In the Brazilian Atlantic Rainforest (AF), amphibians (625 species) face habitat degradation leading to stressful thermal conditions that constrain animal activity (e.g., foraging and reproduction). Data on thermal ecology for these species are still scarce. We tested the hypothesis that environmental occupation affects the thermal tolerance of amphibian species more than their phylogenetic relationships. We evaluated patterns of thermal tolerance of 47 amphibian species by assessing critical thermal maxima and warming tolerances, relating these variables with ecological covariates (e.g., adult macro‐ and microhabitat and site of larval development). We used mean and maximum environmental temperature, ecological covariates, and morphological measurements in the phylogenetic generalized least squares model selection to evaluate which traits better predict thermal tolerance. We did not recover phylogenetic signal under a Brownian model; our results point to a strong association between critical thermal maxima and habitat and development site. Forest species were less tolerant to warm temperatures than open area or generalist species. Species with larvae that develop in lentic environment were more tolerant than those in lotic ones. Thus, species inhabiting forest microclimates are more vulnerable to the synergistic effect of habitat loss and climate change. We use radar charts as a quick evaluation tool for thermal risk diagnoses using aspects of natural history as axes.

Realistic predictions about the impacts of climate change onbiodiversity requires gathering ecophysiological data and the critical thermal maxima (CTMax) is the most frequently used index to assess the thermal vulnerability of species. In the present study, we performed a systematic review to understand how acclimation and altitude affect CTMax estimates for amphibian and non-avian reptile species. We retrieved CTMax data for anurans, salamanders, lizards, snakes, and turtles/terrapins. Data allowed to perform a multilevel random effects meta-analysis to answer how acclimation temperature affect CTMax of Anura, Caudata, and Squamata and also meta-regressions to assess the influence of altitude on CTMax of frogs and lizards. Acclimation temperature influenced CTMax estimates of tadpoles, adult anurans, salamanders, and lizards, but not of froglets. In general, the increase in acclimation temperature led to higher CTMax values. Altitudinal bioclimatic gradient had an inverse effect for estimating the CTMax of lizards and anuran amphibians. For lizards, CTMax was positively influenced by the mean temperature of the wettest quarter. For anurans, the relationship is inverse; we recover a trend of decreasing CTMax when max temperature of warmest month and precipitation seasonality increase. There is an urgent need for studies to investigate the thermal tolerance of subsampled groups or even for which we do not have any information such as Gymnophiona, Serpentes, Amphisbaena, and Testudines. Broader phylogenetic coverage is mandatory for more accurate analyses of macroecological and evolutionary patterns for thermal tolerance indices as CTMax.

In the present study we used larval amphibians (Rhinella jimi) as prey, and beetle larvae (Hydrophilus sp.) as predator to assess if increased water temperature influences predator-prey dynamics. The experiment was carried out in replicated aquatic microcosms with two different water temperatures (control and a ‘warming water’ scenario). Survival of tadpoles decreased significantly in the treatment compared to the control and at the end of the experiment, all tadpoles in the ‘warming water’ scenario had been ingested, unlike in the control treatment, where only half of the tadpoles were ingested. Our results demonstrate that the alterations in abiotic conditions (water temperature) can reshape predator-prey dynamics in a future scenario of global warming. The consequent increase of predation may intensify negative effects on population size in anuran larvae. Although changes are likely, they are complex and often counterintuitive.

Salmonella spp. are known to persist in the environment. Wild animals are believed to act as important reservoirs, with antimicrobial resistance frequently occurring in the environment. However, little is known about the role of the wildlife in Bahia as a reservoir for Salmonella in Brazil. This study aimed to isolate and characterize Salmonella spp. from wildlife in the Atlantic Forest and Caatinga biomes considering indicators such as the animal species, degree of anthropization, sampling area, and feeding habits. Convenience wildlife sampling and characterization were conducted, followed by microbiological and molecular identification of Salmonella isolates, serotyping, and antimicrobial susceptibility testing. A total of 674 fecal samples were collected from 12 municipalities during 2015–2021, and 4 were positive for the following Salmonella species: Salmonella enterica subspecies enterica serovar Agona (n = 1), Salmonella enterica subsp. enterica serogroup O:16 (n = 2), and Salmonella enterica subsp. enterica serovar Muenchen (n = 1). Antimicrobial susceptibility analysis revealed that one isolate was resistant to six antibiotics, including extended-spectrum penicillins and beta-lactamase inhibitors. These results indicated a low frequency of Salmonella spp. in the sampled forest fragments. The presence of Salmonella in wild animals increases the risk to public health and biodiversity and indicates that they can act as sentinels of environmental contamination or indicators of preservation.