Diabetic Macular Edema (DME) represents a significant visual impairment among individuals with diabetes, leading to a dramatic reduction in visual acuity and potentially resulting in irreversible vision loss. Optical Coherence Tomography (OCT), a technique that produces high-resolution retinal images, plays a vital role in the clinical assessment of this condition. Physicians typically rely on OCT B-Scan images to evaluate DME severity. However, manual interpretation of these images is susceptible to errors, which can lead to detrimental consequences, such as misdiagnosis and improper treatment strategies. Hence, there is a critical need for more reliable diagnostic methods. This study aims to address this gap by proposing an automated model based on Generative Adversarial Networks (GANs) to generate OCT B-Scan images of DME. The model synthesizes images from patients' baseline OCT B-Scan images, which could potentially enhance the robustness of DME detection systems. We employ five distinct GANs in this study: Deep Convolutional GAN, Conditional GAN, CycleGAN, StyleGAN2, and StyleGAN3, drawing comparisons across their performance. Subsequently, the hyperparameters of the best-performing GAN are fine-tuned using Particle Swarm Optimization (PSO) to produce more realistic OCT images. This comparative analysis not only serves to improve the detection of DME severity using OCT images but also provides insights into the appropriate choice of GANs for the effective generation of realistic OCT images from the baseline OCT datasets.

Diabetic Macular Edema (DME) is a severe ocular complication commonly found in patients with diabetes. The condition can precipitate a significant drop in VA and, in extreme cases, may result in irreversible vision loss. Optical Coherence Tomography (OCT), a technique that yields high-resolution retinal images, is often employed by clinicians to assess the extent of DME in patients. However, the manual interpretation of OCT B-scan images for DME identification and severity grading can be error-prone, with false negatives potentially resulting in serious repercussions. In this paper, we investigate an Artificial Intelligence (AI) driven system that offers an end-to-end automated model, designed to accurately determine DME severity using OCT B-Scan images. This model operates by extracting specific biomarkers such as Disorganization of Retinal Inner Layers (DRIL), Hyper Reflective Foci (HRF), and cystoids from the OCT image, which are then utilized to ascertain DME severity. The rules guiding the fuzzy logic engine are derived from contemporary research in the field of DME and its association with various biomarkers evident in the OCT image. The proposed model demonstrates high efficacy, identifying images with DRIL with 93.3% accuracy and successfully segmenting HRF and cystoids from OCT images with dice similarity coefficients of 91.30% and 95.07% respectively. This study presents a comprehensive system capable of accurately grading DME severity using OCT B-scan images, serving as a potentially invaluable tool in the clinical assessment and treatment of DME.

Background/Objectives: Disorganization of the retinal inner layers (DRIL) is an important biomarker of diabetic macular edema (DME) that has a very strong association with visual acuity (VA) in patients. But the unavailability of annotated training data from experts severely limits the adaptability of models pretrained on real-world images owing to significant variations in the domain, posing two primary challenges for the design of efficient computerized DRIL detection methods. Methods: In an attempt to address these challenges, we propose a novel, self-supervision-based learning framework that employs a huge unlabeled optical coherence tomography (OCT) dataset to learn and detect clinically applicable interpretations before fine-tuning with a small proprietary dataset of annotated OCT images. In this research, we introduce a spatial Bootstrap Your Own Latent (BYOL) with a hybrid spatial aware loss function aimed to capture anatomical representations from unlabeled OCT dataset of 108,309 images that cover various retinal abnormalities, and then adapt the learned interpretations for DRIL classification employing 823 annotated OCT images. Results: With an accuracy of 99.39%, the proposed two-stage approach substantially exceeds the direct transfer learning models pretrained on ImageNet. Conclusions: The findings demonstrate the efficacy of domain-specific self-supervised learning for rare retinal pathological detection tasks with limited annotated data.

Aligning protein sequences with cryo-EM density maps remains challenging due to limited paired data, structural heterogeneity, varying map resolutions, and the presence of multiple conformational states. In this work, we propose a multimodal representation learning framework that learns a shared latent space between protein sequences and cryo-EM density maps for cross-modal retrieval. Our approach combines pretrained protein sequence embeddings with a volumetric cryo-EM encoder trained using self-supervised representation learning and transfer learning. The resulting model enables bidirectional retrieval between sequences and density maps while learning biologically meaningful structural representations. Experimental results demonstrate strong retrieval performance across both sequence-to-map and map-to-sequence tasks, achieving median retrieval ranks of 2–3 within a database of 3,275 cryo-EM maps. The learned embedding space shows a clear separation between matched and unmatched sequence–map pairs and remains robust across varying cryo-EM resolutions. Additionally, the model generalizes across species, successfully retrieving conserved mouse protein structures using human sequence embeddings. Our findings demonstrate that joint latent-space learning provides a promising direction for connecting protein sequences with cryo-EM structural representations, with potential applications in structural retrieval, protein annotation, and multimodal biological representation learning.