Journal of Medical Internet Research - International Scientific Journal for Medical Research, Information and Communication on the Internet #Preprint #PeerReviewMe: Warning: This is a unreviewed preprint. Readers are warned that the document has not been peer-reviewed by expert/patient reviewers or an academic editor, may contain misleading claims, and is likely to undergo changes before final publication, if accepted, or may have been rejected/withdrawn. Readers with interest and expertise are encouraged to sign up as peer-reviewer, if the paper is within an open peer-review period. Please cite this preprint only for review purposes or for grant applications and CVs (if you are the author). Background: Social media has numerous applications for health promotion and disease prevention efforts, and the benefits of using social media for public health communication have been documented. Generating participant engagement is vital for effective health promotion, and increases the likelihood of effecting sustainable behavior change. However, there is limited evidence on effective strategies for engaging Latino immigrant youth using social media. The authors, as part of the Avance Center for the Advancement of Immigrant/Refugee Health, implemented Adelante, a branded primary prevention program (2012-2017) to address risk factors for co-occurring substance use, sexual risk and interpersonal violence among Latino immigrant adolescents, ages 12-19 in a Washington, DC suburb. Objective: We established the Adelante Facebook fan page in October of 2013, and the Adelante social marketing campaign utilized this platform for campaign activities from September 2015 to September 2016. This study had the following aims: 1) to characterize Adelante participant Facebook reach and engagement; 2) to identify post content and features that resulted in greater user engagement; and 3) to examine the content and features of the most engaging posts to identify patterns and compare to all posts. Methods: We used Facebook Insights metrics to examine reach and post engagement of Adelante Facebook page fans (n=743). Data consisted of Facebook fan page posts between October 1, 2013 and September 30, 2016 (n=871). We developed a two-phased mixed-methods analytical plan and coding scheme, and explored the association between post content/features and Post Engagement by conducting separate one-way ANOVA tests. For variables with samples sizes below 50 for an IV, we used the non-parametric Wilcoxon-Mann Whitney test. We used a p-value of p<.05 to determine statistical significance. Results: The Adelante Facebook page reached a total of 739 fans with 871 posts. There were a total of 34,318 post clicks, 473 post comments, 9,080 post likes/reactions, and 617 post shares. Post content categories that were statistically significantly associated with user engagement included: Adelante program updates (F=24.654, P<.001); youth achievement showcases (P=.001); news links (F=12.501, P<.001); and prevention topics, including substance abuse (P<.001), safe sex (P=.01), STDs (P<.001), and violence/fighting (P=.04). Post features that were associated with user engagement included: Spanish language (F=3.546, P=.02); portrayal of mixed gender youth (F=10.122, P=.002) in groups (F=16.613, P<.001); boosting (F=44.821, P<.001); and synchronicity with the social marketing campaign (F=8.125, P<.001). Conclusions: Youth were particularly interested in social media posts insofar as they were an extension of the in-person activities. We recommend that programs include online efforts that intersect with in-person programming as a strategy to augment youth engagement. Additionally, social media prevention messages and posts should be created in collaboration with the intended audience, thus including content and features that are most likely to result in youth engagement.