This study aimed to evaluate the effectiveness of the experimental formulation containing chlamydospores of Duddingtonia flagrans and Pochonia chlamydosporia fungi, against Moniezia expansa. Two experiments were carried out. The first experiment evaluated the in vitro efficacy using 1 g of the experimental formulation (V1) added to 100 M. expansa eggs and the control (V2) (without the fungal formulation). Intact eggs or eggs with alterations were counted in order to evaluate their effectiveness. The second experiment evaluated the action of the fungal formulation on M. expansa eggs after passing through the gastrointestinal tract of goats. Three groups were identified as B1, B2, and B3, which received 1.0, 1.5 g of experimental fungal formulation, and placebo, respectively. In experiment 1, all the eggs in V1 were subjected to the predatory action of fungi, while in V2, the eggs remained intact. In experiment 2, the reduction of eggs in groups B1 and B2 were 49% and 57% 24 h after ingestion, 60% and 63% 48 h after, and 48% and 58% 72 h after. The predatory capacity against M. expansa eggs shown in the tests demonstrated that experimental fungal formulation has the potential to be used on integrated helminth control programs.

As verminoses gastrintestinais representam um dos principais entraves da caprinocultura, reduzindo o desempenho produtivo e comprometendo a sanidade dos rebanhos. O controle químico, amplamente utilizado, tem favorecido o surgimento de resistência parasitária as principais bases químicas disponíveis no mercado, levando à uma busca por métodos alternativos. Este estudo teve como objetivo, avaliar a eficácia in vitro do isolado Metarhizium robertsii (LCM S15) na concentração de 10 × 109 conídeos/mL, sobre larvas de nematoides gastrintestinais (NGIs) de caprinos, e avaliar, por microscopia eletrônica de varredura (MEV), aderência de conídios de Metarhizium robertsii (LCM S15) em formulação oleosa sobre o tegumento de larvas L3 de NGIs. O isolado apresentou redução de 71,17% das larvas e adesão de conídios ao tegumento observada por microscopia eletrônica de varredura, evidenciando potencial do isolado no controle biológico de NGIs de caprinos.

The rise in anthelmintic resistance in small ruminants has driven the search for sustainable control alternatives. Among these, entomopathogenic fungi such as Metarhizium robertsii stand out for their potential to reduce the free-living stages of gastrointestinal nematodes (GINs). This study evaluated the field efficacy of M. robertsii (LCM S15) under climatic conditions in the Recôncavo region of Bahia, Brazil. The experiment was conducted between November 2022 and July 2023 in a completely randomized design with four groups (n = 8): aqueous control, oil control, aqueous suspension, and oil formulation of M. robertsii. Egg counts per gram of feces (EPG) and L3 larval recovery were assessed by coproculture and the Baermann technique. Efficacy ranged from 15.23% to 27.34%, with the oil formulation showing higher performance. Haemonchus sp. and Trichostrongylus sp. were the most prevalent genera. These findings suggest the potential of M. robertsii (LCM S15) as a biological control agent under field conditions.