The application of flowering plants is the basis of urban forest construction. A newly-found flowering hyperaccumulator is crucial for remediating urban contaminated soil sustainably by cadmium (Cd). This study evaluated growth responses, Cd uptake and bioaccumulation characteristics of seven urban flowering plants. Based on growth responses of these plants, Calendula officinalis L. showed high tolerance to at least 100 mg kg⁻¹ Cd, in terms of significant increase in biomass and with no obvious changes in height. After 60 d exposure to 100 mg kg⁻¹ Cd, the accumulated Cd in shoots of the plant reached 279.51 ± 13.67 μg g⁻¹ DW, which is above the critical value defined for a hyperaccumulator (100 μg g⁻¹ DW for Cd). Meanwhile, the plant could accumulate Cd to as much as 926.68 ± 29.11 μg g⁻¹ DW in root and 1206.19 ± 23.06 μg g⁻¹ DW in plant, and had higher Cd uptake and bioaccumulation values. According to these traits, it is shown that Calendula officinalis L. can become a potential Cd-hyperaccumulator for phytoremediation. By contrast, Dianthus caryophyllus L. is very sensitive to Cd stress in terms of significantly decreased biomass, height and Cd uptake, indicating the plant is considered as a Cd-bioindicator.

Graphene oxide (GO), as a novel carbon-based nanomaterial (CBN), has been widely applied to every respect of social life due to its unique composite properties. The widespread use of GO inevitably promotes its interaction with heavy metal cadmium (Cd), and influences its functional behavior. However, little information is available on the effects of GO on greening hyperaccumulators under co-occurring Cd. In this study, we chose a typical greening hyperaccumulator (Lonicera japonica Thunb.) to show the effect of GO on Cd accumulation, growth, net photosynthesis rate (P_n), carbon sequestration and oxygen release functions of the plant under Cd stress. The different GO-Cd treatments were set up by (0, 10, 50 and 100 mg L⁻¹) GO and (0, 5 and 25 mg L⁻¹) Cd in solution culture. The maximum rate of Cd accumulation in the roots and shoots of the plant were increased by 10 mg L⁻¹ GO (exposed to 5 mg L⁻¹ Cd), indicating that low-concentration GO (10 mg L⁻¹) combined with low-concentration Cd (5 mg L⁻¹) might stimulate the absorption of Cd by L. japonica. Under GO treatments without Cd, the dry weight of root and shoot biomass, P_n value, carbon sequestration per unit leaf area and oxygen release per unit leaf area all increased in various degrees, especially under 10 mg L⁻¹ GO, were 20.67%, 12.04%, 35% and 28.73% higher than the control. Under GO-Cd treatments, it is observed that the cooperation of low-concentration GO (10 mg L⁻¹) and low-concentration Cd (5 mg L⁻¹) could significantly stimulate Cd accumulation, growth, photosynthesis, carbon sequestration and oxygen release functions of the plant. These results indicated that suitable concentrations of GO could significantly alleviate the effects of Cd on L. japonica, which is helpful for expanding the phytoremediation application of greening hyperaccumulators faced with coexistence with environment of nanomaterials and heavy metals.

“Hormesis” is considered a dose–response phenomenon mainly observed at hyperaccumulator plants under heavy metals stress. In this study, the effects of electric fields on hormesis responses in Lonicera japonica Thunb. under cadmium (Cd) treatments were investigated by assessing the plant growth and photosynthetic characteristics. Under Cd treatments without electric fields, the parameters of plant growth and photosynthetic characteristics increased significantly when exposed to 5 mg L⁻¹ Cd, and decreased slightly when exposed to 25 mg L⁻¹ Cd, showing an inverted U-shaped trend, which confirmed that low concentration Cd has a hormesis effect on L. japonica. Under electric fields, different voltages significantly promoted the inverted U-shaped trend of the hormesis effect on the plant, especially by 2 V cm⁻¹ voltage. Under 2 V cm⁻¹ voltage, the dry weight of the root and leaf biomass exposed to 5 mg L⁻¹ Cd increased significantly by 38.38% and 42.14%, and the photosynthetic pigment contents and photosynthetic parameters were also increased significantly relative to the control, indicating that a suitable electric field provides better improvements for the hormesis responses of the plant under Cd treatments. The synergistic benefits of the 5 mg L⁻¹ Cd and 2 V cm⁻¹ electric field in terms of the enhanced hormesis responses of growth and photosynthetic characteristics could contribute to the promoted application of electro-phytotechnology.

The multi-system of electro-phytotechnology using a woody ornamental cadmium (Cd) hyperaccumulator (Lonicera japonica Thunb.) is a new departure for environmental remediation. The effects of four electric field conditions on Cd accumulation, growth, and photosynthesis of L. japonica under four Cd treatments were investigated. Under 25 and 50 mg L⁻¹ Cd treatments, Cd accumulation in L. japonica was enhanced significantly compared to the control and reached 1110.79 mg kg⁻¹ in root and 428.67 mg kg⁻¹ in shoots influenced by the electric field, especially at 2 V cm⁻¹, and with higher bioaccumulation coefficient (BC), translocation factor (TF), removal efficiency (RE), and the maximum Cd uptake, indicating that 2 V cm⁻¹ voltage may be the most suitable electric field for consolidating Cd-hyperaccumulator ability. It is accompanied by increased root and shoots biomass and photosynthetic parameters through the electric field effect. These results show that a suitable electric field may improve the growth, hyperaccumulation, and photosynthetic ability of L.japonica. Meanwhile, low Cd supply (5 mg L⁻¹) and medium voltage (2 V cm⁻¹) improved plant growth and photosynthetic capacity, conducive to the practical application to a plant facing low concentration Cd contamination in the real environment.

The carbon sequestration and oxygen release of landscape plants are dominant ecological service functions, which can play an important role in reducing greenhouse gases, improving the urban heat island effect and achieving carbon peaking and carbon neutrality. In the present study, we are choosing Lonicera japonica Thunb. as a model plant to show the effects of Cd stress on growth, photosynthesis, carbon sequestration and oxygen release characteristics. Under 5 mg kg⁻¹ of Cd treatment, the dry weight of roots and shoots biomass and the net photosynthetic rate (P_N) in L. japonica had a significant increase, and with the increase in Cd treatment concentration, the dry weight of roots and shoots biomass and P_N in the plant began to decrease. When the Cd treatment concentration was up to 125 mg kg⁻¹, the dry weight of root and shoots biomass and P_N in the plant decreased by 5.29%, 1.94% and 2.06%, and they had no significant decrease compared with the control, indicating that the plant still had a good ability for growth and photoenergy utilization even under high concentrations of Cd stress. The carbon sequestration and oxygen release functions in terms of diurnal assimilation amounts (P), carbon sequestration per unit leaf area (WCO₂), oxygen release per unit leaf area (WO₂), carbon sequestration per unit land area (PCO₂) and oxygen release per unit land area (PO₂) in L. japonica had a similar change trend with the photosynthesis responses under different concentrations of Cd treatments, which indicated that L. japonica as a landscaping Cd-hyperaccumulator, has a good ability for carbon sequestration and oxygen release even under high concentrations of Cd stress. The present study will provide a useful guideline for effectively developing the ecological service functions of landscaping hyperaccumulators under urban Cd-contaminated environment.