Nanoparticles are widely used for biomedical applications such as vaccine, drug delivery, diagnostics, and therapeutics. This study aims to reveal the influence of nanoparticle surface functionalization on protein corona formation from blood serum and plasma and the subsequent effects on the innate immune cellular responses. To achieve this goal, the surface chemistry of silica nanoparticles of 20 nm diameter was tailored via plasma polymerization with amine, carboxylic acid, oxazolines, and alkane functionalities. The results of this study show significant surface chemistry-induced differences in protein corona composition, which reflect in the subsequent inflammatory consequences. Nanoparticles rich with carboxylic acid surface functionalities increased the production of pro-inflammatory cytokines in response to higher level of complement proteins and decreased the number of lipoproteins found in their protein coronas. On another hand, amine rich coatings led to increased expressions of anti-inflammatory markers such as arginase. The findings demonstrate the potential to direct physiological responses to nanomaterials via tailoring their surface chemical composition.

Here we report the development of slef-sterilizing dissolving microneedles, a promising vehicle for vaccine and drug delivery.

Polyoxazolines arise as a promising new class of polymers for biomedical applications, but creating oxzoline-based coatings via conventional methods is challenging. Herein, nanoscale polyoxazoline coatings were generated via a single step plasma deposition process. The effects of plasma deposition conditions on the film stability, structure and chemical group density were investigated. Detailed examination of the physical and chemical properties of plasma deposited polyoxazoline via XPS, FTIR, contact angle and ellipsometry unravels the complex functionality of the films. Partial retention of the oxazoline ring facilitates a covalent reaction with the carboxylic acid groups present on nanoparticles and biomolecules. Surface bound proteins effectively retain their bioactivity, therefore a vast range of potential applications unlocks for plasma deposited polyoxazoline coatings in the field of biosensing, medical arrays and diagnosis.