Moroccan populations of Alnus glutinosa (L.) Gaerth. (Betulaceae) are found at the southern limit of the species’ range and are represented by tetraploid cytotypes with no available pollen data. The objective of this study was to assess the morphological and morphometric variability of the pollen, specifically focusing on pollen diameters and the number of apertures. To achieve this, we sampled 11 populations that are representative of the Moroccan distribution area of this species. We employed a hierarchical sampling design (11 populations, 10 trees per population, and from 30 to 300 pollen grains per tree, depending on the character measured) to examine different levels of variability: interpopulation, intrapopulation, and intraindividual. The results demonstrate that there is no discernible difference in the morphology or size of the pollen among the Moroccan tetraploid populations. However, we observed a high degree of intraspecific variability in pollen morphometric traits, but most of this variability is associated with the intraindividual level.