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Plant growth-promoting bacteria (PGPB) have great potential to provide economical and sustainable solutions to current agricultural challenges. The Methylobacteria which are frequently present in the phyllosphere can promote plant growth and development. The Methylobacterium genus is composed mostly of pink-pigmented facultative methylotrophic bacteria, utilizing organic one-carbon compounds as the sole carbon and energy source for growth. Methylobacterium spp. have been isolated from diverse environments, especially from the surface of plants, because they can oxidize and assimilate methanol released by plant leaves as a byproduct of pectin formation during cell wall synthesis. Members of the Methylobacterium genus are good candidates as PGPB due to their positive impact on plant health and growth; they provide nutrients to plants, modulate phytohormone levels, and protect plants against pathogens. In this paper, interactions between Methylobacterium spp. and plants and how the bacteria promote crop growth is reviewed. Moreover, the following examples of how to engineer microbiomes of plants using plant-growth-promoting Methylobacterium are discussed in the present review: introducing external Methylobacterium spp. to plants, introducing functional genes or clusters to resident Methylobacterium spp. of crops, and enhancing the abilities of Methylobacterium spp. to promote plant growth by random mutation, acclimation, and engineering.
Cong Zhang; Meng-Ying Wang; Naeem Khan; Ling-Ling Tan; Song Yang. Potentials, Utilization, and Bioengineering of Plant Growth-Promoting Methylobacterium for Sustainable Agriculture. Sustainability 2021, 13, 3941 .
AMA StyleCong Zhang, Meng-Ying Wang, Naeem Khan, Ling-Ling Tan, Song Yang. Potentials, Utilization, and Bioengineering of Plant Growth-Promoting Methylobacterium for Sustainable Agriculture. Sustainability. 2021; 13 (7):3941.
Chicago/Turabian StyleCong Zhang; Meng-Ying Wang; Naeem Khan; Ling-Ling Tan; Song Yang. 2021. "Potentials, Utilization, and Bioengineering of Plant Growth-Promoting Methylobacterium for Sustainable Agriculture." Sustainability 13, no. 7: 3941.
Macrofungi, which are also known as mushrooms, can produce various bioactive constituents and have become promising resources as lead drugs and foods rich in nutritional value. However, the production of these bioactive constituents under standard laboratory conditions is inefficiency due to the silent expression of their relevant genes. Coculture, as an important activation strategy that simulates the natural living conditions of macrofungi, can activate silent genes or clusters through interspecific interactions. Coculturing not only can trigger the biosynthesis of diverse secondary metabolites and enzymes of macrofungi, but is also useful for uncovering the mechanisms of fungal interspecific interactions and novel gene functions. In this paper, coculturing among macrofungi or between macrofungi and other microorganisms, the triggering and upregulation of secondary metabolites and enzymes, the potential medicinal applications, and the fungal–fungal interaction mechanisms are reviewed. Finally, future challenges and perspectives in further advancing coculture systems are discussed.
Guihong Yu; Yuman Sun; Heyang Han; Xiu Yan; Yu Wang; Xiaoxuan Ge; Bin Qiao; Lingling Tan. Coculture, An Efficient Biotechnology for Mining the Biosynthesis Potential of Macrofungi via Interspecies Interactions. Frontiers in Microbiology 2021, 12, 1 .
AMA StyleGuihong Yu, Yuman Sun, Heyang Han, Xiu Yan, Yu Wang, Xiaoxuan Ge, Bin Qiao, Lingling Tan. Coculture, An Efficient Biotechnology for Mining the Biosynthesis Potential of Macrofungi via Interspecies Interactions. Frontiers in Microbiology. 2021; 12 ():1.
Chicago/Turabian StyleGuihong Yu; Yuman Sun; Heyang Han; Xiu Yan; Yu Wang; Xiaoxuan Ge; Bin Qiao; Lingling Tan. 2021. "Coculture, An Efficient Biotechnology for Mining the Biosynthesis Potential of Macrofungi via Interspecies Interactions." Frontiers in Microbiology 12, no. : 1.
Butadiene is a platform chemical used as an industrial feedstock for the manufacture of automobile tires, synthetic resins, latex and engineering plastics. Currently, butadiene is predominantly synthesized as a byproduct of ethylene production from non-renewable petroleum resources. Although the idea of biological synthesis of butadiene from sugars has been discussed in the literature, success for that goal has so far not been reported. As a model system for methanol assimilation, Methylobacterium extorquens AM1 can produce several unique metabolic intermediates for the production of value-added chemicals, including crotonyl-CoA as a potential precursor for butadiene synthesis. In this work, we focused on constructing a metabolic pathway to convert crotonyl-CoA into crotyl diphosphate, a direct precursor of butadiene. The engineered pathway consists of three identified enzymes, a hydroxyethylthiazole kinase (THK) from Escherichia coli, an isopentenyl phosphate kinase (IPK) from Methanothermobacter thermautotrophicus and an aldehyde/alcohol dehydrogenase (ADHE2) from Clostridium acetobutylicum. The Km and kcat of THK, IPK and ADHE2 were determined as 8.35 mM and 1.24 s−1, 1.28 mM and 153.14 s−1, and 2.34 mM and 1.15 s−1 towards crotonol, crotyl monophosphate and crotonyl-CoA, respectively. Then, the activity of one of rate-limiting enzymes, THK, was optimized by random mutagenesis coupled with a developed high-throughput screening colorimetric assay. The resulting variant (THKM82V) isolated from over 3000 colonies showed 8.6-fold higher activity than wild-type, which helped increase the titer of crotyl diphosphate to 0.76 mM, corresponding to a 7.6% conversion from crotonol in the one-pot in vitro reaction. Overexpression of native ADHE2, IPK with THKM82V under a strong promoter mxaF in M. extorquens AM1 did not produce crotyl diphosphate from crotonyl-CoA, but the engineered strain did generate 0.60 μg/mL of intracellular crotyl diphosphate from exogenously supplied crotonol at mid-exponential phase. These results represent the first step in producing a butadiene precursor in recombinant M. extorquens AM1. It not only demonstrates the feasibility of converting crotonol to key intermediates for butadiene biosynthesis, it also suggests future directions for improving catalytic efficiency of aldehyde/alcohol dehydrogenase to produce butadiene precursor from methanol.
Jing Yang; Chang-Tai Zhang; Xiao-Jie Yuan; Min Zhang; Xu-Hua Mo; Ling-Ling Tan; Li-Ping Zhu; Wen-Jing Chen; Ming-Dong Yao; Bo Hu; Song Yang. Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor. Microbial Cell Factories 2018, 17, 194 .
AMA StyleJing Yang, Chang-Tai Zhang, Xiao-Jie Yuan, Min Zhang, Xu-Hua Mo, Ling-Ling Tan, Li-Ping Zhu, Wen-Jing Chen, Ming-Dong Yao, Bo Hu, Song Yang. Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor. Microbial Cell Factories. 2018; 17 (1):194.
Chicago/Turabian StyleJing Yang; Chang-Tai Zhang; Xiao-Jie Yuan; Min Zhang; Xu-Hua Mo; Ling-Ling Tan; Li-Ping Zhu; Wen-Jing Chen; Ming-Dong Yao; Bo Hu; Song Yang. 2018. "Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor." Microbial Cell Factories 17, no. 1: 194.