Background: Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results: The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30–110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that Y_X/S and Y_L/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (Y_X/S: 0.414) and 3.20 g/L (Y_L/S: 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (Y_X/S: 0.534) and 3.08 g/L (Y_L/S: 0.139), respectively, with a lipid content of 26.02%. Conclusions: This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid production. Feasibilities on FW and FVW-derived VFAs were evaluated, and meaningful information was provided for practical applications.

Anaerobic digestion is one of the most sustainable and promising technologies for the management of organic residues. China plays an important role in the world’s biogas industry and has accumulated rich and valuable experience, both positive and negative. The country has established relatively complete laws, policies and a subsidy system; its world-renowned standard system guarantees the implementation of biogas projects. Its prefabricated biogas industry has been developed, and several biogas-linked agricultural models have been disseminated. Nonetheless, the subsidy system in China’s biogas industry is inflexible and cannot lead to marketization, unlike that of its European counterpart. Moreover, the equipment and technology levels of China’s biogas industry are still lagging and underdeveloped. Mono-digestion, rather than co-digestion, dominates the biogas industry. In addition, biogas upgrading technology is immature, and digestate lacks planning and management. China’s government subsidy is reconsidered in this work, resulting in the recommendation that subsidy should be based on products (i.e., output-oriented) instead of only input subsidy for construction. The policy could focus on the revival of abandoned biogas plants as well.

Previous studies showed that adding zero valent iron (ZVI) can increase the methane production and degradation rate of organic waste by improving the performance of anaerobic digester. However, our study firstly found that ZVI (37 μm, 10 g/L) inhibited the anaerobic digestion (AD) of cow manure and lignocellulose. ZVI significantly increased the methanogenic rate of cow manure in the first 6 days, but decreased the accumulative methane yield and volatile fatty acids yield by 10.3 and 12%, respectively. The effect of ZVI on AD of liquid biomass separated from cow manure was positive, but the effect on solid biomass was negative. These results indicated that ZVI enhanced the AD of easily biodegradable organics but inhibited the biodegradation of refractory organics (lignocellulose). By analyzing the varying effects of ZVI in diverse anaerobic systems, it was found that the effects were influenced by the characteristics of substrate and inoculum-substrate ratio. This study suggested that only proper ZVI addition can improve the AD process depending on the feeding materials.