Due to the diversity of Echinochloa species and the limited understanding of their damage processes in rice fields, clarifying the biological properties of distinct species could help create a foundation for effective control techniques. Pot experiments and field competition trials were conducted using eight Echinochloa species to elucidate their biological differences and assess their varying levels of negative impact on rice. The survey outcomes showed that E. oryzoides had the highest 1000-grain weight (3.12 g) while E. colona had the lowest (0.90 g). The largest grain number per spikelet found in E. glabrescens (940) was 3.4 times greater than that in E. oryzoides (277). Different species responded variably to changes in temperature and photoperiod. Except for E. caudate, all Echinochloa species exhibited a shortened growth period with the delay of the sowing date. Under field competitive conditions, all Echinochloa species exhibited significantly greater net photosynthetic rates than rice, with E. crusgalli exhibiting the highest photosynthetic capacity. Moreover, in this resource-limited setting, barnyardgrass species had a decrease in tiller formation and panicle initiation but a significant increase in plant height. These findings contribute valuable insights into the biological characteristics of barnyardgrass populations and provide guidance for implementing effective control measures in rice fields.