We have investigated with high resolution the timing of retinal precursor cell commitment to specific differentiated fates, using an in ovo modification of the in vitro "window-labeling" technique (A. M. Repka and R. Adler, J. Histochem. Cytochem. 40, 947-953, 1992a). The method involves an initial injection of tritiated thymidine into chick embryos, followed a specific number of hours later by an injection of bromodeoxyuridine (BrDU); cells born during this period are identified by being labeled with thymidine but not with BrDU. We used this method to determine, in a narrow region adjacent to the choroid fissure, the fate of cells born during defined 5-hr intervals between Embryonic Days (ED) 4-8. All the cohorts gave rise to heterogenous differentiated populations, indicating that time of cell birth is not a major cell fate determinant. A progressive restriction in the developmental potential of precursor cells, however, was suggested by the observed decrease in the number of different populations generated during each 5-hr period from ED 4 to 8, and supported also by dissociated cell culture experiments investigating the fate of cells born at different developmental stages. Microenvironmental influences were tested in vitro using cells windowed-labeled in ovo for 5 hr on ED 5. After spending at least 72 hr within the retina before their isolation for culture, these cells mimicked their in vivo fate, giving rise predominantly to nonphotoreceptor neurons; a completely different behavior was observed when the cells were isolated after shorter exposures to the retinal microenvironment, when they gave rise predominantly to photoreceptors. Together with data demonstrating that differential cell death cannot account for these results, our results are consistent with the hypothesis that cell fate determination occurs after the time of terminal mitosis.

To gain insight into what differences might restrict the capacity for limb regeneration in Xenopus froglets, we used High Performance Liquid Chromatography (HPLC)/double mass spectrometry to characterize protein expression during fibroblastema formation in the amputated froglet hindlimb, and compared the results to those obtained previously for blastema formation in the axolotl limb.

Aims/hypothesis: The loss of pericytes surrounding the retinal vasculature in early diabetic retinopathy underlies changes to the neurovascular unit that lead to more destructive forms of the disease. However, it is unclear which changes lead to loss of retinal pericytes. This study investigated the hypothesis that chronic increases in one or more inflammatory factors mitigate the signalling pathways needed for pericyte survival. Methods: Loss of pericytes and levels of inflammatory markers at the mRNA and protein levels were investigated in two genetic models of diabetes, Ins2^Akita/+ (a model of type 1 diabetes) and Lepr^db/db (a model of type 2 diabetes), at early stages of diabetic retinopathy. In addition, changes that accompany gliosis and the retinal vasculature were determined. Finally, changes in retinal pericytes chronically incubated with vehicle or increasing amounts of IFNγ were investigated to determine the effects on pericyte survival. The numbers of pericytes, microglia, astrocytes and endothelial cells in retinal flatmounts were determined by immunofluorescence. Protein and mRNA levels of inflammatory factors were determined using multiplex ELISAs and quantitative reverse transcription PCR (qRT-PCR). The effects of IFNγ on the murine retinal pericyte survival-related platelet-derived growth factor receptor β (PDGFRβ) signalling pathway were investigated by western blot analysis. Finally, the levels of cell death-associated protein kinase C isoform delta (PKCδ) and cleaved caspase 3 (CC3) in pericytes were determined by western blot analysis and immunocytochemistry. Results: The essential findings of this study were that both type 1 and 2 diabetes were accompanied by a similar progression of retinal pericyte loss, as well as gliosis. However, inflammatory factor expression was dissimilar in the two models of diabetes, with peak expression occurring at different ages for each model. Retinal vascular changes were more severe in the type 2 diabetes model. Chronic incubation of murine retinal pericytes with IFNγ decreased PDGFRβ signalling and increased the levels of active PKCδ and CC3. Conclusions/interpretation: We conclude that retinal inflammation is involved in and sustains pericyte loss as diabetic retinopathy progresses. Moreover, IFNγ plays a critical role in reducing pericyte survival in the retina by reducing activation of the PDGFRβ signalling pathway and increasing PKCδ levels and pericyte apoptosis. Graphical

We have investigated whether the activin family of growth factors is involved in the regulation of retinal cell differentiation. Immunocytochemistry and in situ hybridization have shown that activin/inhibin subunits alpha, betaA, and betaB; receptors II and IIB; follistatin; and a follistatin-like gene are expressed in different regions of the chick embryo retina in developmentally regulated patterns. When tested in dissociated retinal cultures, activin did not appear to affect cell survival or proliferation, but it exerted marked inhibitory effects on the differentiation of photoreceptors, while stimulating the differentiation of nonphotoreceptor neurons; both effects were concentration-dependent and follistatin-sensitive. The results are consistent with the possibility that activin family members play significant roles in the regulation of retinal development.