Umami and sweet sensations provide animals with important dietary information for detecting and consuming nutrients, whereas bitter sensation helps animals avoid potentially toxic or harmful substances. Enormous progress has been made toward animal sweet/umami taste receptor (Tas1r) and bitter taste receptor (Tas2r). However, information about amphibians is mainly scarce. This study attempted to delineate the repertoire of Tas1r/Tas2r genes by searching for currently available genome sequences in 14 amphibian species. This study identified 16 Tas1r1, 9 Tas1r2, and 9 Tas1r3 genes to be intact and another 17 Tas1r genes to be pseudogenes or absent in the 14 amphibians. According to the functional prediction of Tas1r genes, two species have lost sweet sensation and seven species have lost both umami and sweet sensations. Anurans possessed a large number of intact Tas2rs, ranging from 39 to 178. In contrast, caecilians possessed a contractive bitter taste repertoire, ranging from 4 to 19. Phylogenetic and reconciling analysis revealed that the repertoire of amphibian Tas1rs and Tas2rs was shaped by massive gene duplications and losses. No correlation was found between feeding preferences and the evolution of Tas1rs in amphibians. However, the expansion of Tas2rs may help amphibians adapt to both aquatic and terrestrial habitats. Bitter detection may have played an important role in the evolutionary adaptation of vertebrates in the transition from water to land.

The gut microbiota is integral to an animal’s physiology, influencing nutritional metabolism, immune function, and environmental adaptation. Despite the significance of gut microbiota in wild rodents, the Korean field mouse (Apodemus peninsulae) and the gray red-backed vole (Myodes rufocanus) remain understudied. To address this, a metagenomic sequencing analysis of the gut microbiome of these sympatric rodents in northeast China’s temperate forests was conducted. Intestinal contents were collected from A. peninsulae and M. rufocanus within the Mudanfeng National Nature Reserve. High-throughput sequencing elucidated the gut microbiome’s composition, diversity, and functional pathways. Firmicutes, Bacteroidetes, and Proteobacteria were identified as the dominant phyla, with M. rufocanus showing greater microbiome diversity. Key findings indicated distinct gut bacterial communities between the species, with M. rufocanus having a higher abundance of Proteobacteria. The gut microbiota of A. peninsulae and M. rufocanus differed marginally in functional profiles, specifically in the breakdown of complex carbohydrates, which might reflect their distinct food preferences albeit both being herbivores with a substantial dietary overlap. The investigation further elucidated gut microbiota’s contributions to energy metabolism and environmental adaptation mechanisms. This study aligns with information on rodent gut microbiota in literature and highlights the two understudied rodent species, providing comparative data for future studies investigating the role of gut microbiota in wildlife health and ecosystem functioning.

The habitat landscape pattern of Mrs Hume’s pheasant in Jinzhongshan, northwestern Guangxi, was studied using field survey data and the LANDSAT satellite images by the ArcGIS 10.8 and Fragstats 3.3 software. The results showed that the Jinzhongshan region covers 38,716.6 hm², including 1708 patches and 11 landscape types. Although the area of farmland and village only occupies 10%, their number and density have led Jinzhongshan habitats to fragment. The degree of connection of suitable habitat was found to be relatively low, and seven landscape indices were below 0.5, which implied that the extent of habitat fragmentation in Jinzhongshan for Mrs Hume’s Pheasant is very high. The fragmentation index of Jinzhongshan Nature Reserve is 0.9887, landscape connectivity is 1.861, and AWS index is 425.3024. The broad-leaved forest, considered a matrix in the Jinzhongshan area, was the dominant landscape type controlling structure, function, and dynamic changes. The total suitable habitat of Mrs Hume’s pheasant (Syrmaticus humiae) was determined to be 29,552.3 hm², accounting for 76.3% of the total study area; the suitable habitat of Mrs Hume’s pheasant in Jinzhongshan Nature Reserve was determined to be 16,990.1 hm², accounting for 81.14% of the protected area. It is absolutely necessary and urgent to strengthen the management and protection of Mrs Hume’s pheasant’s habitat to control its fragmentation. Therefore, we have provided some useful advice, such as habitat restoration and corridor reconstruction, which are beneficial to the conservation of Mrs Hume’s pheasant in this sensitive region.

Microsatellites or simple sequence repeats (SSRs) are prevalent across various organisms' genomes. However, their distribution patterns and evolutionary dynamics in reptile genomes are rarely studied systematically. We herein conducted a comprehensive analysis of SSRs in the genomes of 36 reptile species. Our findings revealed that the total number of SSRs ranged from 1,840,965 to 7,664,452, accounting for 2.16%–8.19% of the genomes analyzed. The relative density ranged from 21,567.82 to 81,889.41 bp per megabase (Mbp). The abundance of different SSR categories followed the pattern of imperfect SSR (I‐SSR) > perfect SSR (P‐SSR) > compound SSR (C‐SSR). A significant positive correlation was observed between the number of SSRs and genome size ( p = 0.0034), whereas SSR frequency ( p = 0.013) or density ( p = 0.0099) showed a negative correlation with genome size. Furthermore, no correlation was found between SSR length and genome size. Mononucleotide repeats were the most common P‐SSRs in crocodilians and turtles, whereas mononucleotides, trinucleotides, or tetranucleotides were the most common P‐SSRs in snakes, lizards, and tuatara. P‐SSRs of varying motif sizes showed nonrandom distribution across different genic regions, with AT‐rich repeats being predominant. The genomic SSR content of the squamate lineage ranked the highest in abundance and variability, whereas crocodilians and turtles showed a slowly evolving and reduced microsatellite landscape. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that genes harboring P‐SSRs in the coding DNA sequence regions were primarily involved in the regulation of transcription and translation processes. The SSR dataset generated in this study provides potential candidates for functional analysis and calls for broader‐scale analyses across the evolutionary spectrum.

In the present study, summer habitat preference of Mrs Hume’s Pheasant (Syrmaticus humiae) was studied in Jinzhong Mountain Nature Reserve, Guangxi Province. Eight birds were equipped with radio transmitters and habitat characteristics of experiment and control quadrat sites, respectively, were measured. Our results indicated that: 200 m to 400 m distance to habitat edge, less than 200 m distance to water, over 400 m distance to human habitation, over 601 m distance to the road, over 60% tree cover, less than 40% shrub cover, less than 20% herb cover, over 60% leaf litter cover, height of tree over 10.1 m, tree density over 20 individuals per quadrat, shrub height over 2.1 m, shrub density less than 10 individuals per quadrat, height of herb species over 0.5 m, herb density less than 10 Individuals per quadrat, diameter of tree over 30 cm, and abundant food were the habitat characteristics. The elevation, herb density, and height of tree and shrub exhibited no significant differences (), but other factors showed significant differences between quadrat sites and control quadrat plot (). Results of a principal components analysis (PCA) demonstrated that the first five components accounted for 64.282% of the total variance. Food availability in different habitat patches is the vital factor affecting habitat selection by Mrs Hume’s Pheasant; the other factors showed no significant effects on habitat selection by Mrs Hume’s Pheasant in the subtropical forest. This suggests that habitat selection behavior of Mrs Hume’s Pheasant is in fact a trade-off between obtaining food and prey escape, which is an adaptive behavior and a life strategy of this near-threatened bird species.

A habitats comprises a range of environmental features that provide adequate resources for wildlife to survive. Consequently, the criteria of habitat selection by animals, combines a wider spectrum of both environmental and other factors, with major prerequisites being food resources, availability of shelter and suitable conditions for reproduction. The den habitat selection of the Chinese bamboo rat (Rhizomys sinensis) was studied in Yuanbaoshan Nature Reserve, Guangxi China. The Vanderloeg and Scavia selectivity index (Ei) indicates that the Chinese bamboo rat shows a preference for all habitat features except for slope, slope position and distance to water (P>0.05). Chi-square goodness of fit test showed that canopy density, shelter from wind, distance between shrubs, shrub density, elevation, southern aspect, human disturbance and food abundance, were the preferred features (P1000 m away from human habitation. An eastern and southern sloping direction, a 20°^~40° slope and an elevation range of >1800m were more frequently used. The Chinese bamboo rat prefers habitats lying above 1000m in elevation with high shrub density (with shrub separation under 0.1m). Principle component analysis indicates that concealment factors, geographical factors, interference factors, food factors, and water factors affect the den habitat character of the Chinese bamboo rat. Our results support the notion that the habitat preferences of the Chinese bamboo rat are a tradeoff between suitable habitat features and avoidance of potential human interactions.