In the continuous cropping of Panax notoginseng, the pathogenic fungi in the rhizosphere soil increased and infected the roots of Panax notoginseng, resulting in a decrease in yield. This is an urgent problem that needs to be solved in order to effectively overcome the obstacles associated with the continuous cropping of Panax notoginseng. Previous studies have shown that Bacillus subtilis inhibits pathogenic fungi in the rhizosphere of Panax notoginseng, but the inhibitory effect was not stable. Therefore, we hope to introduce biochar to help Bacillus subtilis colonize in soil. In the experiment, fields planted with Panax notoginseng for 5 years were renovated, and biochar was mixed in at the same time. The applied amount of biochar was set to four levels (B0, 10 kg·hm⁻²; B1, 80 kg·hm⁻²; B2, 110 kg·hm⁻²; B3, 140 kg·hm⁻²), and Bacillus subtilis biological agent was set to three levels (C1, 10 kg·hm⁻²; C2, 15 kg·hm⁻²; C3, 25 kg·hm⁻²). The full combination experiment and a blank control group (CK) were used. The experimental results show that the overall Ascomycota decreased by 0.86%~65.68% at the phylum level. Basidiomycota increased by −73.81%~138.47%, and Mortierellomycota increased by −51.27%~403.20%. At the genus level, Mortierella increased by −10.29%~855.44%, Fusarium decreased by 35.02%~86.79%, and Ilyonectria increased by −93.60%~680.62%. Fusarium mainly causes acute bacterial wilt root rot, while Ilyonectria mainly causes yellow rot. Under different treatments, the Shannon index increased by −6.77%~62.18%, the Chao1 index increased by −12.07%~95.77%, the Simpson index increased by −7.31%~14.98%, and the ACE index increased by −11.75%~96.12%. The good_coverage indices were all above 0.99. The results of a random forest analysis indicated that Ilyonectria, Pyrenochaeta, and Xenopolyscytalum were the top three most important species in the soil, with MeanDecreaseGini values of 2.70, 2.50, and 2.45, respectively. Fusarium, the primary pathogen of Panax notoginseng, ranked fifth, and its MeanDecreaseGini value was 2.28. The experimental results showed that the B2C2 treatment had the best inhibitory effect on Fusarium, and the relative abundance of Fusarium in Panax notoginseng rhizosphere soil decreased by 86.79% under B2C2 treatment; the B1C2 treatment had the best inhibitory effect on Ilyonectria, and the relative abundance of Ilyonectria in the Panax notoginseng rhizosphere soil decreased by 93.60% under B1C2 treatment. Therefore, if we want to improve the soil with acute Ralstonia solanacearum root rot, we should use the B2C2 treatment to improve the soil environment; if we want to improve the soil with yellow rot disease, we should use the B1C2 treatment to improve the soil environment.

Vertical agriculture has developed rapidly in recent years, pushing artificial light planting to new heights. Under indoor artificial light, the light supply mode has practical significance in studying the impact of lighting conditions on plants. This experiment involved five intermittent light supply modes (with a light period of 16 h and a dark period of 8 h (16/8) as the control group, with a light period of 8 h and a dark period of 4 h repeated twice a day (8/4), a light period of 4 h and a dark period of 2 h repeated four times a day (4/2), a light period of 2 h and a dark period of 1 h repeated eight times a day (2/1), and a light period of 1 h and a dark period of 0.5 h repeated 16 times a day (1/0.5) (as the experimental groups). A total of ten treatments were combined before the continuous light supply mode (B) and after the continuous light supply mode (A). Through experimental settings, we explored the response mechanisms to intermittent and continuous light supply modes as they pertain to lettuce growth, nutrient content, photosynthetic parameters, and light stress. Through research, it was found that continuous light supply significantly increased plant height, root length, aboveground dry and fresh weight, and the underground dry and fresh weight of lettuce. The treatments with a light period 4 h/dark period 2 h (4/2) and a light period 8 h/dark period 4 h (8/4) significantly increased the N, P, K, and Cu contents. Additionally, continuous light supply helped stabilize the Mg, Ca, and Mn contents across all treatment groups. Stomatal closure has been found to cause a decrease in the rate of net photosynthesis, transpiration, and intercellular CO₂ concentration. The energy absorbed by antenna pigments is significantly increased when participating in photochemical reactions; however, continuous light supply has been observed to reduce the absorption flux per reaction center (ABS/RC), trapped energy flux per reaction center (TR₀/RC), electron transport flux per reaction center (ET₀/RC), and the probability that a trapped exciton moves an electron into the electron transport chain beyond QA⁻ (at t = 0) (ET₀/TR₀). Conversely, the electron transport flux per cross section (ET₀/CS) has been found to increase significantly. In summary, among the intermittent light supply treatment groups, the 2/1 treatment group showed the best response to growth indicators, nutrient absorption, and photosynthesis, and could improve the quality of lettuce without adding additional light energy. Continuous light supply in the short term can improve the growth and nutrient absorption of lettuce; both of the two light supply modes produced light stress on lettuce, and this light stress caused by non-circadian rhythm forced the lettuce to increase its photochemical quenching (qI) and electro transport flux crossover (ET₀/CS). This paper may provide a theoretical reference for the use of light supply modes in plant factories to improve vegetable yield, and for the study of the response mechanism of light stress under non-circadian rhythm.

The fungal community structure and soil fertility in rhizosphere soil have an important effect on the health of Panax notoginseng (P. notoginseng). The attack of pathogenic fungi and the imbalance of soil fertility can easily lead to diseases. The effect of Bacillus subtilis on improving the community structure of soil fungi has been confirmed, and the corresponding biological agent products have been commercialized. A pot experiment carried out in a greenhouse explored the effect of a biological agent and fertilizer on the fungal community in the rhizosphere of P. notoginseng. In the experiment, fertilization and the addition of biological agents were set up with three gradients, respectively, and the full coupling experiment was adopted, and the blank control group (CK) was set up at the same time. Therefore, there were thirteen treatments in the experiment. NH4 decreased between 36.42% and 11.56%, AP increased between 6.03% and 92.46%, AK increased between 2.99% and 25.40%, TN increased between 0.10% and 9.41%, and TP increased by 18.25% to 47.73% The addition of Bacillus subtilis biological agent decreased the Chao1, Shannon, Simpson, and ACE index of fungi in the rhizosphere soil of P. notoginseng. The Chao1 index decreased between 0.39% and 78.22%; the ACE index decreased between 0.43% and 78.24%. The main pathogenic fungi Cylindrocarpon and Fusarium of P. notoginseng were different in the experimental results. Cylindrocarpon decreased under F1C1, F2C1, and F3C2 treatments, while Fusarium increased under F1C1, F2C2, F3C1, and F3C3 treatments and decreased Fusarium content in rhizosphere soil of P. notoginseng in other treatments. RDA analysis (Redundancy analysis) showed that NH4-N was negatively correlated with the main pathogen Cylindrocarpon, Fusarium, and Ilyonectria, while AP and AK were positively correlated with Cylindrocarpon, Fusarium, and Ilyonectria. The results of the GRA-TOPSIS analysis showed that the score of F3C2 was the highest, while F2C3 and F2C1 ranked second and third, respectively. The calculation results of the theoretical model based on GRA-TOPSIS analysis showed that the GRA-TOPSIS score was highest when the theoretical optimal fertilizer application rate and bacteria application rate were 116.31 kg hm⁻² and 15.83 kg hm⁻², respectively.