Recent advancements in stem cell research forge them into one of the most promising sources for cell therapy applications. Quality monitoring in stem cell culture is essential for ensuring consistency, viability, and therapeutic efficacy. Traditional methods involve periodic sampling for conducting endpoint assays such as cell viability, proliferation, and differentiation using microscopy and flow cytometry, which are labor‐intensive and often lack the real‐time monitoring of the processes for scale‐up applications. This paper explores artificial intelligence (AI)‐driven approaches for real‐time quality control, integrating machine vision, predictive modeling, and sensor‐based monitoring. AI models analyze high‐resolution imaging and multi‐sensor data to dynamically track critical quality attributes (CQAs), including cell morphology, proliferation rate, differentiation potential, environmental stability (pH, oxygen, and nutrient levels), genetic integrity, and contamination risks. These models enable automated anomaly detection, differentiation tracking, and adaptive culture optimization. By leveraging real‐time feedback systems and multi‐omics integration, AI‐driven techniques enhance scalability, reproducibility, and process automation in stem cell biomanufacturing. This review outlines current advancements, challenges, and future directions in AI‐assisted quality monitoring and highlights its potential to improve fully automated, scalable production of stem cell lines for clinical translation and regulatory compliance in regenerative medicine.

Industrial facilities collect large volumes of data, store them according to prescribed protocols, and then interpret them for process decision‐making. Several sources and types of error contaminate these data for various reasons, but especially because they come from unreliable or unpredictable instruments. Data (or signal) processing corrects measurement errors to improve fidelity. Here, we highlight decision‐making applications and signal processing methods. To fully appreciate the state‐of‐the‐art, we interviewed plant data experts and software developers in the pulp and paper industry to examine how they apply signal processing methods in the context of decision‐making, including the value of process data, how these data are used, and the major barriers that prevent plants from using data. Process experts clean data thoroughly with basic approaches compared to the advanced techniques available in the recent literature. The interviews demonstrate that decisions in industry are primarily based on steady‐state process operating data. Challenges and barriers that prevent the use of process data to their full potential relate to resource limitations (people, time, and money), an entrenched culture, and access to recent technology. In practice, experts consider, implicitly or explicitly, data that represent the process operating under steady‐state conditions. A plant model that represents steady‐state operations is easier to interpret, is presented in a form that is usable by plant operators, and in this way, better enables decision‐making.