From the ancestral bisexual flower (BC model) with radial symmetry to Fabaceae flower with bilateral symmetry and a keel petal, it is critical to understand ABC model because high seed yield and food safety depend on flower bud development. Thus, we summarize the information of the genetic mechanism that explains the identity of the floral organs for Sophoreae, Phaseoleae, Dalbergieae and Genisteae tribes. Moreover, we examine the role of non-coding RNAs on floral development at Cajaninae subtribe, Dalbergieae and Genisteae tribes.

La esparteína es un alcaloide con actividad bacteriostática sobre el género Mycobacterium. El objetivo de este trabajo fue evaluar la acción antimicrobiana de la esparteína en el crecimiento de cuatro cepas ATCC de Mycobacterium tuberculosis (susceptible, resistente a isoniazida, resistente a rifampicina y multidrogorresistente) in vitro. La evaluación de la actividad bactericida del sulfato de esparteína se realizó a través de una adaptación del método de ensayo de cultivo y susceptibilidad a medicamentos antituberculosos mediante observación microscópica (MODS, por sus siglas en inglés), según el protocolo descrito en el manual técnico elaborado por el Instituto Nacional de Salud. Los resultados demuestran que a concentraciones de 25; 50 y 100 mM de sulfato de esparteína, no se desarrollan unidades formadoras de colonia en las cuatro cepas evaluadas de Mycobacterium tuberculosis. Los resultados demuestran el potencial efecto antimicrobiano in vitro de la esparteína en la tuberculosis multidrogorresistente.

Carica papaya L. or “papaya” is a very important species in which many technological packages have been implemented, such as fertilization and inoculation with arbuscular mycorrhizal fungi (AMF). However, no studies have been conducted to evaluate the interaction between phosphorous (P) fertilization and AMF in in vitro-propagated papaya plantlets after the acclimatization phase. This study aimed to evaluate the effect of Rhizophagus irregularis and P on C. papaya growth. Papaya plants were propagated in vitro and received the treatments after taking root and being acclimatized for 15 days. The plants were inoculated with 0, 2, and 5 grams of R. irregularis and fertilized with 0, 30, and 60 ppm of P. Growth was evaluated after 45 days. All the analyzed shoot variables showed maximum growth when the plants were treated with 5 g of AMF and 60 ppm of P. Likewise, there was a significant increase in the number of leaves and leaf area index. In the root, the increase was higher in the fresh weight, but no statistical differences were found in its length. These results indicate that AMF and P are responsible for the increase in growth. Furthermore, the relatively high correlation between the percentage of mycorrhization and the other variables was not affected by the concentrations of P applied. The use of R. irregularis and P significantly increased the growth of C. papaya L. after the acclimatization phase.

Lupinus mutabilis is a legume with high agronomic potential and available transcriptomic data for which lncRNAs have not been studied. Therefore, our objective was to identify, characterize, and validate the drought-responsive lncRNAs in L. mutabilis. To achieve this, we used a multilevel approach based on lncRNA prediction, annotation, subcellular location, thermodynamic characterization, structural conservation, and validation. Thus, 590 lncRNAs were identified by at least two algorithms of lncRNA identification. Annotation with the PLncDB database showed 571 lncRNAs unique to tarwi and 19 lncRNAs with homology in 28 botanical families including Solanaceae (19), Fabaceae (17), Brassicaceae (17), Rutaceae (17), Rosaceae (16), and Malvaceae (16), among others. In total, 12 lncRNAs had homology in more than 40 species. A total of 67% of lncRNAs were located in the cytoplasm and 33% in exosomes. Thermodynamic characterization of S03 showed a stable secondary structure with −105.67 kcal/mol. This structure included three regions, with a multibranch loop containing a hairpin with a SECIS-like element. Evaluation of the structural conservation by CROSSalign revealed partial similarities between L. mutabilis (S03) and S. lycopersicum (Solyc04r022210.1). RT-PCR validation demonstrated that S03 was upregulated in a drought-tolerant accession of L. mutabilis. Finally, these results highlighted the importance of lncRNAs in tarwi improvement under drought conditions.

From Rhizosphere to Crop Production: Evolutionary and Molecular Insights into Plant Growth Promoting Rhizobacteria (PGPR)