Vegetation carbon sequestration (CS) is critical for mitigating climate change in urban agglomerations, yet its driving mechanisms remain poorly understood in rapidly urbanizing regions. This study introduces an integrated attribution and influence analysis framework, GWR-RF-SEM, to quantitatively assess the driving forces, mechanisms, and pathways of CS using multi-source remote sensing data at the county scale within the Yangtze River Delta Urban Agglomeration (YRDUA), China, from 2001 to 2020. Our results reveal an overall increase in CS across 70.14% districts in the YRDUA, with municipal districts exhibiting significantly lower CS compared to the outside districts. Photosynthesis and human activities emerged as the dominant drivers, collectively accounting for 73.1% of CS variation, significantly surpassing the influence of climate factors. Although most factors influenced urban vegetation CS either directly or indirectly, photosynthesis, afforestation, and urban green space structure were identified as the primary direct drivers of CS enhancement in both districts. Notably, we found significant spatial heterogeneity in CS drivers between municipal districts and the outside districts, highlighting the need for targeted strategies to enhance CS efficiency. These findings advance our understanding of urban vegetation CS mechanisms, providing essential support for the enhancement of nature-based solutions depending on ecosystem services under urbanization and climate change.

Brownification denotes increasing water color, partly caused by increasing dissolved organic matter of terrestrial origin in freshwater. Brownification has become a wide-spread environmental problem because water color alters the physicochemical environment and biological communities in aquatic ecosystems. However, our understanding of its ecological effects on aquatic macrophytes is limited. Here, an indoor mesocosm experiment with a common submerged macrophyte, Potamogeton crispus, along an increasing gradient of brownification was conducted over a period of 42 days. Results showed that P. crispus was able to overcome low degrees of brownification owing to the plasticity in morphological and physiological traits and P. crispus growth even benefitted from the concomitant nutrients along with brownification. However, the macrophyte growth was negatively affected by a 10-fold increase in water color beyond its current level. Additionally, collapse in antioxidant systems and potent photosynthesis inhibition implied that P. crispus could not adapt to the low-light stress generated under the high degree of brownification. Epiphytic bacteria are more sensitive to brownification than their hosts. Any degree of brownification initially caused a decrease in microbial diversity of epiphytic biofilm, whereafter the concomitant nutrients under brownification favored the growth of epiphytic microorganisms. The shading effect of a large number of epiphytic biofilms under brownification may further aggravate the low-light stress on macrophytes. Overall, the study provides new insights into the comprehensive effects and underlying mechanisms of brownification on macrophytes.