Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP.

Many oil and gas fields are nearing production cessation and will require decommissioning, with the preferred method being complete infrastructure removal in most jurisdictions. However, decommissioning in situ, leaving some disused components in place, is an option that may be agreed to by the regulators and reservoir titleholders in some circumstances. To understand this option's viability, the environmental impacts and risks of any residual contaminants assessed. Mercury, a contaminant of concern, is naturally present in hydrocarbon reservoirs, may contaminate offshore processing and transmission infrastructure, and can biomagnify in marine ecosystems. Mercury's impact is dependent on its speciation, concentration, and the exposure duration. However, research characterising and quantifying the amount of mercury in offshore infrastructure and the efficacy of decontamination is limited. This review describes the formation of mercury-contaminated products within oil and gas infrastructure, expected exposure pathways after environmental release, possible impacts, and key research gaps regarding the ecological risk of in situ decommissioned contaminated infrastructure. Suggestions are made to overcome these gaps, improving the in situ mercury quantification in infrastructure, understanding environmental controls on, and forecasting of, mercury methylation and bioaccumulation, and the cumulative impacts of multiple stressors within decommissioned infrastructures.

The fish embryo acute toxicity (FET) test with the zebrafish (Danio rerio) embryo according to OECD TG 236 was originally developed as an alternative test method for acute fish toxicity testing according to, e.g., OECD TG 203. Given the versatility of the protocol, however, the FET test has found application beyond acute toxicity testing as a common tool in environmental hazard and risk assessment. Whereas the standard OECD guideline is restricted to four core endpoints (coagulation as well as lack of somite formation, heartbeat, and tail detachment) for simple, rapid assessment of acute toxicity, further endpoints can easily be integrated into the FET test protocol. This has led to the hypothesis that an extended FET test might allow for the identification of different classes of toxicants via a “fingerprint” of morphological observations. To test this hypothesis, the present study investigated a set of 18 compounds with highly diverse modes of action with respect to acute and sublethal endpoints. Especially at higher concentrations, most observations proved toxicant-unspecific. With decreasing concentrations, however, observations declined in number, but gained in specificity. Specific observations may at best be made at test concentrations ≤ EC₁₀. The existence of a “fingerprint” based on morphological observations in the FET is, therefore, highly unlikely in the range of acute toxicity, but cannot be excluded for experiments at sublethal concentrations.

In the aim to determine neurotoxicity, new methods are being validated, including tests and test batteries comprising in vitro and in vivo approaches. Alternative test models such as the zebrafish (Danio rerio) embryo have received increasing attention, with minor modifications of the fish embryo toxicity test (FET; OECD TG 236) as a tool to assess behavioral endpoints related to neurotoxicity during early developmental stages. The spontaneous tail movement assay, also known as coiling assay, assesses the development of random movement into complex behavioral patterns and has proven sensitive to acetylcholine esterase inhibitors at sublethal concentrations. The present study explored the sensitivity of the assay to neurotoxicants with other modes of action (MoAs). Here, five compounds with diverse MoAs were tested at sublethal concentrations: acrylamide, carbaryl, hexachlorophene, ibuprofen, and rotenone. While carbaryl, hexachlorophene, and rotenone consistently induced severe behavioral alterations by ~ 30 h post fertilization (hpf), acrylamide and ibuprofen expressed time- and/or concentration-dependent effects. At 37–38 hpf, additional observations revealed behavioral changes during dark phases with a strict concentration-dependency. The study documented the applicability of the coiling assay to MoA-dependent behavioral alterations at sublethal concentrations, underlining its potential as a component of a neurotoxicity test battery.

Subsea pipelines carrying well fluids from hydrocarbon fields accumulate mercury. If the pipelines (after cleaning and flushing) are abandoned in situ, their degradation may release residual mercury into the environment. To justify pipeline abandonment, decommissioning plans include environmental risk assessments to determine the potential risk of environmental mercury. These risks are informed by environmental quality guideline values (EQGVs) governing concentrations in sediment or water above which mercury toxicity may occur. However, these guidelines may not consider e.g., the bioaccumulation potential of methylated mercury. Therefore, EQGVs may not protect humans from exposure if applied as the sole basis for risk assessments. This paper outlines a process to assess the EQGVs' protectiveness from mercury bioaccumulation, providing preliminary insights to questions including how to (1) determine pipeline threshold concentrations, (2) model marine mercury bioaccumulation, and (3) determine exceedance of the methylmercury tolerable weekly intake (TWI) for humans. The approach is demonstrated with a generic example using simplifications to describe mercury behaviour and a model food web. In this example, release scenarios equivalent to the EQGVs resulted in increased marine organism mercury tissue concentrations by 0-33 %, with human dietary methylmercury intake increasing 0-21 %. This suggests that existing guidelines may not be protective of biomagnification in all circumstances. The outlined approach could inform environmental risk assessments for asset-specific release scenarios but must be parameterised to reflect local environmental conditions when tailored to local factors.

Environmental context: The oil and gas industry has a significant liability in decommissioning offshore infrastructure. Following decommissioning, subsea pipelines could be left on the seabed to provide artificial reefs. Mercury is a contaminant of concern which could remain within pipelines. There are gaps in our knowledge on how mercury moves through the marine environment. We review the current science and identify future research needs to understand potential impacts from mercury in subsea pipelines which will better inform decommissioning activities globally. In the coming years, the oil and gas industry will have a significant liability in decommissioning offshore infrastructure such as subsea pipelines. The policies around decommissioning vary depending on regional policies and laws. In Australia, the ‘base case’ for decommissioning is removal of all property and the plugging and abandonment of wells in line with the Offshore Petroleum and Greenhouse Gas Storage (OPGGS) Act 2006. Options other than complete removal may be considered where the titleholder can demonstrate that the alternative decommissioning activity delivers equal or better environmental outcomes compared to complete removal and meets all requirements under the OPGGS Act and regulations. Recent research has demonstrated that decommissioning in situ can have significant environmental benefits by forming artificial reefs, increasing marine biodiversity, and providing a potential fishery location. An issue, which has been given less attention, is around contaminants remaining within decommissioned infrastructure and their potential risks to the marine environment. Mercury is a contaminant of concern known to be present in some oil and gas pipelines, but the potential long-term impacts on marine ecosystems are poorly understood. We present a synthesis of information on mercury cycling in the marine environment including key drivers of methylation in sediments and ocean waters, existing models to predict methylmercury concentrations in sediments, and toxicological effects to marine biota. We discuss the applicability of existing water and sediment quality guidelines, and the associated risk assessment frameworks to decommissioning offshore infrastructure contaminated with mercury. Globally, research is needed to provide a comprehensive risk assessment framework for offshore infrastructure decommissioning. We recommend future areas of research to improve our understanding of the potential risks associated with mercury in subsea oil and gas pipelines.