The development of biosensors based on DNA hybridization requires a more precise knowledge of the thermodynamics of the hybridization at a solid interface. In particular, the selectivity of hybridization can be affected by a lot of parameters such as the single‐strand (ss)DNA density, the pH, the ionic strength or the temperature. The melting temperature, T _m, is in part a function of the ionic strength and of the temperature and therefore provides a useful variable in the control of the selectivity and sensitivity of a DNA chip. The electrochemical technique has been used to determine the T _m values when the probe is tethered by a DNA self‐assembled monolayer (SAM). We have built a special thin layer cell, which allows the recording of the cyclic voltammogram under controlled temperature conditions. T _m has been determined by recording the thermogram (current versus temperature) of a redox indicator on a double‐stranded hybrid (dsDNA) modified electrode and comparison with the corresponding ssDNA response. T _m of supported DNA varies linearly with the ionic strength. The stability of the SAMs has been considered and comparison between T _m in solution and on a solid support has been discussed.