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Manal M. Alkhulaifi
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

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Journal article
Published: 23 May 2021 in Sustainability
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The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.

ACS Style

Rajan Walhe; Sham Diwanay; Milind Patole; R. Sayyed; Hind Al-Shwaiman; Manal Alkhulaifi; Abdallah Elgorban; Subhan Danish; Rahul Datta. Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt. Sustainability 2021, 13, 5853 .

AMA Style

Rajan Walhe, Sham Diwanay, Milind Patole, R. Sayyed, Hind Al-Shwaiman, Manal Alkhulaifi, Abdallah Elgorban, Subhan Danish, Rahul Datta. Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt. Sustainability. 2021; 13 (11):5853.

Chicago/Turabian Style

Rajan Walhe; Sham Diwanay; Milind Patole; R. Sayyed; Hind Al-Shwaiman; Manal Alkhulaifi; Abdallah Elgorban; Subhan Danish; Rahul Datta. 2021. "Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt." Sustainability 13, no. 11: 5853.

Journal article
Published: 02 April 2021 in Medicina
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Background and Objectives: The multidrug resistant (MDR) bacterial pathogenic infection is one of the chief worldwide public health threat to humanity. The development of novel antibiotics against MDR Gram negative bacteria has reduced over the last half century. Research is in progress regarding the treatment strategies that could be engaged in combination with antibiotics to extend the duration of these life-saving antibacterial agents. The current study was therefore planned to assess the synergistic effects of bovine lactoferrin (bLF) in combination with different antibiotics that are conventionally used. This synergism would provide a newer therapeutic choice against MDR pathogens. LF is present in mucosal secretions, vastly in milk. LF is considered an important constituent in host defense. In previous reports, LF has been co-administered as a combination antibiotic therapy. Materials and Methods: This study included synergistic (LF + appropriate antibiotic) exposure against 147 locally encountered bacterial pathogens, which were completely characterized strains. The anti-biofilm effects and the outcome of bLF on minimum inhibitory concentrations (MICs) of antibacterials on clinical MDR bacterial pathogens were determined by standard techniques. Results: In our study, synergism of bLF with antibacterial agents were reproducible and found to be significant. LF on its own had an important effect of inhibiting the biofilm production of some significant bacterial pathogens. Conclusion: The results of this study provides useful data on the antibacterial potential of the combination of LF with antibiotics against drug resistant pathogens.

ACS Style

Mohammed Al-Mogbel; Godfred Menezes; Mohamed Elabbasy; Manal Alkhulaifi; Ashfaque Hossain; Mushtaq Khan. Effect of Synergistic Action of Bovine Lactoferrin with Antibiotics on Drug Resistant Bacterial Pathogens. Medicina 2021, 57, 343 .

AMA Style

Mohammed Al-Mogbel, Godfred Menezes, Mohamed Elabbasy, Manal Alkhulaifi, Ashfaque Hossain, Mushtaq Khan. Effect of Synergistic Action of Bovine Lactoferrin with Antibiotics on Drug Resistant Bacterial Pathogens. Medicina. 2021; 57 (4):343.

Chicago/Turabian Style

Mohammed Al-Mogbel; Godfred Menezes; Mohamed Elabbasy; Manal Alkhulaifi; Ashfaque Hossain; Mushtaq Khan. 2021. "Effect of Synergistic Action of Bovine Lactoferrin with Antibiotics on Drug Resistant Bacterial Pathogens." Medicina 57, no. 4: 343.

Journal article
Published: 29 March 2021 in Molecules
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Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

ACS Style

Elsayim Rasha; Alothman Monerah; Alkhulaifi Manal; Ali Rehab; Doud Mohammed; Elnagar Doaa. Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae. Molecules 2021, 26, 1919 .

AMA Style

Elsayim Rasha, Alothman Monerah, Alkhulaifi Manal, Ali Rehab, Doud Mohammed, Elnagar Doaa. Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae. Molecules. 2021; 26 (7):1919.

Chicago/Turabian Style

Elsayim Rasha; Alothman Monerah; Alkhulaifi Manal; Ali Rehab; Doud Mohammed; Elnagar Doaa. 2021. "Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae." Molecules 26, no. 7: 1919.

Journal article
Published: 23 September 2020 in Saudi Journal of Biological Sciences
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The present work aimed to synthesis silver nanoparticles (AgNPs) using biological waste products Citrus limon peels, its characterization, antimicrobial activities and the cytotoxic effect of the synthesized green AgNPs. Characterization of the prepared AgNPs showed the formation of spherical, and few agglomerated AgNPs forms as measured by UV–visible spectrophotometer. The average size of the prepared AgNPs was 59.74 nm as measured by DLS technique. The spectrum of the synthesized AgNPs was observed at 3 KeV using the EDX. On the other hand, FTIR analysis of the green synthesized AgNPs showed the presence of alcohols, phenolics, mono-substituted alkynes, aliphatic primary amines, sodium salt, amino acid, or SiOH alcohol groups. The antimicrobial studies of the formed AgNPs showed positive activity against most of the studied human pathogenic bacteria with varying degrees. Finally, the evaluation of the cytotoxic effect of the green synthesized AgNPs were done using two types of cell lines, human breast cancer cell line (MCF-7) and human colon carcinoma cell line (HCT-116). The results revealed the concentration has a direct correlation with cell viability. The 50% inhibitory concentration (IC50) of MCF-7 cell line was in of 23.5 ± 0.97 µL/100 µL, whereas the HCT-116 cell line was in 37.48 ± 5.93 µL/100 µL.

ACS Style

Manal M. Alkhulaifi; Jamilah H. Alshehri; Moudi A. Alwehaibi; Manal A. Awad; Nouf M. Al-Enazi; Noura S. Aldosari; Ashraf A. Hatamleh; Neveen Abdel- Raouf. Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties. Saudi Journal of Biological Sciences 2020, 27, 3434 -3441.

AMA Style

Manal M. Alkhulaifi, Jamilah H. Alshehri, Moudi A. Alwehaibi, Manal A. Awad, Nouf M. Al-Enazi, Noura S. Aldosari, Ashraf A. Hatamleh, Neveen Abdel- Raouf. Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties. Saudi Journal of Biological Sciences. 2020; 27 (12):3434-3441.

Chicago/Turabian Style

Manal M. Alkhulaifi; Jamilah H. Alshehri; Moudi A. Alwehaibi; Manal A. Awad; Nouf M. Al-Enazi; Noura S. Aldosari; Ashraf A. Hatamleh; Neveen Abdel- Raouf. 2020. "Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties." Saudi Journal of Biological Sciences 27, no. 12: 3434-3441.

Journal article
Published: 07 August 2020 in Green Processing and Synthesis
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The adaptive nature of algae results in producing unique chemical components that are gaining attention due to their efficiency in many fields and abundance. In this study, we screened the phytochemicals from the brown alga Hydroclathrus clathratus and tested its ability to produce silver nanoparticles (AgNPs) extracellularly for the first time. Lastly, we investigated its biological activity against a variety of bacteria. The biosynthesized nanoparticles were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and energy-dispersive spectroscopy. The biological efficacy of AgNPs was tested against eighteen different bacteria, including seven multidrug-resistant bacteria. Phytochemical screening of the alga revealed the presence of saturated and unsaturated fatty acids, sugars, carboxylic acid derivatives, triterpenoids, steroids, and other components. Formed AgNPs were stable and ranged in size between 7 and 83 nm and presented a variety of shapes. Acinetobacter baumannii, Staphylococcus aureus, Methicillin-resistant S. aureus (MRSA), and MDR A. baumannii were the most affected among the bacteria. The biofilm formation and development assay presented a noteworthy activity against MRSA, with an inhibition percentage of 99%. Acknowledging the future of nano-antibiotics encourages scientists to explore and enhance their potency, notably if they were obtained using green, rapid, and efficient methods.

ACS Style

Raghad R. Alzahrani; Manal M. Alkhulaifi; Nouf M. Al-Enazi. In vitro biological activity of Hydroclathrus clathratus and its use as an extracellular bioreductant for silver nanoparticle formation. Green Processing and Synthesis 2020, 9, 416 -428.

AMA Style

Raghad R. Alzahrani, Manal M. Alkhulaifi, Nouf M. Al-Enazi. In vitro biological activity of Hydroclathrus clathratus and its use as an extracellular bioreductant for silver nanoparticle formation. Green Processing and Synthesis. 2020; 9 (1):416-428.

Chicago/Turabian Style

Raghad R. Alzahrani; Manal M. Alkhulaifi; Nouf M. Al-Enazi. 2020. "In vitro biological activity of Hydroclathrus clathratus and its use as an extracellular bioreductant for silver nanoparticle formation." Green Processing and Synthesis 9, no. 1: 416-428.

Erratum
Published: 24 July 2020 in Materials Letters
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ACS Style

Manal A. Awad; Manal M. Alkhulaifi; Moudi Alwehaibi; Jamilah Alshehri; Khalid Ortashi; Rabia Qindeel; Noura Aldosari; Awatif Hendi; Hajar Aldakheel. Corrigendum to “Dielectric properties of red sand silver nanoparticles” [Mater. Lett. 268 (2020) 127626–127629]. Materials Letters 2020, 276, 128322 .

AMA Style

Manal A. Awad, Manal M. Alkhulaifi, Moudi Alwehaibi, Jamilah Alshehri, Khalid Ortashi, Rabia Qindeel, Noura Aldosari, Awatif Hendi, Hajar Aldakheel. Corrigendum to “Dielectric properties of red sand silver nanoparticles” [Mater. Lett. 268 (2020) 127626–127629]. Materials Letters. 2020; 276 ():128322.

Chicago/Turabian Style

Manal A. Awad; Manal M. Alkhulaifi; Moudi Alwehaibi; Jamilah Alshehri; Khalid Ortashi; Rabia Qindeel; Noura Aldosari; Awatif Hendi; Hajar Aldakheel. 2020. "Corrigendum to “Dielectric properties of red sand silver nanoparticles” [Mater. Lett. 268 (2020) 127626–127629]." Materials Letters 276, no. : 128322.

Journal article
Published: 18 March 2020 in Animals
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This study evaluated the effects of feed additives on the growth, blood biochemistry and intestinal health of broiler chicks. A total of 378 of broiler chicks (Ross 308) were randomly allotted to seven groups. Chicks were fed a basal diet with 0.0 (control negative), 0.0 (control positive), 0.1, 0.5, 0.12, 0.5 + 0.12 and 0.2 g Kg−1 of Maxus, CloStat, Sangrovit Extra, CloStat + Sangrovit Extra and Gallipro Tech, respectively for 35 days. After 15 days, the chicks were inoculated with Clostridium perfringens. All feed additives were found to enhance growth performance and feed efficiency. The best feed conversion ratio was found in the Negative Control, CloStat + Sangrovit Extra and Gallipro Tect groups, respectively. A notable increase in villus length, total villus area, small intestine weight, ilium weight and total lesion score was found in chicks supplemented with Bacillus subtilis. Besides, the dietary inclusion of phytobiotic compounds showed potential in reducing the serum Alanine aminotransferase (ALT) concentration and increasing the glucose levels. All intestine and liver histopathological signs were reduced in chicks fed a probiotic-supplemented diet. Our findings indicate that supplementation with probiotics and phytobiotics alone or in combined form can be used to enhance performance, intestine health and blood constituents against C. perfringens infection in broiler chicks.

ACS Style

Elsayed O. S. Hussein; Shamseldein H. Ahmed; Alaeldein M. Abudabos; Mashael R. Aljumaah; Manal M. Alkhlulaifi; Mohamed A. Nassan; Gamaleldin M. Suliman; Mohammed A. E. Naiel; Ayman A. Swelum. Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens. Animals 2020, 10, 507 .

AMA Style

Elsayed O. S. Hussein, Shamseldein H. Ahmed, Alaeldein M. Abudabos, Mashael R. Aljumaah, Manal M. Alkhlulaifi, Mohamed A. Nassan, Gamaleldin M. Suliman, Mohammed A. E. Naiel, Ayman A. Swelum. Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens. Animals. 2020; 10 (3):507.

Chicago/Turabian Style

Elsayed O. S. Hussein; Shamseldein H. Ahmed; Alaeldein M. Abudabos; Mashael R. Aljumaah; Manal M. Alkhlulaifi; Mohamed A. Nassan; Gamaleldin M. Suliman; Mohammed A. E. Naiel; Ayman A. Swelum. 2020. "Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens." Animals 10, no. 3: 507.

Short communication
Published: 09 March 2020 in Materials Letters
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Nanoparticles exhibit novel physical and chemical attributes suitable for the field of electronics, optics, and electromagnetics. This study investigates the dielectric properties of silver nanoparticles (AgNPs) which are synthesized using red sand suspension. AgNPs are expected to play an important role in the diverse field of applications and they are widely used as paste conductors. In this investigation, AgNP’s dielectric parameters are determined between 20 Hz and 3 MHz in the recurrence run. The AgNPs show solid dielectric scattering, relating to the locale of alpha unwinding inside the expressed recurrence go, which is portrayed as bizarre scattering of frequencies. The conductivity expanded quickly at high frequencies, and the dielectric consistent is quickly diminishing because of the inclination of the dipoles in the AgNPs to arrange themselves in the low recurrence go toward the applied field. The estimation of the dielectric steady remained practically consistent in the high-recurrence run.

ACS Style

Manal A. Awad; Manal Alkulaifi; Moudi Alwehaibi; Jamilah AlShehri; Khalid Ortashi; Rabia Qindeel; Noura Aldosari; Awatif Hendi; Hajar Aldakheel. Dielectric properties of red sand silver nanoparticles. Materials Letters 2020, 268, 127626 .

AMA Style

Manal A. Awad, Manal Alkulaifi, Moudi Alwehaibi, Jamilah AlShehri, Khalid Ortashi, Rabia Qindeel, Noura Aldosari, Awatif Hendi, Hajar Aldakheel. Dielectric properties of red sand silver nanoparticles. Materials Letters. 2020; 268 ():127626.

Chicago/Turabian Style

Manal A. Awad; Manal Alkulaifi; Moudi Alwehaibi; Jamilah AlShehri; Khalid Ortashi; Rabia Qindeel; Noura Aldosari; Awatif Hendi; Hajar Aldakheel. 2020. "Dielectric properties of red sand silver nanoparticles." Materials Letters 268, no. : 127626.

Journal article
Published: 01 February 2020 in Anaerobe
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This study was designed to determine the incidence of Clostridium perfringens and their toxin genes in children with autism spectrum disorder (ASD), and determine the antimicrobial susceptibility of C. perfringens isolates. A hundred and fourteen fecal samples were obtained from children aged 3-12 years old (57 samples from ASD children and 57 from healthy controls). Children were divided into four groups based on their gastrointestinal (GI) symptoms as follows: ASD group with and without GI symptoms, and control group with and without GI symptoms. Selective anaerobic media and VITEK®2 ANC ID card were used for isolation and identification of C. perfringens from fecal samples. Antimicrobial susceptibility of C. perfringnes isolates were performed using (E-Test) strips against clindamycin, penicillin and metronidazole antibiotics. Conventional PCR was used to detect the alpha toxin gene (Cpa) and the beta-2 toxin gene (Cpb2). Genetic Analyzer 3130Xi was used to confirm the sequencing of Cpb2 gene. Our findings indicated that 38.6% of ASD group samples had a significantly (p = 0.003) higher incidence of C. perfringens than that of the control group (14%). The highest incidence of C. perfringens was found in the ASD group with GI symptoms (53.8%; p = 0.001). C. perfringens isolated from the ASD group (54.5%) were significantly (p = 0.047) more resistant to clindamycin than those isolated from the control group (12.5%). The C. perfringens isolates from the ASD and the control group showed 95.5% and 100% susceptibility to penicillin, respectively. All C. perfringens isolates of ASD and control group were susceptible to metronidazole. The Cpa toxin gene was also detected in all the C. perfringens isolates of ASD and control group, both with and without GI symptoms. Cpb2 toxin gene showed 100% incidence in ASD samples with GI symptoms and in the control groups both with or without GI symptoms, while it was present at significantly lower levels (25%) in the ASD samples without GI symptoms (p = 0.001). Our findings suggests that a high incidence of C. perfringens and its toxin gene (Cpb2) are associated with the GI complications in ASD which may affect the severity of the disease.

ACS Style

Maryam K. Alshammari; Manal M. AlKhulaifi; Dunia A. Al Farraj; Ali Somily; Ahmed M. Albarrag. Incidence of Clostridium perfringens and its toxin genes in the gut of children with autism spectrum disorder. Anaerobe 2020, 61, 102114 .

AMA Style

Maryam K. Alshammari, Manal M. AlKhulaifi, Dunia A. Al Farraj, Ali Somily, Ahmed M. Albarrag. Incidence of Clostridium perfringens and its toxin genes in the gut of children with autism spectrum disorder. Anaerobe. 2020; 61 ():102114.

Chicago/Turabian Style

Maryam K. Alshammari; Manal M. AlKhulaifi; Dunia A. Al Farraj; Ali Somily; Ahmed M. Albarrag. 2020. "Incidence of Clostridium perfringens and its toxin genes in the gut of children with autism spectrum disorder." Anaerobe 61, no. : 102114.

Journal article
Published: 01 January 2020 in The Journal of Poultry Science
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Antibiotic growth promoters (AGPs) have been used for many years as supplements in various livestock diets, including those for poultry. However, the use of AGPs in feed was also associated with an increasing number of antibiotic-resistant bacteria in livestock. In this study, the in vitro antibacterial efficacies of eight commercially available non-AGPs suitable for use in poultry were investigated. Assessments included a combination of antibacterial activity assays and estimations of the minimal inhibitory and bactericidal concentrations along with scanning electron microscopy analysis. The results showed that the probiotic, CloStat® exerted a bacteriostatic effect against all tested bacteria, namely Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus, and Clostridium perfringens, whereas Gallipro Tect® and Bacillus Blend® demonstrated bacteriostatic activity towards most of the pathogens tested. Other commercial non-AGPs, Sangrovit®, Fysal®, and Mix oil blend® showed a stronger or equal antibacterial activity compared to the positive control (AGP Maxus® G100) againsts all bacteria tested, except C. perfringens. Nor-Spice AB® and Varium™ did not show any significant effect against the tested bacteria. Several of the tested AGP substitutes exhibited good antibacterial efficiency against pathogenic bacteria and thus may be good candidates for second-stage in vivo investigations into reducing pathogen colonization in broilers.

ACS Style

Mashael R. Aljumaah; Manal M. Alkhulaifi; Alaeldein M. Abudabos. In vitro Antibacterial Efficacy of Non-Antibiotic Growth Promoters in Poultry Industry. The Journal of Poultry Science 2020, 57, 45 -54.

AMA Style

Mashael R. Aljumaah, Manal M. Alkhulaifi, Alaeldein M. Abudabos. In vitro Antibacterial Efficacy of Non-Antibiotic Growth Promoters in Poultry Industry. The Journal of Poultry Science. 2020; 57 (1):45-54.

Chicago/Turabian Style

Mashael R. Aljumaah; Manal M. Alkhulaifi; Alaeldein M. Abudabos. 2020. "In vitro Antibacterial Efficacy of Non-Antibiotic Growth Promoters in Poultry Industry." The Journal of Poultry Science 57, no. 1: 45-54.

Journal article
Published: 25 November 2019 in Materials
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Nanomedicine is growing due to the development of new medical diagnostic tools and new nanostructure-based therapies that exert direct biological action or function as pharmacological carriers. Nanoparticles (NPs) synthesis provides an eco-friendly approach for different applications. Among NPs, silver NPs (AgNPs) are gaining considerable research interest due to their broad range of activity and their usability in the medical and biotechnology fields. In this study, a new AgNP synthesis method was developed using an aqueous pigeon dropping (PD) extract in silver nitrate (AgNO3). The rapid of AgNPs yield was detected visually. Analysis of UV-vis spectroscopy, energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS) and electron microscopy (TEM) transmission showed a spherical or near spherical shape of AgNPs with mean size of 135 nm. AgNPs antimicrobial activities (anti-bacterial and anti-fungal) were determined using agar well diffusion method. These NPs further screened for anticancer activity in vitro using A-549 and MCF-7 cell lines. The results showed that the inhibition zone for the obtained PD AgNPs versus Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus were 26, 18, 17 and 15 mm, respectively. PD AgNPs showed the highest antifungal effect against Aspergillus flavus and the lowest effect against Penicillium griseofulvum. In vitro anti-cancer activities showed that the inhibitory concentration of 50% (IC50) of AgNPs was 10.3 ± 1.15 and 12.19 ± 0.75 µg mL−1 against A-549 and MCF-7 cancer cell lines, respectively.

ACS Style

Manal A. Awad; Manal M. Alkhulaifi; Noura S. Aldosari; Shaykha Alzahly; Ali Aldalbahi. Novel Eco-Synthesis of PD Silver Nanoparticles: Characterization, Assessment of Its Antimicrobial and Cytotoxicity Properties. Materials 2019, 12, 3890 .

AMA Style

Manal A. Awad, Manal M. Alkhulaifi, Noura S. Aldosari, Shaykha Alzahly, Ali Aldalbahi. Novel Eco-Synthesis of PD Silver Nanoparticles: Characterization, Assessment of Its Antimicrobial and Cytotoxicity Properties. Materials. 2019; 12 (23):3890.

Chicago/Turabian Style

Manal A. Awad; Manal M. Alkhulaifi; Noura S. Aldosari; Shaykha Alzahly; Ali Aldalbahi. 2019. "Novel Eco-Synthesis of PD Silver Nanoparticles: Characterization, Assessment of Its Antimicrobial and Cytotoxicity Properties." Materials 12, no. 23: 3890.

Journal article
Published: 01 February 2018 in Saudi Journal of Biological Sciences
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Silver nanoparticles (AgNPs) are gaining considerable importance due to their attractive physicochemical properties for many applications. In the present study, (Ag NPs) were synthesized by the reduction of aqueous solutions of silver nitrate (AgNO3) with powder and solvent extracts of Padina pavonia (brown algae). The obtained nanoparticles exhibited high stability, rapid formation of the biogenic process (2 min -3 h), small size (49.58–86.37 nm) (the diameter of formed nanoparticles was measured by TEM and DLS) and variable shapes (spherical, triangular, rectangle, polyhedral and hexagonal). Preliminary characterization of nanoparticles was monitored by using UV–visible spectroscopy (UV–vis), Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS) and finally by Fourier Transform Infrared spectroscopy (FTIR). The ratios of converted Ag NPs were recorded as 88.5; 86.2 and 90.5% in case of P. pavonia powder. extract and chloroform extract, respectively.

ACS Style

Neveen Abdel-Raouf; Nouf Al-Enazi; Ibraheem Borie Mohammad Ibraheem; Reem Mohammed Alharbi; Manal Mohammed Alkhulaifi. Biosynthesis of silver nanoparticles by using of the marine brown alga Padina pavonia and their characterization. Saudi Journal of Biological Sciences 2018, 26, 1207 -1215.

AMA Style

Neveen Abdel-Raouf, Nouf Al-Enazi, Ibraheem Borie Mohammad Ibraheem, Reem Mohammed Alharbi, Manal Mohammed Alkhulaifi. Biosynthesis of silver nanoparticles by using of the marine brown alga Padina pavonia and their characterization. Saudi Journal of Biological Sciences. 2018; 26 (6):1207-1215.

Chicago/Turabian Style

Neveen Abdel-Raouf; Nouf Al-Enazi; Ibraheem Borie Mohammad Ibraheem; Reem Mohammed Alharbi; Manal Mohammed Alkhulaifi. 2018. "Biosynthesis of silver nanoparticles by using of the marine brown alga Padina pavonia and their characterization." Saudi Journal of Biological Sciences 26, no. 6: 1207-1215.

Journal article
Published: 06 August 2016 in Saudi Journal of Biological Sciences
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Dissemination of carbapenem resistance via Enterobacteriaceae, particularly among Klebsiella pneumoniae and Escherichia coli, is a major public health concern. Rapid methods for determining antimicrobial susceptibility are important to ensure adequate and appropriate use of antimicrobial agents and to limit the spread of these bacteria. In the current study, we compared the rapidity, sensitivity and specificity of traditional methods and molecular-based Xpert Carba-R PCR assay to identify sixty isolates, (26 E. coli and 34 K. pneumoniae). The specificity of MicroScan was 100% while sensitivity to ertapenem (ERT), imipenem (IMI), and meropenem (MER) was 93%, 68.9%, and 55.17%, respectively. For the modified Hodge test, the specificity was 96.77% and sensitivity was 89.65%. Although some results of phenotypic assays matched with the definite PCR identification, some results were misleading. Out of the 29 positive PCR samples, three samples of K. pneumoniae were negative for the MHT and one E. coli sample was MHT positive but negative for the PCR. Nine samples were positive for the PCR but were determined as carbapenem sensitive by MicroScan. While MicroScan and MHT requires several hours and multi-steps to obtain results, Xpert Carba-R PCR assay takes less than an hour. Therefore, we recommend using Gene xpert Carba-R assay for the optimal carbapenemnase detection with reducing material, manpower and cost. Also it is important to know the type of carbapenemase is present.

ACS Style

Maryam AlTamimi; Ali AlSalamah; Manal AlKhulaifi; Hisham AlAjlan. Comparison of phenotypic and PCR methods for detection of carbapenemases production by Enterobacteriaceae. Saudi Journal of Biological Sciences 2016, 24, 155 -161.

AMA Style

Maryam AlTamimi, Ali AlSalamah, Manal AlKhulaifi, Hisham AlAjlan. Comparison of phenotypic and PCR methods for detection of carbapenemases production by Enterobacteriaceae. Saudi Journal of Biological Sciences. 2016; 24 (1):155-161.

Chicago/Turabian Style

Maryam AlTamimi; Ali AlSalamah; Manal AlKhulaifi; Hisham AlAjlan. 2016. "Comparison of phenotypic and PCR methods for detection of carbapenemases production by Enterobacteriaceae." Saudi Journal of Biological Sciences 24, no. 1: 155-161.

Journal article
Published: 31 July 2009 in Saudi Journal of Biological Sciences
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Staphylococcus aureus is one of the major causes of community and hospital-acquired infections. Bacteriophage considered as a major risk factor acquires S. aureus new virulence genetic elements. A total number of 119 S. aureus isolated from different specimens obtained from (RKH) were distinguished by susceptibility to 19 antimicrobial agents, phage typing, and PCR amplification for mecA gene. All of MRSA isolates harbored mecA gene, except three unique isolates. The predominant phage group is belonging to the (mixed group). Phage group (II) considered as an epidemiological marker correlated to β-lactamase hyper producer isolates. MRSA isolates indicated high prevalence of phage group (II) with highly increase for phage types (Ø3A), which were correlated to the skin. Phage types (Ø80/Ø81) played an important roll in Community Acquired Methicillin Resistant S. aureus (CAMRSA). Three outpatients MRSA isolates had low multiresistance against Bacitracin (Ba) and Fusidic acid (FD), considered as CAMRSA isolates. It was detected that group I typed all FD-resistant MSSA isolates. Phage groups (M) and (II) were found almost to be integrated for Gentamycin (GN) resistance especially phage type (Ø95) which relatively increased up to 20% in MRSA. Tetracycline (TE) resistant isolates typed by groups (II) and (III) in MSSA. Only one isolate resistant to Sulphamethoxazole/Trimethoprim (SXT) was typed by (III/V) alone in MSSA. MRSA isolates resistant to Chloramphenicol (C) and Ba were typed by all groups except (V). It could be concluded that (PERSA) S. aureus isolates from the wound that originated and colonized, and started to build up multi-resistance against the topical treatment antibiotics. In this study, some unique sporadic isolates for both MRSA and MSSA could be used as biological, molecular and epidemiological markers such as prospective tools.

ACS Style

M. Al-Khulaifi Manal; M. Amin Aref Nagwa; Ali A. Al Salamah. Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus. Saudi Journal of Biological Sciences 2009, 16, 37 -49.

AMA Style

M. Al-Khulaifi Manal, M. Amin Aref Nagwa, Ali A. Al Salamah. Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus. Saudi Journal of Biological Sciences. 2009; 16 (1):37-49.

Chicago/Turabian Style

M. Al-Khulaifi Manal; M. Amin Aref Nagwa; Ali A. Al Salamah. 2009. "Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus." Saudi Journal of Biological Sciences 16, no. 1: 37-49.