There are challenges associated with design and development of affinity biosensors due to the complicated multiphysics nature of the system. Understanding the binding interaction between target molecules and immobilized receptors and its kinetics is a crucial step to develop robust and reliable biosensor technologies. Evaluation of binding kinetics in biosensors becomes more important and challenging for clinical samples with a complex matrix. Despite drastic advancements in biosensor technologies, having a practical perception of the binding kinetics has remained a critical bottleneck due to limited fundamental understanding. This Review aims to provide a comprehensive discussion on concepts and advances developed so far for the perception of binding kinetics in affinity biosensors. Here, modeling approaches and measurement techniques are presented to characterize the binding interactions in biosensor technologies, while the effect of fouling and secondary factors in the binding interactions will be discussed in the concept of kinetics. This Review will investigate the existing research gaps and potential opportunities in the perception of binding kinetics and challenges to develop robust and reliable biosensors.

The use of silicon nanowire resonators in nanoelectromechanical systems for new-generation sensing and communication devices faces integration challenges with higher-order structures. Monolithic and deterministic integration of such nanowires with the surrounding microscale architecture within the same thick crystal is a critical aspect for the improvement of throughput, reliability and device functionality. A monolithic and IC-compatible technology based on a tuned combination of etching and protection processes was recently introduced yielding silicon nanowires within a 10 μ m-thick device layer. Motivated by its success, the implications of the technology regarding the electromechanical resonance are studied within a particular setting, where the resonator is co-fabricated with all terminals and tuning electrodes. Frequency response is measured via piezoresistive readout with frequency down-mixing. Measurements indicate mechanical resonance with frequencies as high as 100 MHz exhibiting a Lorentzian behavior with proper transition to nonlinearity, while Allan deviation on the order of 3–8 ppm is achieved. Enabling the fabrication of silicon nanowires in thick silicon crystals using conventional semiconductor manufacturing, the present study thus demonstrates an alternative pathway to bottom-up and thin silicon-on-insulator approaches for silicon nanowire resonators.

Material extrusion additive manufacturing (ME-AM) techniques have been recently introduced for core–shell polymer manufacturing. Using ME-AM for core–shell manufacturing offers improved mechanical properties and dimensional accuracy over conventional 3D-printed polymer. Operating parameters play an important role in forming the overall quality of the 3D-printed manufactured products. Here we use numerical simulations within the framework of computation fluid dynamics (CFD) to identify the best combination of operating parameters for the 3D printing of a core–shell polymer strand. The objectives of these CFD simulations are to find strands with an ultimate volume fraction of core polymer. At the same time, complete encapsulations are obtained for the core polymer inside the shell one. In this model, the deposition flow is controlled by three dimensionless parameters: (i) the diameter ratio of core material to the nozzle, $d/D$; (ii) the normalised gap between the extruder and the build plate, $t/D$; (iii) the velocity ratio of the moving build plate to the average velocity inside the nozzle, $V/U$. Numerical results of the deposited strands’ cross-sections demonstrate the effects of controlling parameters on the encapsulation of the core material inside the shell and the shape and size of the strand. Overall we find that the best operating parameters are a diameter ratio of $d/D=0.7$, a normalised gap of $t/D=1$, and a velocity ratio of $V/U=1$.

The wellhead flow control valve bodies which are the focal point of this failure case study were installed in some of the upstream facilities of Khangiran's sour gas wells. These valve bodies have been operating satisfactorily for 3 years in wet H2S environment before some pits and cracks were detected in all of them during the periodical technical inspections. One failed valve body was investigated by chemical and microstructural analytical techniques to find out the failure cause and provide preventive measures. The valve body alloy was A216-WCC cast carbon steel. During investigation many cracks were observed on the inner surface of the valve body grown from the surface pits. The results indicate that flow control valve body failed due to combination of hydrogen induced corrosion cracking (HICC) and sulfide stress corrosion cracking (SSCC). According to HIC and SSC laboratory tests and also with regard to cost of engineering materials, it was evident that the best alternative for the valve body alloy is A217-WC9 cast Cr–Mo steel