Accurate and early diagnosis of bean common mosaic virus (BCMV) in Phaseolus vulgaris tissues is critical since the pathogen can spread easily and have long-term detrimental effects on bean production. The use of resistant varieties is a key factor in the management activities of BCMV. The study reported here describes the development and application of a novel SYBR Green-based quantitative real-time PCR (qRT-PCR) assay targeting the coat protein gene to determine the host sensitivity to the specific NL-4 strain of BCMV. The technique showed high specificity, validated by melting curve analysis, without cross-reaction. Further, the symptoms development of twenty advanced common bean genotypes after mechanical BCMV-NL-4 infection was evaluated and compared. The results showed that common bean genotypes exhibit varying levels of host susceptibility to this BCMV strain. The YLV-14 and BRS-22 genotypes were determined as the most resistant and susceptible genotypes, respectively, in terms of aggressiveness of symptoms. The accumulation of BCMV was analyzed in the resistant and susceptible genotypes 3, 6, and 9 days following the inoculation by the newly developed qRT-PCR. The mean cycle threshold (Ct) values showed that the viral titer was significantly lower in YLV-14, which was evident in both root and leaf 3 days after the inoculation. The qRT-PCR thus facilitated an accurate, specific, and feasible assessment of BCMV accumulation in bean tissues even in low virus titers, allowing novel clues in selecting resistant genotypes in the early stages of infection, which is critical for disease management. To the best of our knowledge, this is the first study of a successfully performed qRT-PCR to estimate BCMV quantification.

Coriander (Coriandrum sativum L.), one of the oldest essential oil-bearing spice and medicinal plants in the world, is widely used in folk medicine and seasoning in food. Thanks to its phytochemicals that have various bioactivity, recently, lots of studies have been conducted on coriander breeding. Classical breeding studies led to the development of new varieties over time. These studies could not accelerate the improvements in some of the plant parameters such as essential oil yield, biotic-abiotic stress resistance, and some other quality characteristics. In this respect, it is very important to conduct basic research on modern breeding programs to determine the yield and quality performance of coriander. Although coriander cultivation is carried out in different parts of the world today, genetic studies on coriander breeding are limited. Studies conducted with genome sequencing technology and molecular markers, the genetic diversity and population structure of the coriander were investigated, and a certain amount of progress was obtained for coriander breeding. This review aims to highlight the importance of coriander biotechnological advancements for coriander breeding. In the light of this information, biotechnological solutions can be enhanced coriander breeding. The review will provide more practical information for coriander researchers.