Sexual reproduction through seeds is an effective way to renew plant populations and increase their genetic diversity, but seed germination process is complicated and relatively difficult due to the restriction of environmental conditions. Wetland plants that reproduce sexually through seeds may be affected by changes in moisture and temperature. This study aims to explore the ecological adaptation strategies of seed germination of Lilium concolor var. megalanthum under different hydrothermal conditions. Controlled experiments were conducted to investigate the germination performance of L. concolor var. megalanthum seeds at different temperatures (10°C, 15°C, 20°C, 25°C, and 30°C) and simulated drought stress conditions using PEG-6000 solutions (0%, 5%, 10%, 15%, and 20%). The results showed that temperature, drought stress, and their interaction significantly affected the days to first germination, germination percentage, coefficient of germination rate, germination energy, germination index, and vigor index of seeds (p<0.01). The germination percentage, germination index, and vigor index of seed were significantly higher at 25°C compared to other temperatures (p<0.01). The interaction between low temperature and drought stress significantly delayed the days to first germination. The inhibition of drought stress on seed germination was enhanced by PEG-6000 solution under high temperature. Under the conditions of 25°C and 5% PEG-6000 solution concentration, seeds of L. concolor var. megalanthum exhibited optimal germination parameters. At 10°C and 15°C, the seeds exhibited the highest tolerance to PEG-6000-simulated drought stress. Rehydration germination results showed that extreme temperatures and drought stress conditions inhibit seed germination of L. concolor var. megalanthum without damaging seed structure. The germination pattern of seeds under variable temperature and drought stress conditions reflects their adaptive strategies developed over long-term evolution to cope with the environmental conditions.

Arbuscular mycorrhizal fungi (AMF) colonize the rhizosphere of plants and form a symbiotic association with plants. Mycorrhizal symbionts have diversified ecological roles and functions which are affected by soil conditions. Understanding the effects of different AMF inoculation on plants under varied nutritional conditions is of great significance for further understanding the effects of the external environment regulating mycorrhizal symbiosis on plant phenotypic traits. In this study, the effects of four AMF inoculation treatments on the growth and reproductive performance of cherry tomato (Solanum lycopersicum var. cerasiforme) were investigated under three nutrient levels by pot experiment. It was found that the growth-promoting effect of AMF on cherry tomato decreased with nutrient reduction, and the effects of the same AMF inoculation treatment on cherry tomato were different at different nutrient levels. Nutrient levels and AMF had interactive effects on flower characteristics, fruit yield, resource allocation, and seed germination of the cherry tomato. In addition, AMF could promote sexual reproductive investment. Nutrient levels and AMF also affected the accumulation of nitrogen and phosphorus in cherry tomato, and there were significant differences among different AMF inoculation treatments. The results indicated that nutrient differences could affect the symbiosis between AMF and plants, and confirmed that there were differences in the effects of the four AMF inoculation treatments on the growth and reproductive traits of plants. The differences in growth and reproduction characteristics of cherry tomato between different AMF inoculation treatments at different nutrient levels indicated that the effects of AMF mycorrhizal on the traits of cherry tomato were regulated by nutrients.

Scientists were concerned with the division of labor research early, and much current research in clonal plant ecology has proved environmentally induced or a developmentally programmed division of labor. However, the sexually dependent division of labor in clonal plants has received little attention, and few experimental tests have been conducted to date. Here, using reproductive ramets and non‐reproductive ramets in the clonal plant Iris laevigata, we test this hypothesis on morphology and physiology through field and manipulative experiments, including an isotope (¹⁵N) labeling method. With regard to morphology, under natural conditions, non‐reproductive ramets of I. laevigata were more abundant, taller, and consisted exclusively of leaves, while the relatively rare, smaller reproductive ramets consisted of leaves, flowers, or seeds. However, the mean biomass of non‐reproductive ramets was nearly half that of reproductive ramets. With regard to physiology, non‐reproductive ramets had a significantly higher net photosynthetic rate over a 24‐h period than reproductive ramets. Furthermore, the reproductive ramets stopped leaf growth when flower buds appeared in late May and lost photosynthetic capacity before late June, while its growing fruits still required a constant supply of many nutrients. Manipulative experiments showed that the non‐reproductive ramets supplied the nutrient demands for reproduction of the reproductive ramets through clonal integration as high as δ¹⁵N of the value (196.1 ± 73.39) of the seed. Thereby providing the first clear evidence of sexually dependent division of labor in clonal plants: Certain individuals constitute a very small amount of reproductive castes (reproductive ramets), whereas a large amount of other individuals (non‐reproductive ramets) are “workers” as in social insect societies whose role is primarily as producers. The rapid withering of reproductive ramets that failed to be pollinated highlights their role as irreversibly focused on reproduction, and those unable to reproduce do not contribute to the colony. Additionally, “producer” ramets positively influenced reproductive castes growth, and the division of labor was assumed to underlie the resource allocation pattern among ramets.

Introduction: The biomass and nutrient allocation strategies in plants are fundamental for predicting carbon storage and mineral and nutrient cycles in terrestrial ecosystems. However, our knowledge regarding the effects of multiple environmental factors on biomass and nutrient allocation remains limited.Methods: Here we manipulated soil composition (three levels), arbuscular mycorrhizal fungi inoculation (AMF, five levels), and root-knot nematode inoculation (RKN, two levels) using random block design to reveal the effects of these factors on biomass and nutrient allocation strategies of cherry tomato.Results and Discussion: Our results showed that biomass and nutrient allocation were affected by soil composition, AMF and RKN individually or interactively. The biomass and nutrient allocation in cherry tomato shows different adaptation strategies responded to the joint action of three factors. The reduction of soil nutrients increased belowground biomass allocation, and aboveground nitrogen and phosphorus concentration. AMF colonization increased aboveground biomass allocation and reproductive investment and promoted aboveground nitrogen and phosphorus inputs. Cherry tomato can mitigate the stress of RKN infection by investing more biomass and nutrients into belowground organs. Our study showed that plants can adjust their survival strategies by changing biomass and nutrient allocation to adapt to variation in soil abiotic and biotic factors. These findings contribute to our understanding of the adaptive processes of plant biomass and nutrient allocation strategies under multiple environmental factors.

Approximately 80 % of angiosperm species produce hermaphroditic flowers, which face the problem of male–male sexual interference (one or more anthers gets in the way of disseminating pollen from other anthers) or male–female sexual interference (the pistil interferes with disseminating pollen from the anthers by preventing the anther from touching a pollinator, or the anther prevents pollinator from depositing outcross pollen on the stigma). Slow stamen movement in hermaphrodite flowers has been interpreted as an adaptation for reducing male–male sexual interference. Using slow stamen movement in Lychnis cognata (Caryophyllaceae), this study presents new evidence that this phenomenon can reduce both male–male and male–female sexual interference. Ten stamens in L. cognata flowers vertically elongated their filaments in two batches and displayed similar patterns in pollen dispensing. More importantly, 10 stamens bend out of the floral centre by curving the filament also in 2 batches and pollen grains located at the flower centre displayed the highest viability. Thus, three stages of stamen movement can be identified, comprising two male stages (M1 and M2) and one female stage (F). We found that the main pollinator for L. cognata, Bhutanitis yulongensis (Papilionodae) generally preferred M1 flowers. Manipulation experiments show that vertical stamen movement enabled the anthers to dehisce at different times to prolong the presentation of pollen grains. Horizontal movement of the stamen decreased both male–male and male–female interference. However, vertical stamen movement had a minor role in increasing amount of pollen received by the stigma. This study provides the first direct experimental evidence of concurrent male–male and male–female interference in a flower. We suggest that the selection pressure to reduce such interference might be a strong force in floral evolution. We also propose that other selective pressure, including pollen dispensing mechanisms, pollen longevity, pollinator behaviour and weather, might contribute to floral evolution.