Climate change and human activities have significantly altered the soil redistribution processes in the Panama Canal Watershed, affecting Lakes Alajuela, Gatún, and Miraflores, which are essential for the functioning and operation of the Canal and the supply of drinking water. Since 2018, the Technological University of Panama has been researching the micro-watershed of La Zanguenga Creek using advanced nuclear techniques to quantify the volume of mobilized soil, as well as to identify the possible sources from which the soil originates, with the purpose of monitoring the effects of erosive processes mainly caused by the region’s intense rainfall. This micro-watershed is located in the corregimiento of Herrera, district of La Chorrera, province of Panamá Oeste; and in turn, is part of the Caño Quebrado River sub-watershed, which discharges its waters into Gatún Lake, which is part of the Panama Canal watershed. La Zanguenga Creek contributes an estimated solid flow of 112,89 tons per hectare per year, which is an indicator of the degree of erosion in the region. A total of 88 samples were taken at 31 strategic points, allowing for the estimation of the volume of displaced soil and the origin of the sediments. The results show considerable soil losses ranging between 15,70 to 21,51 tons/ha/year in secondary mixed broadleaf forest, 1,83 to 5,25 tons/ha/year in pineapple crops, and 0,20 to 0,23 tons/ha/year in pasture. The main sources of sediment identified were pineapple cultivation, pastures, secondary mixed broadleaf forests, and fallow lands.