Extended Reality (XR) applications are considered useful for skill acquisition in dental education. In this study, we examined the functionality and usefulness of an application called “SR View for Endo” that measures root canal length using a Spatial Reality Display (SRD) capable of naked-eye stereoscopic viewing. Three-dimensional computer graphics (3DCG) data of dental models were obtained and output to both the SRD and conventional 2D display devices. Forty dentists working at the Kanagawa Dental University Hospital measured root canal length using both types of devices and provided feedback through a questionnaire. Statistical analysis using one-way analysis of variance evaluated the measurement values and time, while multivariate analysis assessed the relationship between questionnaire responses and measurement time. There was no significant difference in the measurement values between the 2D device and SRD, but there was a significant difference in measurement time. Furthermore, a negative correlation was observed between the frequency of device usage and the extended measurement time of the 2D device. Measurements using the SRD demonstrated higher accuracy and shorter measurement times compared to the 2D device, increasing expectations for clinical practice in dental education and clinical education for clinical applications. However, a certain percentage of participants experienced symptoms resembling motion sickness associated with virtual reality (VR).