Leaf nutrient content and its stoichiometric relationships (N/P ratio) are essential for photosynthesis and plant growth and development. Previous studies on leaf nutrient-related functional traits have mainly focused on the species level and regional scale, but fewer studies have investigated the distribution patterns of the leaf N and P contents (LN, LP) and N/P ratios (N/P) in communities and their controlling factors at a large scale; therefore, we used LN, LP, and N/P data at 69 sites from 818 forests in China. The results showed significant differences (p < 0.05) in the LN, LP, and N/P at different life forms (tree, shrub, and herb). Neither LN, LP, nor N/P ratios showed significant patterns of latitudinal variation. With the increase in temperature and rainfall, the LN, LP, and leaf nutrient contents increased significantly (p < 0.001). Across life forms, LN at different life forms varied significantly and was positively correlated with soil P content (p < 0.001). The explanatory degree of climatic factors in shaping the spatial variation patterns of LN and N/P was higher than that of the soil nutrient factors, and the spatial variation patterns of the leaf nutrient traits of different life forms were shaped by the synergistic effects of climatic factors and soil nutrient factors.

The allocation of biomass reflects a plant’s resource utilization strategy and is significantly influenced by climatic factors. However, it remains unclear how climate factors affect the aboveground and belowground biomass allocation patterns on macro scales. To address this, a study was conducted using aboveground and belowground biomass data for 486 species across 294 sites in China, investigating the effects of climate change on biomass allocation patterns. The results show that the proportion of belowground biomass in the total biomass (BGBP) or root-to-shoot ratio (R/S) in the northwest region of China is significantly higher than that in the southeast region. Significant differences (p < 0.05) were found in BGBP or R/S among different types of plants (trees, shrubs, and herbs plants), with values for herb plants being significantly higher than shrubs and tree species. On macro scales, precipitation and soil nutrient factors (i.e., soil nitrogen and phosphorus content) are positively correlated with BGBP or R/S, while temperature and functional traits are negatively correlated. Climate factors contribute more to driving plant biomass allocation strategies than soil and functional trait factors. Climate factors determine BGBP by changing other functional traits of plants. However, climate factors influence R/S mainly by affecting the availability of soil nutrients. The results quantify the productivity and carbon sequestration capacity of terrestrial ecosystems and provide important theoretical guidance for the management of forests, shrubs, and herbaceous plants.

Bacillus subtilis J-15 is a plant growth-promoting rhizobacteria isolated from the soil rhizosphere of cotton and is resistant to cotton verticillium wilt. This study evaluated the effects of metabolites of J-15 (J-15-Ms), including mycosubtilin, on plant growth using Arabidopsis and cotton plants. The results showed that J-15-Ms promoted Arabidopsis seeding growth at lower concentrations of 0.2 μg/mL but inhibited the growth at higher concentrations, such as 20 μg/mL. Similar results were obtained in cotton. Thus, J-15-Ms-treated plants showed low-concentration-induced growth promotion and high-concentration-induced growth inhibition. The J-15-Ms components were analyzed by liquid chromatography–mass spectrometry. Correlation analysis using the J-15 genomic databases suggested that J-15 may synthesize indoleacetic acid via the indole-3-pymvate pathway and indole-3-acetamide pathway. Treatment with mycosubtilin, a purified peptide from J-15-Ms, showed that the peptide promoted Arabidopsis growth at a low concentration (0.1 μg/mL) and inhibited plant growth at high concentrations (higher than 1 μg/mL), which also significantly increased plant lateral root number. Transcriptomic analysis showed that mycosubtilin might promote lateral root development and inhibit plant primary root growth by regulating the expression of the plant hormone signaling pathway. This study reveals the mechanism of Bacillus subtilis J-15 in affecting plant growth.