12-week supplementation with the new probiotic formulation containing Lactiplantibacillus plantarum 299v, Saccharomyces cerevisiae var. boulardii , and octacosanol showed beneficial effects on certain metabolic parameters, proinflammatory proteins and appetite-regulating hormones in obese women.

Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.

A variety of specific plant oils produced from plants other than sunflowers and olives has been offered on the food market, most of them obtained using cold pressing in order to preserve sensitive oil components. The objective of this study was to evaluate their fatty acid (FA) compositions by means of nutritional quality indices. FA profiles (37 FAs) of 20 commercially available specific edible plant oils were obtained using GC-FID and further evaluated by calculating lipid quality indices. FA profiles and their corresponding quality indices showed the expected variability, depending on the plant source. For the purpose of comparison, the same indices were determined for sunflower and olive oil: the polyunsaturated-to-saturated FA ratio (PUFA/SFA) was 5.1 and 0.5, the hypocholesterolemic/hypercholesterolemic ratio (HH) was 13.0 and 6.8, the index of atherogenicity (IA) was 0.08 and 0.14, the index of thrombogenicity (IT) was 0.23 and 0.24, and the unsaturation index (UI) was 146.6 and 93.5, respectively. A higher PUFA/SFA ratio is beneficial for cardiovascular health, as are a lower IA and IT. The UI indicates stability of unsaturated FAs during storage and processing. Flaxseed oil was the only one showing a PUFA/SFA ratio higher than sunflower oil (5.8). Regarding IA, flaxseed, almond, apricot, plum, hazelnut, macadamia, and sea buckthorn oils were similar to sunflower oil; sesame, black cumin, poppy, pumpkin, avocado, raspberry seed, argan, moringa, and rose seed oils resembled olive oil; and palm oil was isolated at 0.80, while coconut oil reached 23.4. Flaxseed, almond, apricot, plum, raspberry seed, macadamia, rose seed, and sea buckthorn oils showed a lower IT than sunflower and olive oils (range 0.06–0.18). Coconut and palm oils showed lower HH ratios than olive oil, whereas, in the case of flaxseed, almond, apricot, plum, hazelnut, and sea buckthorn oils, this ratio was higher than the one in sunflower oil (range 14.0–16.1). Flaxseed oil was characterized by the highest UI (208.4), while others were distributed along the 90–170 interval (except coconut and palm oils). According to their nutritional quality indices, a variety of plant oils are valuable sources of FAs in human nutrition.

Obesity is considered to be a chronic complex disease that increases the risk of diabetes, heart diseases and certain cancers. According to the literature, in obese people the gastrointestinal microbiota is disturbed, which could be the cause of the onset of obesity and related diseases. Moreover, there are small non-coding RNAs (microRNAs) that are disturbed in obesity, which are also considered to be a possible mechanism of probiotics' action. Certain microRNAs are involved in the development and metabolism of adipose tissue cells, as well as the secretion and action of insulin, showing that changed expression of certain miRNAs could have a significant impact on the onset and development of obesity and obesity-related diseases. Thus, miRNAs are considered to be possible markers for the diagnosis and prognosis of various metabolic diseases, and possible therapeutic targets for the treatment of obesity and related diseases. Considering the growing need of the healthcare system for nutraceuticals and dietary supplements that present effective and safe medical nutritive therapy in obese individuals, this work aimed to assess the beneficial effects of probiotics on obesity-related microRNAs. In this review, we described the role of microRNAs and probiotics in obesity and the association between probiotics and obesityrelated microRNAs. Circulating miRNAs' profile in obese individuals significantly differs from that of normal-weight individuals. miRNAs such as miR-155, miR-221, miR-24-3p, and miR181a are over-expressed, while miR-26b and 125a are under-expressed in obese patients compared to non-obese individuals. It has been found that supplementation with Bifidobacterium bifidum and Lactobacillus acidophilus could downregulate the expression of miR-155 and miR221, and upregulate the expression of miR-26b. Supplementation with a probiotic formulation containing S. boulardii, L. plantarum 299v, and octacosanol led to the downregulation of miR155 and miR-24-3p expression. miR-125a and miR-181a were upregulated and downregulated, respectively, after the intervention with L. delbrueckii and L. rhamnosus. After reviewing the available literature, we can conclude that probiotics have beneficial effects on microRNAs altered in obesity, which could provide an effective strategy for the management of obesity.