Soil microorganisms promote the recovery of contaminated soil by influencing the cyclic transformation of various substances. In this study, we investigated the impact of mercury pollution on the structure, composition, and main populations of soil microbial communities using a high-throughput sequencing method and observed that mercury pollution significantly influenced the diversity, structure, and distribution pattern of microbial communities. Furthermore, during mercury pollution, the Shannon and Chao indices decreased for the bacterial communities and increased for the fungal communities. Mercury pollution mainly reduced the relative abundances of Proteobacteria (16.2–30.6%), Actinomycetes (24.7–40.8%), and other dominant bacterial phyla. The relative abundance of Ascomycota decreased by 17.4% and 16.7% in alkaline and neutral soils, respectively, whereas the relative abundance of unclassified_k_Fungi increased by 26.1% and 28.6%, respectively. In acidic soil, Ascomycota increased by 106.3% and unclassified_k_Fungi decreased by 71.2%. The results of redundancy and correlation analyses suggested that soil microbial diversity was significantly correlated with soil properties such as pH, cation exchange capacity, soil organic carbon, and total nitrogen (p < 0.05) under different treatments. Our findings highlight the impact of Hg pollution on soil microbial communities, thereby providing a theoretical foundation for the bioremediation of soil Hg pollution.

Land-use systems (LUSs) and soil types (STs) are strongly related to factors that influence soil degradation and carbon (C) loss. However, the way in which land use and soil type affects the soil organic C (SOC) pools, and soil aggregation in the Sanjiang Plain, has not been thoroughly investigated. Therefore, this study aimed to investigate soil physic–ochemical properties, soil aggregates, and C management index (CMI) in three different LUSs (grassland, dryland, and paddy field) under two STs (meadow soil and albic soil) of the Sanjiang Plain in northeast China. A total of 60 composite soil samples were collected for laboratory analyses. The results were as follows: soil properties were affected by LUS and ST, especially soil chemical properties; ST had no significant effect on soil aggregates but significantly affected its SOC content, while LUS had a significant effect on soil aggregates (p < 0.01), except for small macro-aggregates (2–0.25 mm); the mean weight diameter (MWD) and SOC in meadow soil was significantly different under different land uses, with grassland being the highest and dryland the lowest. However, there was no significant difference in albic soil. The heterogeneity of grassland, dryland, and paddy field showed that different LUSs had particular effects on SOC and its active components because LUS had significant effects on C pool index (CPI) and CMI, but ST and its interaction had no significant effects on CPI and CMI. Overall, the results showed that LUS was an important factor affecting CMI in the Sanjiang Plain, rather than ST, and the paddy field CMI was optimal in the Sanjiang Plain.

Microplastic pollution in agricultural soil threatens soil quality and human health which has attracted extensive attention worldwide. However, there is no uniform standard for microplastic extraction methods and the identification of microplastic component in different typical agricultural soils. In this study, an artificial simulation adding experiment was used in eight typical agricultural soil samples in China. The aim of the study was to use different methods for extraction, comparing the extraction rates of four microplastics and their influence on polymer identification using ATR- FTIR. The two separate methods were oil extraction (water + oil and saturated NaCl solution + oil), and density method (saturated NaCl solution). The four types of microplastics include polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), and polyethylene (PE). We found that the oil extraction method effectively extracted four types of microplastics in agricultural soils, which varied from 83.33% to 100.00%. However, the extraction rate of PET under the oil extraction method and PP under the density method from Southern laterite area was lower than other soils. The presence of iron and aluminum ions influenced the extraction rates of microplastics in the Southwest laterite area. With the increase in microplastic density, the extraction rates of the density method decrease. The oil extraction methods with the cleaning of residual oil were recommended for the higher density microplastics. The density method was recommended for the lighter microplastics in agricultural soils. However, these two extraction methods were not ideal to extract the microplastics from the Southern laterite area and the appropriate extraction methods for laterite need to be further studied in the future. Our results can provide technical support for the extraction treatment and scientific microplastic pollution control of typical agricultural soils with different erosion areas.