Simple sequence repeats (SSRs) are highly versatile markers in genetic diversity analysis and plant breeding, making them widely applicable. They hold potential in lentil (Lens culinaris) breeding for genetic diversity analysis, marker-assisted selection (MAS), and linkage mapping. However, the availability and diversity of SSR markers in lentil is limited. We used next-generation sequencing (NGS) technology to develop SSR markers in lentil. NGS allowed us to identify regions of the lentil genome that contained SSRs. Illumina Hiseq-2000 sequencing of the lentil genotype “Karacadağ” resulted in 1,727,734 sequence reads comprising more than 48,390 Mb, and contigs were mined for SSRs, resulting in the identification of a total of 8697 SSR motifs. Among these, dinucleotide repeats were the most abundant (53.38%), followed by trinucleotides (30.38%), hexanucleotides (6.96%), tetranucleotides (6.59%), and pentanucleotides (3.19%). The most frequent repeat in dinucleotides was the TC (21.80%), followed by the GA (17.60%). A total of 2000 primer pairs were designed from these motifs, and 458 SSR markers were validated following their amplified PCR products. A linkage map was constructed using these new SSRs with high linkage disequilibrium (209) and previously known SSRs (11). The highest number of SSR markers (43) was obtained in LG2, while the lowest number of SSR markers (19) was obtained in LG7. The longest linkage group (LG) was LG2 (86.84 cM), whereas the shortest linkage group was LG7 (53.46 cM). The average length between markers ranged from 1.86 cM in LG1 to 2.81 cM in LG7, and the map density was 2.16 cM. The developed SSRs and created linkage map may provide useful information and offer a new library for genetic diversity analyses, linkage mapping studies, and lentil breeding programs.

Genotypes with longer coleoptiles can be sown in deep soil layers to reach the underground moisture needed for germination in dry areas. Developing new varieties with longer coleoptiles and shorter plant heights would be novel for wheat breeding and production. In this study, coleoptile lengths of a panel of durum wheat (Triticum turgidum ssp. durum) genotypes were determined, and 14,255 DArTseq (SNP and Silico‐DArT) markers were used to identify coleoptile length‐associated markers by genome‐wide association study (GWAS). A wide genetic variation was accounted for both coleoptile length and plant height. The genetic relationships between coleoptile length and plant height were evaluated using plant height values from five different environments. Two environmentally stable MTAs were identified, one for coleoptile length (QCol.su.4BS) and one for plant height (QPh.su.4BS). These MTAs were located on the short arm of chromosome 4B, with LOD scores up to 12.00 and 17.00, respectively. A relatively high LD (r² = 0.71) was accounted for between QCol.su.4BS and QPh.su.4BS. The LD block intervals of the MTAs overlapped with some genes with roles in plant growth and development. The functions of plausible candidate genes tell us that QCol.su.4BS may be controlling coleoptile length, whereas QPh.su.4BS may be regulating plant height. The combination of the two loci would be desirable. In conclusion, this study sheds light on the genetic control of coleoptile length and its relationship with plant height in durum wheat.

This study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations of universal rice primers (URPs). A total number of 37 URP marker pairs were tested and twenty of those provided amplicons in the common vetch genome. The pairs of amplified URP markers provided a total of 83 bands and 62 of them were determined as polymorphic and were scattered to the whole genome. The average polymorphism rate of the primers was calculated as 73.5% while the polymorphism information content (PIC) values have ranged from 0.11 to 0.47 with an average of 0.24. The phylogenetic tree constructed based on UPGMA analysis provided three main clades. Two-dimensional plot of PCA and the UPGMA analysis showed that the URP markers successfully distinguished the genetic material based on their genetic origin. In conclusion, this study revealed that the use of pairwise combinations of URP markers could have a better power to reveal the level of polymorphism in plant genome.

Önemli bir kültür bitkisi olan mercimek, iklim değişikliği kaynaklı abiyotik ve biyotik streslere karşı dayanıklı, verimli ve sürdürülebilir çeşitlere ihtiyaç duymaktadır. Bu bağlamda, yabani mercimek gen kaynaklarından elde edilen genetik materyaller, genetik iyileştirme ve stres toleransı kazandırma çalışmalarında önemli bir rol oynamaktadır. Genetik çeşitlilik çalışmaları, genetik haritalama ve ileri düzey yüksek verimli dizileme teknolojileri sayesinde, streslere uyum sağlayan adaptif genler, QTL'ler ve diğer faydalı bitki özellikleri tanımlanmaktadır. Genomik teknolojilerin bitki ıslahıyla entegrasyonu, yoğun genetik bağlantı haritaları, genotipleme çalışmaları ve QTL analizleri sayesinde mercimek genomik araştırmaları büyük ilerleme kaydetmektedir. Bu derlemede, mercimekte genetik çeşitlilik, genetik haritaların oluşturulması, QTL analizleri ve genomik çalışmalar konusundaki son gelişmeler literatüre dayalı olarak tartışılmıştır.