the leaf and root tissues in high-salt and low-salt habitats, selecting K. foliatum as the research object. The results showed that the chlorophyll contents in the leaves of K. foliatum decreased in the high-salt habitat, while the thicknesses of the upper and lower epidermises, as well as the thicknesses of the palisade tissue, significantly increased. The high-salt environment led to decreases in the N and P contents in the leaves and root tissues of K. foliatum, resulting in changes in the stoichiometric ratio of elements. The concentrations of C, N, and P in the roots of K. foliatum were lower than those in the leaves. The accumulation of Na⁺ in the K. foliatum roots was greater than that in the leaves, and the roots could promote the transport of sodium ions to the leaves. The contents of starch and soluble sugar in the leaves showed higher proportions in the high-salt habitat than in the low-salt habitat, while the changes in the roots and leaves were the opposite. As the salt content increased, the proline contents in the leaves and roots of K. foliatum significantly increased, and the proline contents in the roots of K. foliatum were lower than those in the leaves. The leaves and roots exhibited higher levels of peroxidase and superoxide enzymes in the high-salinity habitat than in the low-salinity habitat. The superoxide dismutase (SOD) activity of the K. foliatum leaves and catalase (CAT) activity of the roots were the “central traits” in the high-salt habitat. In the low-salt habitat, the leaf malondialdehyde (MDA) and root C/N were the central traits of the leaves and roots, indicating that K. foliatum adapts to changes in salt environments in different ways.

In order to study the characteristics and sources of heavy metal pollution within different grassland soil categories in Altay, 147 soil samples were collected in Fuyun County according to grassland type, and the contents of seven heavy metals (Cu, Pb, Cr, Zn, As, Ni, and Cd) were determined. The heavy metal pollution in the soil was evaluated using the Nemerow pollution and geological accumulation index methods. The absolute principal component score–multiple linear regression model (APCS-MLR) model was used to analyze the sources of heavy metals and the contribution rate of the pollution sources. The results showed that (1) the heavy metal content of 0~10 cm soil in the mining area was generally higher than the remaining two layers. Natural grassland was the most seriously contaminated, and the order of its contamination was Zn > Pb > Ni > Cd > Cu > Cr > As. (2) The evaluation results of the comprehensive pollution index showed that Ni and Cd were the main pollutants in five grassland types, and the evaluation results of the geological accumulation index method showed that natural grasslands were more polluted. (3) Three pollution sources were obtained according to the analysis using the APCS-MLR model, including natural and mining activity sources (32.6%), industrial coal combustion and sewage sources (24.7%), and an unknown source (42.7%). The results of the study can provide a basis for pollution control and ecological protection in the grasslands of Altay.

Desert oases are vital for maintaining the ecological balance in arid regions’ inland river basins. However, fine-grained assessments of water stress in desert oasis ecosystems are limited. In our study, we aimed to evaluate the water stress in desert oasis ecosystems in the middle reaches of the Keriya River Basin, with a specific focus on their ecological functions and optimizing water resource management. We hypothesized that evapotranspiration has significant effects on ecological water consumption. First, we estimated the actual evapotranspiration (ET) and potential evapotranspiration (PET) based on the SEBS (surface energy balance system) model and remote sensing downscaling model. Then, the ecological water requirement (EWR) and ecological water stress (EWS) index were constructed to evaluate the ecological water resource utilization. Finally, we explored the influencing factors and proposed coping strategies. It was found that regions with higher ET values were mainly concentrated along the Keriya River and its adjacent farmland areas, while the lower values were observed in bare land or grassland areas. The total EWR exhibited the sequence of grassland > cropland > forest, while the EWR per unit area followed the opposite order. The grassland’s EWS showed a distinct seasonal response, with severe, moderate, and mild water shortages and water plenitude corresponding to spring, summer, autumn, and winter, respectively. In contrast, the land use types with the lowest EWS were water areas that remained in a state of water plentitude grade (0.08–0.20) throughout the year. Temperature and vegetation index were identified as the primary influencing factors. Overall, this study provides a reliable method for evaluating the EWR and EWS values of basin scale vegetation, which can serve as a scientific basis for formulating water resource management and regulation policies in the region.

Currently, the ecological problems caused by the greenhouse effect are growing more serious, and implementing carbon sequestration methods is an effective way to address them. Arid and semi-arid desert areas have tremendous potential as carbon sinks, and artificial forests in these areas play an important role in absorbing and sequestering carbon dioxide. This study selected three main species of artificial protective trees along the Tarim Desert Highway—Haloxylon ammodendron (C.A.Mey.) Bunge, Calligonum mongolicum Turcz. and Tamarix chinensis Lour.—and evaluated them for their carbon sequestration, oxygen release capacity, and economic benefits using Pn (net photosynthetic rate) and biomass methods. The results showed that the average daily Pn value and carbon sequestration and oxygen release per unit leaf area of T. chinensis were significantly higher than those of H. ammodendron and C. mongolicum (p < 0.05). The total carbon storage of the three shelterbelts was 15.41 × 10⁴ t, and the carbon storage of H. ammodendron was significantly higher than that of C. mongolicum and T. chinensis (p < 0.05). According to the net photosynthetic rate method, the annual carbon sequestration and oxygen release of the shelter forest is 6.13 × 10⁴ t a⁻¹, and the transaction price is CNY 13.73 million a⁻¹. The total amount of carbon sequestration and oxygen release of the shelter forest obtained via the biomass method is 97.61 × 10⁴ t, and the transaction price is CNY 218.77 million. This study conducted research on the carbon sequestration capacity of protective forests along the Tarim Desert Highway located in an extremely arid region. It highlights the significant contribution of these protective forests in terms of carbon storage, playing a crucial role in promoting ecological restoration and sustainable development in arid areas. Additionally, this study provides a scientific basis for estimating carbon storage and promoting the sustainable management of artificial forests in arid desert regions.

The conventional reservoir operation strategy considering hydropower production ignores the ecology of the downstream rivers and causes a series of environmental problems. To ensure the sustainable development of a reservoir, the operation strategy should consider both the economic benefits of the power station and the ecological benefits in downstream rivers, the key to which is to select suitable parameters for quantifying the ecological objectives of the rivers and incorporate them into the reservoir operation model. To this end, the ecological index (EI) based on the Indicators of Hydrological Alteration (IHA) was developed to reflect the ecology of the downstream rivers, and a reservoir ecological operation model that takes into account the power generation capacity of the power station and the degree of hydrological alteration was constructed, which can be solved using the Non-Dominated Sorting Genetic Algorithm II (NSGA-II). The results show that the mean EI values increase a lot, from 0.24 to 0.62, after the construction of reservoir, and the optimal reservoir operation strategy in each typical year can reduce the hydrological alteration significantly (the reduction rates of both the abundant and dry water years exceed 20%), while ensuring the hydropower production of the power station (the reduction rates of hydropower production are just 1.64%, 3.15%, and 3.16% for abundant, normal, and dry water years, respectively), which provides a good reference for restoring the natural hydrological situation of downstream rivers.