Common basil is well known for its aromatic and medicinal properties. This study was aimed to investigate effects of light spectrum on essential oil composition, antiradical activity and total phenolic and anthocyanin contents of green and purple varieties of Ocimum basilicum. Plants were placed under different spectral light compositions including red (R), blue (B), white (W), 50 % red+50 % blue (50:50 % R:B) and 70 % red+30 % blue (70:30 % R:B) provided by LED modules with same light intensity for a duration of 30 days. Antiradical activity, total phenolic and anthocyanin contents and essential oil composition were measured using the DPPH radical scavenging assay, Folin-Ciocalteu reagent, spectrophotometric and GC-MS methods, respectively. Growing both basil varieties under 70:30 % R:B light resulted in higher antiradical activity than that of other light spectra. Highest amount of total phenolic and anthocyanin contents were observed in leaves of plants grown under 70:30 % R:B light in green and purple varieties, respectively. GC-MS analysis showed that essential oil compounds in both basil varieties were influenced by spectral light composition. In green basil, chemical composition of essential oil was improved by growing them under 70:30 % R:B light, while in purple variety R light induced production of limonene, α-pinene and β-myrcene. Close correlations were found between some essential oil compounds in plants grown under different light spectra. The effects of light spectra on bioactive compounds were depended on variety and type of compound. Therefore, through exposure to specific light spectrum it is possible to alter bioactive compound composition in basil plants.

Ocimum basilicum L. is one of the most important medicinal and vegetable crops due to its essential oil, pleasant aroma and taste. In this study, we evaluated the impact of different light intensities, including 100 %, 50 %, and 30 % of natural sunlight, on the growth, phytochemical compositions, and antioxidant activity of green and purple basil cultivars at two different harvest times: early morning and noon. The height of the plant, number of leaves per plant, length of the petiole, diameter of the stem, and fresh and dry weight of the shoot were all reduced by decreasing light intensity in both basil cultivars. When the plants of both cultivars were grown under full light intensity and were sampled at noon, they showed the highest phenolic and flavonoid contents. The highest antioxidant activity was detected in purple basil cultivars grown under 30 and 50 % of sunlight in both harvests. The green basil cultivar showed the highest antioxidant activity when exposed to 30 % sunlight and harvested in the early morning. The highest essential oil content and yield in both basil cultivars were obtained under full sunlight in the early morning harvests. In summary, light intensity and harvest time influence the phytochemical yield, composition, and growth of two studied basil cultivars. Optimal results, particularly for medicinal purposes, were achieved by morning harvesting to maximize the essential oil yield of basils.

Basil (Ocimum basilicum L.) is one of the most important medicinal and aromatic plants. Light intensity is an indispensable factor for plants due to its effect on photosynthesis and physiological processes. Here, we investigated the impact of light intensities and harvesting times on the photosynthesis of green and purple basil. The experiment involved subjecting plants to three different levels of sunlight for 12 days: complete—100%, 50%, and 30%—sunlight. In addition, we evaluated the impact of harvest time during the day. The highest levels of photosynthetic and protective pigments were detected under full sunlight conditions in purple basil harvested at noon. The highest levels of soluble and storage carbohydrates were recorded in the purple basil grown under full sunlight and harvested during the early morning. By contrast, the lowest levels were obtained in plants grown under 30% sunlight and harvested at noon time. Under all light treatments, the maximum quantum yield of photosystem II (F_V/F_M) was detected at 4 a.m. in both basil varieties; it decreased at noon and increased again at 5 p.m. Non-Photochemical Quenching (NPQ) was most elevated in the green variety under all light intensities at noon. However, the highest NPQ was detected in the purple variety at 8 a.m. The NPQ was lowest in both basil varieties during the early morning and afternoon. Full sunlight at noon caused temporary photoinhibition and reduced carbohydrates while enhancing pigment concentration and photo-protective mechanisms in basil plants.

Gamma radiation has been suggested to have post-effects on emerging plants when applied to the seeds. In the present study, we aimed to induce alterations in photosynthetic functionality and subsequent modifications in secondary metabolites of summer savory following seed priming with gamma radiation. Savory seeds were treated with 0, 50, 100, 200, and 300 Gy gamma radiation in a completely randomized design with ten replications for morphological and photosynthetic parameters and three for phytochemical assessments. The results showed that gamma radiation on seeds adversely affected photosynthetic performance, especially at the highest doses. It negatively influenced the growth, while increasing the shoot branching, the number of nodes, and the diameter of the stem. Gamma radiation on seeds generally reduced pigmentation in savory leaves, such as chlorophylls, carotenoids, and anthocyanins. However, soluble sugar, starch, total phenolics, and total flavonoid contents were elevated in the leaves of plants that emerged from gamma-primed seeds. Gamma radiation priming reduced essential oil’s percentage and yield. Carvacrol and limonene components of essential oil were diminished, whereas linalool and thymol were increased. In conclusion, due to its inherent stress-inducing effects, and despite some positive effects on phytochemicals, seed priming with gamma radiation adversely influenced growth, photosynthesis, and quantity and quality of savory essential oils. Further research is still needed to target the use of gamma radiations before harvesting the seeds or determine the cytogenetic characteristics of irradiated plants.

Controlled environment agriculture is a promising solution to address climate change and resource limitations. Light, the primary energy source driving photosynthesis and regulating plant growth, is critical in optimizing produce quality. However, the impact of specific light spectra during night interruption on improving phytochemical content and produce quality remains underexplored. This study investigated the effects of red (peak wavelength at 660 nm) and far-red night interruption (peak wavelength at 730 nm) on photosynthetic efficiency, biomass distribution, and phytochemical production in Italian basil (Ocimum basilicum L.). Treatments included red light, far-red light, a combination of both, and a control without night interruption. Red light significantly increased chlorophyll a by 16.8%, chlorophyll b by 20.6%, and carotenoids by 11%, improving photosynthetic efficiency and nutritional quality. Red light also elevated anthocyanin levels by 15.5%, while far-red light promoted flavonoid production by 43.56%. Although red light enhanced biomass, the primary benefit was improved leaf quality, with more biomass directed to leaves over roots. Far-red light reduced transpiration, enhancing post-harvest water retention and shelf life. These findings demonstrate that red and far-red night interruption can optimize phytochemical content, produce quality, and post-harvest durability, offering valuable insights for controlled environment agriculture. Future research should focus on refining night interruption light strategies across a broader range of crops to enhance produce quality and shelf life in controlled environment agriculture.

Basil (Ocimum basilicum L.) is one of the important medicinal and aromatic plants. Light intensity is an indispensable factor for plants due to its effect on photosynthesis and physiological processes. Here, we investigated the impact of light intensities and harvesting times on the photosynthesis of green and purple basil. The experiment involved subjecting the plants to three different sunlight levels for 12 days: complete – 100%, 50%, and 30% sunlight. In addition, we scrutinize the harvesting time. The highest levels of photosynthetic and protective pigments were detected under full sunlight conditions in the purple basil harvested at noon. The highest levels of soluble and storage carbohydrates were recorded in the purple basil grown under full sunlight and harvested in the early morning. In contrast, their lowest levels were obtained in plants grown under 30% sunlight and harvested at noon time. Under all light treatments, the maximum quantum yield of photosystem II (FV/FM) was detected at 4 a.m. in both basil varieties; it decreased at noon and increased again at 5 p.m. Non-Photochemical Quenching (NPQ) got most elevated in the green variety under all light intensities at noon. However the highest NPQ was detected in the purple variety at 8 a.m. The NPQ was lowest in both basil varieties during the early morning and afternoon. Full sunlight at noon caused temporary photoinhibition and reduced car-bohydrates while enhancing pigment concentration and photo-protective mechanisms in basil plants.