Benzotriazoles (BTRs) are a class of benzoheterocyclic chemicals that are frequently used as metal-corrosive inhibitors, both in industry and daily use. However, the exposure effect information on BTRs remains relatively limited. In this study, an integrated metabolomic and transcriptomic approach was utilized to evaluate the effect of three BTRs, benzotriazole, 6-chloro-1-hydroxi-benzotriazole, and 1-hydroxy-benzotriazole, in the mouse liver with results showing disrupted basal metabolic processes and vitamin and cofactor metabolism after 28 days. The expression of several genes that are related to the inflammatory response and aryl hydrocarbon receptor pathways, such as Gstt2 and Arntl, was altered by the exposure to BTRs. Exposure to BTRs also affected metabolites and genes that are involved in the immune system and xenobiotic responses. The altered expression of several cytochrome P450 family genes reveal a potential detoxification mechanism in the mouse liver. Taken together, our findings provide new insights into the multilayer response of the mouse liver to BTRs exposure as well as a resource for further exploration of the molecular mechanisms by which the response occurs.

Plants face various biotic and abiotic stress factors during their growth and development, among which, drought is a serious adverse factor that affects yield and quality in agriculture and forestry. Several transcription factors are involved in regulating plant responses to drought stress. In this study, the B-box (BBX) transcription factor CoBBX24 was cloned from Camellia oleifera. This gene encodes a 241-amino-acid polypeptide containing two B-box domains at the N-terminus. A phylogenetic analysis revealed that CoBBX24 and CsBBX24 from Camellia sinensis are in the same branch, with their amino acid sequences being identical by 96.96%. CoBBX24 was localized to the nucleus and acted as a transcriptional activator. The overexpression of CoBBX24 in Arabidopsis heightened its drought tolerance along with a relatively high survival rate, and the rate of water loss in the OX-CoBBX24 lines was observably lower than that of the wild-type. Compared to the wild-type, the root lengths of the OX-CoBBX24 lines were significantly inhibited with abscisic acid. Leaf senescence was delayed in the OX-CoBBX24 lines treated with abscisic acid. The expression of genes related to leaf senescence and chlorophyll breakdown (e.g., SAG12, SAG29, NYC1, NYE1, and NYE2) was downregulated in the OX-CoBBX24 lines. This study indicated that CoBBX24 positively regulates the drought tolerance in Arabidopsis through delayed leaf senescence.