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Jinsong Bao
Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Zijingang Campus Hangzhou 310058 China

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Research article
Published: 13 August 2021 in BMC Plant Biology
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Background Cadmium (Cd) is a toxic heavy metal that is harmful to the environment and human health. Cd pollution threatens the cultivation of rice (Oryza sativa L.) in many countries. Improving rice performance under Cd stress could potentially improve rice productivity. Results In this study, 9 growth traits of 188 different cultivated rice accessions under normal and Cd stress conditions were found to be highly variable during the seedling stage. Based on ~3.3 million single nucleotide polymorphisms (SNPs), 119 Cd-mediated growth response (CGR) quantitative trait loci (QTL) were identified by a genome-wide association study (GWAS), 55 of which have been validated by previously reported QTL and 64 were new CGR loci. Combined with the data from the GWAS, transcriptome analysis, gene annotations from the gene ontology (GO) Slim database, and annotations and functions of homologous genes, 148 CGR candidate genes were obtained. Additionally, several reported genes have been found to play certain roles in CGRs. Seven Cd-related cloned genes were found among the CGR genes. Natural elite haplotypes/alleles in these genes that increased Cd tolerance were identified by a haplotype analysis of a diverse mini core collection. More importantly, this study was the first to uncover the natural variations of 5 GST genes that play important roles in CGRs. Conclusion The exploration of Cd-resistant rice germplasm resources and the identification of elite natural variations related to Cd-resistance will help improve the tolerance of current major rice varieties to Cd, as well as provide raw materials and new genes for breeding Cd-resistant varieties.

ACS Style

Jianping Yu; Chaolei Liu; Hai Lin; Bin Zhang; Xiaoxia Li; Qiaoling Yuan; Tianjiao Liu; Huiying He; Zhaoran Wei; Shilin Ding; Chao Zhang; Hongsheng Gao; Longbiao Guo; Quan Wang; Qian Qian; Lianguang Shang. Loci and natural alleles for cadmium-mediated growth responses revealed by a genome wide association study and transcriptome analysis in rice. BMC Plant Biology 2021, 21, 1 -15.

AMA Style

Jianping Yu, Chaolei Liu, Hai Lin, Bin Zhang, Xiaoxia Li, Qiaoling Yuan, Tianjiao Liu, Huiying He, Zhaoran Wei, Shilin Ding, Chao Zhang, Hongsheng Gao, Longbiao Guo, Quan Wang, Qian Qian, Lianguang Shang. Loci and natural alleles for cadmium-mediated growth responses revealed by a genome wide association study and transcriptome analysis in rice. BMC Plant Biology. 2021; 21 (1):1-15.

Chicago/Turabian Style

Jianping Yu; Chaolei Liu; Hai Lin; Bin Zhang; Xiaoxia Li; Qiaoling Yuan; Tianjiao Liu; Huiying He; Zhaoran Wei; Shilin Ding; Chao Zhang; Hongsheng Gao; Longbiao Guo; Quan Wang; Qian Qian; Lianguang Shang. 2021. "Loci and natural alleles for cadmium-mediated growth responses revealed by a genome wide association study and transcriptome analysis in rice." BMC Plant Biology 21, no. 1: 1-15.

Full paper
Published: 14 July 2021 in New Phytologist
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Heat stress is a major environmental threat affecting crop growth and productivity. However, the molecular mechanisms associated with plant responses to heat stress are poorly understood. Here, we identified a heat stress-sensitive mutant, hts1, in rice. HTS1 encodes a thylakoid membrane-localized β-ketoacyl carrier protein reductase (KAR) involved in de novo fatty acid biosynthesis. Phylogenetic and bioinformatic analysis showed that HTS1 probably originated from streptophyte algae and is evolutionarily conserved in land plants. Thermostable HTS1 is predominantly expressed in green tissues and strongly induced by heat stress, but is less responsive to salinity, cold and drought treatments. An amino acid substitution at A254T in HTS1 causes a significant decrease in KAR enzymatic activity and, consequently, impairs fatty acid synthesis and lipid metabolism in the hts1 mutant, especially under heat stress. Compared to the wild-type, the hts1 mutant exhibited heat-induced higher H2O2 accumulation, a larger Ca2+ influx to mesophyll cells, and more damage to membranes and chloroplasts. Also, disrupted heat stress signaling in the hts1 mutant depresses the transcriptional activation of HsfA2s and the downstream target genes. We suggest that HTS1 is critical for underpinning membrane stability, chloroplast integrity and stress signaling for heat tolerance in rice.

ACS Style

Fei Chen; Guojun Dong; Fang Wang; Yingqi Shi; Jiayu Zhu; Yanli Zhang; Banpu Ruan; Yepin Wu; Xue Feng; Chenchen Zhao; Miing T. Yong; Paul Holford; Dali Zeng; Qian Qian; Limin Wu; Zhong‐Hua Chen; Yanchun Yu. A β‐Ketoacyl carrier protein reductase confers heat tolerance via the regulation of fatty acid biosynthesis and stress signaling in rice. New Phytologist 2021, 1 .

AMA Style

Fei Chen, Guojun Dong, Fang Wang, Yingqi Shi, Jiayu Zhu, Yanli Zhang, Banpu Ruan, Yepin Wu, Xue Feng, Chenchen Zhao, Miing T. Yong, Paul Holford, Dali Zeng, Qian Qian, Limin Wu, Zhong‐Hua Chen, Yanchun Yu. A β‐Ketoacyl carrier protein reductase confers heat tolerance via the regulation of fatty acid biosynthesis and stress signaling in rice. New Phytologist. 2021; ():1.

Chicago/Turabian Style

Fei Chen; Guojun Dong; Fang Wang; Yingqi Shi; Jiayu Zhu; Yanli Zhang; Banpu Ruan; Yepin Wu; Xue Feng; Chenchen Zhao; Miing T. Yong; Paul Holford; Dali Zeng; Qian Qian; Limin Wu; Zhong‐Hua Chen; Yanchun Yu. 2021. "A β‐Ketoacyl carrier protein reductase confers heat tolerance via the regulation of fatty acid biosynthesis and stress signaling in rice." New Phytologist , no. : 1.

Review
Published: 29 June 2021 in Agriculture
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Rice (Oryza sativa L.) is one of the most important cereal crops in the world. The identification of sd1 mutants in rice resulted in a semi-dwarf phenotype that was used by breeders to improve yields. Investigations of sd1 mutants initiated the “green revolution” for rice and staved off famine for many people in the 1960s. The smaller plant height conferred by sd1 allele gives the plants lodging resistance even with a high amount of nitrogen fertilizer. Guang-chang-ai-carrying sd1 was the first high-yielding rice variety that capitalized on the semi-dwarf trait, aiming to significantly improve the rice yield in China. IR8, known as the miracle rice, was also bred by using sd1. The green revolution gene sd1 in rice has been used for decades, but was not identified for a long time. The SD1 gene encodes the rice Gibberellin 20 oxidase-2 (GA20ox2). As such, the SD1 gene is instrumental in uncovering the molecular mechanisms underlying gibberellin biosynthesis There are ten different alleles of SD1. These alleles are identified by genome sequencing within several donor lines in breeding for semi-dwarf rice. Apart from breeding applications and the molecular mechanism of GA biosynthesis, the SD1 gene is also involved in the molecular regulation of other important agronomic traits, like nitrogen fertilizer utilization. The dentification of new alleles of SD1 can be obtained by mutagenesis and genome editing. These new alleles will play an important role in improving the resource diversity of semi-dwarf breeding in the future.

ACS Style

YouLin Peng; Yungao Hu; Qian Qian; Deyong Ren. Progress and Prospect of Breeding Utilization of Green Revolution Gene SD1 in Rice. Agriculture 2021, 11, 611 .

AMA Style

YouLin Peng, Yungao Hu, Qian Qian, Deyong Ren. Progress and Prospect of Breeding Utilization of Green Revolution Gene SD1 in Rice. Agriculture. 2021; 11 (7):611.

Chicago/Turabian Style

YouLin Peng; Yungao Hu; Qian Qian; Deyong Ren. 2021. "Progress and Prospect of Breeding Utilization of Green Revolution Gene SD1 in Rice." Agriculture 11, no. 7: 611.

Journal article
Published: 29 June 2021 in Agronomy
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Salinity is a major abiotic stressor that leads to productivity losses in rice (Oryza sativa L.). In this study, transcriptome profiling and heterosis-related genes were analyzed by ribonucleic acid sequencing (RNA-Seq) in seedlings of a mega rice hybrid, Liang-You-Pei-Jiu (LYP9), and its two parents 93–11 and Pei-ai64s (PA64s), under control and two different salinity levels, where we found 8292, 8037, and 631 salt-induced differentially expressed genes (DEGs), respectively. Heterosis-related DEGs were obtained higher after 14 days of salt treatment than after 7 days. There were 631 and 4237 salt-induced DEGs related to heterosis under 7-day and 14-day salt stresses, respectively. Gene functional classification showed the expression of genes involved in photosynthesis activity after 7-day stress treatment, and in metabolic and catabolic activity after 14 days. In addition, we correlated the concurrence of an expression of DEGs for the bHLH transcription factor and a shoot length/salinity-related quantitative trait locus qSL7 that we fine-mapped previously, providing a confirmed case of heterosis-related genes. This experiment reveals the transcriptomic divergence of the rice F1 hybrid and its parental lines under control and salt stress state, and enlightens about the significant molecular mechanisms developed over time in response to salt stress.

ACS Style

Noushin Jahan; Yang Lv; Mengqiu Song; Yu Zhang; Lianguang Shang; Ying Lu; Guoyou Ye; Qian Qian; Zhenyu Gao; Longbiao Guo. Transcriptomic Analysis of Short-Term Salt-Stress Response in Mega Hybrid Rice Seedlings. Agronomy 2021, 11, 1328 .

AMA Style

Noushin Jahan, Yang Lv, Mengqiu Song, Yu Zhang, Lianguang Shang, Ying Lu, Guoyou Ye, Qian Qian, Zhenyu Gao, Longbiao Guo. Transcriptomic Analysis of Short-Term Salt-Stress Response in Mega Hybrid Rice Seedlings. Agronomy. 2021; 11 (7):1328.

Chicago/Turabian Style

Noushin Jahan; Yang Lv; Mengqiu Song; Yu Zhang; Lianguang Shang; Ying Lu; Guoyou Ye; Qian Qian; Zhenyu Gao; Longbiao Guo. 2021. "Transcriptomic Analysis of Short-Term Salt-Stress Response in Mega Hybrid Rice Seedlings." Agronomy 11, no. 7: 1328.

Research article
Published: 26 June 2021 in Cereal Chemistry
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Background and objectives The domestication of cultivated rice (Oryza sativa L.) has long been studied, and many agronomically relevant genes have undergone artificial selection. Among them, the selection history of the starch synthase IIa (SSIIa) has not been fully discovered. The SSIIa is a genetic factor that controls the gelatinization temperature (GT) of rice starch, an important parameter for rice cooking quality. Findings The A/GC haplotype in SSIIa only presented in the japonica subspecies. GT was found to be strongly associated with SNPs in SSIIa, that is, G/GC haplotype rice had high or intermediate GT, while G/TT or A/GC haplotype rice had low GT. The nucleotide diversity (π) in japonica was much lower than that of indica and wild rice, indicating that a population bottleneck was experienced during japonica rice domestication. A possible positive selection on the A SNP of SSIIa in temperate japonica rice favoring low GT starch synthesis was proposed. Conclusions The evolution of SSIIa in the japonica rice was possibly under positive selection favoring low GT rice, which requires low energy input for cooking. The A SNP of SSIIa is only present in the japonica rice, which might be derived from a recent mutation. There are two ways, A/GC and G/TT, to make japonica rice with low GT, explaining why all the temperate japonica rice cultivars have low GT. Significance and novelty Findings of this study provide an understanding of why all the temperate japonica rice have low GT starch, because there are two ways to make it GT low.

ACS Style

Jiling Song; Yaqi Hu; Guofu Deng; Gaoxing Dai; Jinsong Bao. The origin of the A/G SNP of SSIIa in rice and its relation to gelatinization temperature. Cereal Chemistry 2021, 1 .

AMA Style

Jiling Song, Yaqi Hu, Guofu Deng, Gaoxing Dai, Jinsong Bao. The origin of the A/G SNP of SSIIa in rice and its relation to gelatinization temperature. Cereal Chemistry. 2021; ():1.

Chicago/Turabian Style

Jiling Song; Yaqi Hu; Guofu Deng; Gaoxing Dai; Jinsong Bao. 2021. "The origin of the A/G SNP of SSIIa in rice and its relation to gelatinization temperature." Cereal Chemistry , no. : 1.

Research article
Published: 26 June 2021 in Molecular Plant
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Grain size is one of the most import factors of controlling rice yield, as it is associated with grain weight (GW). To date, several rice genes that regulate grain size have been isolated; however, the regulatory mechanism underlying GW control is not fully understood. Herein, a quantitative trait locus qGL5 for grain length (GL) and GW was identified in recombinant inbred lines of 9311 and Nipponbare (NPB), and fine mapped to a candidate gene, OsAUX3. Sequence variations between 9311 and NPB in the OsAUX3 promoter, and loss-of-function of OsAUX3 led to increased GL and GW. RNA-sequencing, gene expression quantification, dual-luciferase reporter assay, chromatin immunoprecipitation-quantitative polymerase chain reaction, and yeast one-hybrid assay demonstrated that OsARF6 is an upstream transcription factor of OsAUX3. OsARF6 directly binds to the auxin response elements of the OsAUX3 promoter, covering a single nucleotide polymorphism site between 9311 and NPB/Dongjin/Hwayoung, thereby controlling GL by altering longitudinal expansion and auxin distribution/content in glume cells. miR167a was also confirmed to positively regulate GL and GW by directing OsARF6 mRNA silencing. Therefore, the miR167a-OsARF6-OsAUX3 module regulates GL and GW in rice, representing a potential target for improving rice yield.

ACS Style

Jiyue Qiao; Hongzhen Jiang; Yuqing Lin; Lianguang Shang; Mei Wang; Dongming Li; Xiangdong Fu; Markus Geisler; Yanhua Qi; Zhenyu Gao; Qian Qian. A novel miR167a-OsARF6-OsAUX3 module regulates grain length and weight in rice. Molecular Plant 2021, 1 .

AMA Style

Jiyue Qiao, Hongzhen Jiang, Yuqing Lin, Lianguang Shang, Mei Wang, Dongming Li, Xiangdong Fu, Markus Geisler, Yanhua Qi, Zhenyu Gao, Qian Qian. A novel miR167a-OsARF6-OsAUX3 module regulates grain length and weight in rice. Molecular Plant. 2021; ():1.

Chicago/Turabian Style

Jiyue Qiao; Hongzhen Jiang; Yuqing Lin; Lianguang Shang; Mei Wang; Dongming Li; Xiangdong Fu; Markus Geisler; Yanhua Qi; Zhenyu Gao; Qian Qian. 2021. "A novel miR167a-OsARF6-OsAUX3 module regulates grain length and weight in rice." Molecular Plant , no. : 1.

Research article
Published: 10 June 2021 in Journal of Integrative Plant Biology
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Among the five members of AUX1/LAX genes coding for auxin carriers in rice, only OsAUX1 and OsAUX3 have been reported. To understand the function of the other AUX1/LAX genes, two independent alleles of osaux4 mutants, osaux4-1, and osaux4-2, were constructed using the CRISPR/Cas9 editing system. Homozygous osaux4-1 or osaux4-2 exhibited shorter primary root (PR) and longer root hair (RH) compared to the wild-type Dongjin (WT/DJ), and lost response to IAA treatment. OsAUX4 is intensively expressed in roots and localized on the plasma membrane, suggesting that OsAUX4 might function in the regulation of root development. The decreased meristem cell division activity and the downregulated expression of cell cycle genes in root apices of osaux4 mutants supported the hypothesis that OsAUX4 positively regulates PR elongation. OsAUX4 is expressed in RH, and osaux4 mutants showing longer RH compared to WT/DJ implies that OsAUX4 negatively regulates RH development. Furthermore, osaux4 mutants are insensitive to phosphate starvation (-Pi) and OsAUX4 effects on the -Pi response is associated with altered expression levels of Pi starvation regulated genes, and auxin distribution/contents. This study revealed that OsAUX4 not only regulates PR and RH development but also plays a regulatory role in crosstalk between auxin and -Pi signaling.

ACS Style

Rigui Ye; Yunrong Wu; Zhenyu Gao; Hao Chen; Lixia Jia; Dongming Li; Xugang Li; Qian Qian; Yanhua Qi. Primary root and root hair development regulation by OsAUX4 and its participation in the phosphate starvation response. Journal of Integrative Plant Biology 2021, 63, 1555 -1567.

AMA Style

Rigui Ye, Yunrong Wu, Zhenyu Gao, Hao Chen, Lixia Jia, Dongming Li, Xugang Li, Qian Qian, Yanhua Qi. Primary root and root hair development regulation by OsAUX4 and its participation in the phosphate starvation response. Journal of Integrative Plant Biology. 2021; 63 (8):1555-1567.

Chicago/Turabian Style

Rigui Ye; Yunrong Wu; Zhenyu Gao; Hao Chen; Lixia Jia; Dongming Li; Xugang Li; Qian Qian; Yanhua Qi. 2021. "Primary root and root hair development regulation by OsAUX4 and its participation in the phosphate starvation response." Journal of Integrative Plant Biology 63, no. 8: 1555-1567.

Review
Published: 31 May 2021 in International Journal of Molecular Sciences
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Rice (Oryza sativa L.) is a foremost staple food for approximately half the world’s population. The components of rice starch, amylose, and amylopectin are synthesized by a series of enzymes, which are responsible for rice starch properties and functionality, and then affect rice cooking and eating quality. Recently, proteomics technology has been applied to the establishment of the differentially expressed starch biosynthesis-related proteins and the identification of posttranslational modifications (PTMs) target starch biosynthesis proteins as well. It is necessary to summarize the recent studies in proteomics and PTMs in rice endosperm to deepen our understanding of starch biosynthesis protein expression and regulation, which will provide useful information to rice breeding programs and industrial starch applications. The review provides a comprehensive summary of proteins and PTMs involved in starch biosynthesis based on proteomic studies of rice developing seeds. Starch biosynthesis proteins in rice seeds were differentially expressed in the developing seeds at different developmental stages. All the proteins involving in starch biosynthesis were identified using proteomics methods. Most starch biosynthesis-related proteins are basically increased at 6–20 days after flowering (DAF) and decreased upon the high-temperature conditions. A total of 10, 14, 2, 17, and 7 starch biosynthesis related proteins were identified to be targeted by phosphorylation, lysine acetylation, succinylation, lysine 2-hydroxyisobutyrylation, and malonylation, respectively. The phosphoglucomutase is commonly targeted by five PTMs types. Research on the function of phosphorylation in multiple enzyme complex formation in endosperm starch biosynthesis is underway, while the functions of other PTMs in starch biosynthesis are necessary to be conducted in the near future.

ACS Style

Piengtawan Tappiban; Yining Ying; Feifei Xu; Jinsong Bao. Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice. International Journal of Molecular Sciences 2021, 22, 5901 .

AMA Style

Piengtawan Tappiban, Yining Ying, Feifei Xu, Jinsong Bao. Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice. International Journal of Molecular Sciences. 2021; 22 (11):5901.

Chicago/Turabian Style

Piengtawan Tappiban; Yining Ying; Feifei Xu; Jinsong Bao. 2021. "Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice." International Journal of Molecular Sciences 22, no. 11: 5901.

Brief communication
Published: 28 May 2021 in Plant Biotechnology Journal
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Since the "first green revolution" in the 1960s, rice grain yield has risen sharply. However, due to the continual decreasing of cropping areas, further increase in yield potentials is urgently demanding. Long-term excessive fertilization has led to uncontrolled retention of nitrogen fertilizer in the soil and serious pollution of the environment.

ACS Style

Jing Xu; Lianguang Shang; Jiajia Wang; Minmin Chen; Xue Fu; Huiying He; Zian Wang; Dali Zeng; Li Zhu; Jiang Hu; Chao Zhang; Guang Chen; Zhenyu Gao; Weiwei Zou; Deyong Ren; Guojun Dong; Lan Shen; Qiang Zhang; Qing Li; Longbiao Guo; Qian Qian; Guangheng Zhang. The SEEDLING BIOMASS 1 allele from indica rice enhances yield performance under low‐nitrogen environments. Plant Biotechnology Journal 2021, 1 .

AMA Style

Jing Xu, Lianguang Shang, Jiajia Wang, Minmin Chen, Xue Fu, Huiying He, Zian Wang, Dali Zeng, Li Zhu, Jiang Hu, Chao Zhang, Guang Chen, Zhenyu Gao, Weiwei Zou, Deyong Ren, Guojun Dong, Lan Shen, Qiang Zhang, Qing Li, Longbiao Guo, Qian Qian, Guangheng Zhang. The SEEDLING BIOMASS 1 allele from indica rice enhances yield performance under low‐nitrogen environments. Plant Biotechnology Journal. 2021; ():1.

Chicago/Turabian Style

Jing Xu; Lianguang Shang; Jiajia Wang; Minmin Chen; Xue Fu; Huiying He; Zian Wang; Dali Zeng; Li Zhu; Jiang Hu; Chao Zhang; Guang Chen; Zhenyu Gao; Weiwei Zou; Deyong Ren; Guojun Dong; Lan Shen; Qiang Zhang; Qing Li; Longbiao Guo; Qian Qian; Guangheng Zhang. 2021. "The SEEDLING BIOMASS 1 allele from indica rice enhances yield performance under low‐nitrogen environments." Plant Biotechnology Journal , no. : 1.

Short communication
Published: 14 May 2021 in Plant Signaling & Behavior
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Ferredoxins (Fds) play a unique and important role in photosynthetic electron transport. Recently, we characterized the function of Fd1 in rice (Oryza sativa L.), showing that Fd1 is the primary photosynthetic electron transport protein and that Fd1 participates in carbon assimilation. However, the subcellular localization and specific functions of other Fds in rice are not yet fully understood. Here, our subcellular localization analysis of the seven Fds in rice showed that they are located in the chloroplasts of photosynthetic tissues and the plastids of non-photosynthetic tissues. Moreover, qRT-PCR indicated that Fd1 transcript levels were highest in photosynthetic tissues, while Fd4 transcript levels were highest in non-photosynthetic tissues. Collectively, our results suggest that rice Fds are located in chloroplasts/plastids, but may function in different tissues, and Fd4 may be a non-photosynthetic type Fd.

ACS Style

Lei He; Man Li; Dongdong Chen; Qian Qian; Dali Zeng; Li Zhu. Rice Ferredoxins localize to chloroplasts/plastids and may function in different tissues. Plant Signaling & Behavior 2021, 1926813 .

AMA Style

Lei He, Man Li, Dongdong Chen, Qian Qian, Dali Zeng, Li Zhu. Rice Ferredoxins localize to chloroplasts/plastids and may function in different tissues. Plant Signaling & Behavior. 2021; ():1926813.

Chicago/Turabian Style

Lei He; Man Li; Dongdong Chen; Qian Qian; Dali Zeng; Li Zhu. 2021. "Rice Ferredoxins localize to chloroplasts/plastids and may function in different tissues." Plant Signaling & Behavior , no. : 1926813.

Preprint content
Published: 14 May 2021
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Sika deer are known to prefer oak leaves, which are rich in tannins and toxic to most mammals; however, the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear. In identifying the mechanism responsible for the tolerance of a highly toxic diet, we have made a major advancement in the elucidation of the genomics of sika deer. We generated the first high-quality, chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments. Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food, especially the expansion of UGT genes in a subfamily. The first chromosome-level assembly and genetic characterization of the tolerance toa highly toxic diet suggest that the sika deer genome will serve as an essential resource for understanding evolutionary events and tannin adaptation. Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.

ACS Style

Xiumei Xing; Cheng Ai; Tianjiao Wang; Yang Li; Huitao Liu; Pengfei Hu; Guiwu Wang; Huamiao Liu; Hongliang Wang; Ranran Zhang; Junjun Zheng; Xiaobo Wang; Lei Wang; Yuxiao Chang; Qian Qian; Jinghua Yu; Lixin Tang; Shigang Wu; Xiujuan Shao; Alun Li; Peng Cui; Wei Zhan; Sheng Zhao; Zhichao Wu; Xiqun Shao; Yimeng Dong; Min Rong; Yihong Tan; Xuezhe Cui; Shuzhuo Chang; Xingchao Song; Tongao Yang; Limin Sun; Yan Ju; Pei Zhao; Huanhuan Fan; Ying Liu; Xinhui Wang; Wanyun Yang; Min Yang; Tao Wei; Shanshan Song; Jiaping Xu; Zhigang Yue; QiQi Liang; Chunyi Li; Jue Ruan; Fuhe Yang. The First High-Quality Reference Genome of Sika Deer Provides Insights for High-Tannin Adaptation. 2021, 1 .

AMA Style

Xiumei Xing, Cheng Ai, Tianjiao Wang, Yang Li, Huitao Liu, Pengfei Hu, Guiwu Wang, Huamiao Liu, Hongliang Wang, Ranran Zhang, Junjun Zheng, Xiaobo Wang, Lei Wang, Yuxiao Chang, Qian Qian, Jinghua Yu, Lixin Tang, Shigang Wu, Xiujuan Shao, Alun Li, Peng Cui, Wei Zhan, Sheng Zhao, Zhichao Wu, Xiqun Shao, Yimeng Dong, Min Rong, Yihong Tan, Xuezhe Cui, Shuzhuo Chang, Xingchao Song, Tongao Yang, Limin Sun, Yan Ju, Pei Zhao, Huanhuan Fan, Ying Liu, Xinhui Wang, Wanyun Yang, Min Yang, Tao Wei, Shanshan Song, Jiaping Xu, Zhigang Yue, QiQi Liang, Chunyi Li, Jue Ruan, Fuhe Yang. The First High-Quality Reference Genome of Sika Deer Provides Insights for High-Tannin Adaptation. . 2021; ():1.

Chicago/Turabian Style

Xiumei Xing; Cheng Ai; Tianjiao Wang; Yang Li; Huitao Liu; Pengfei Hu; Guiwu Wang; Huamiao Liu; Hongliang Wang; Ranran Zhang; Junjun Zheng; Xiaobo Wang; Lei Wang; Yuxiao Chang; Qian Qian; Jinghua Yu; Lixin Tang; Shigang Wu; Xiujuan Shao; Alun Li; Peng Cui; Wei Zhan; Sheng Zhao; Zhichao Wu; Xiqun Shao; Yimeng Dong; Min Rong; Yihong Tan; Xuezhe Cui; Shuzhuo Chang; Xingchao Song; Tongao Yang; Limin Sun; Yan Ju; Pei Zhao; Huanhuan Fan; Ying Liu; Xinhui Wang; Wanyun Yang; Min Yang; Tao Wei; Shanshan Song; Jiaping Xu; Zhigang Yue; QiQi Liang; Chunyi Li; Jue Ruan; Fuhe Yang. 2021. "The First High-Quality Reference Genome of Sika Deer Provides Insights for High-Tannin Adaptation." , no. : 1.

Journal article
Published: 27 April 2021 in Molecular Plant
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Regulation of seed size is a key strategy for improving crop yield, and is also a basic biological question, but how plants determine their seed size remains elusive. Here we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice. WG1, which encodes a glutaredoxin protein, promotes grain growth by increasing cell proliferation. WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1, indicating that WG1 may act as adaptor protein to recruit the transcriptional co-repressor. OsbZIP47 restricts grain growth by decreasing cell proliferation. Further results reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation. Genetic analyses support that GW2, WG1 and OsbZIP47 function in a common pathway to control grain growth. Thus, our findings reveal a genetic and molecular framework for the GW2-WG1-OsbZIP47 regulatory module-mediated control of grain size and weight, opening new perspectives for using this regulatory pathway for improvement of seed size and weight in crops.

ACS Style

Jianqin Hao; Dekai Wang; Yingbao Wu; Ke Huang; Penggen Duan; Na Li; Ran Xu; Dali Zeng; Guojun Dong; Baolan Zhang; Limin Zhang; Dirk Inzé; Qian Qian; Yunhai Li. The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice. Molecular Plant 2021, 1 .

AMA Style

Jianqin Hao, Dekai Wang, Yingbao Wu, Ke Huang, Penggen Duan, Na Li, Ran Xu, Dali Zeng, Guojun Dong, Baolan Zhang, Limin Zhang, Dirk Inzé, Qian Qian, Yunhai Li. The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice. Molecular Plant. 2021; ():1.

Chicago/Turabian Style

Jianqin Hao; Dekai Wang; Yingbao Wu; Ke Huang; Penggen Duan; Na Li; Ran Xu; Dali Zeng; Guojun Dong; Baolan Zhang; Limin Zhang; Dirk Inzé; Qian Qian; Yunhai Li. 2021. "The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice." Molecular Plant , no. : 1.

Journal article
Published: 07 April 2021 in Plant Science
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Chloroplasts are closely associated with the growth and development of higher plants. Accumulating evidence has revealed that the multiple organellar RNA editing factors (MORF) family of proteins influences plastidic and mitochondrial development through post-transcriptional regulation. However, the role of MORFs in regulating the development of chloroplasts in rice is still unclear. The OsMORF9 gene belongs to a small family of 7 genes in rice and is highly expressed in young leaves. We used the CRISPR/Cas9 system to mutate OsMORF9. The resulting knockout lines osmorf9−1 and osmorf9−2 exhibited an albino seedling lethal phenotype. Besides, the expression of many plastid-encoded genes involved in photosynthesis, the biogenesis of plastidic ribosomes and the editing and splicing of specific plastidic RNA molecules were severely affected in these two OsMORF9 mutants. Furthermore, yeast two-hybrid analysis revealed that OsMORF9 could interact with OsSLA4 and DUA1 which are members of the pentatricopeptide repeat (PPR) family of proteins. Analysis of subcellular localization of OsMORF9 also suggested that it might function in chloroplasts. The findings from the present study demonstrated the critical role of OsMORF9 in the biogenesis of chloroplast ribosomes, chloroplast development and seedling survival. This therefore provides new insights on the function of MORF proteins in rice.

ACS Style

Qiang Zhang; Yaliang Wang; Wei Xie; Changzhao Chen; Deyong Ren; Jiang Hu; Li Zhu; Guangheng Zhang; Zhenyu Gao; Longbiao Guo; Dali Zeng; Lan Shen; Qian Qian. OsMORF9 is necessary for chloroplast development and seedling survival in rice. Plant Science 2021, 307, 110907 .

AMA Style

Qiang Zhang, Yaliang Wang, Wei Xie, Changzhao Chen, Deyong Ren, Jiang Hu, Li Zhu, Guangheng Zhang, Zhenyu Gao, Longbiao Guo, Dali Zeng, Lan Shen, Qian Qian. OsMORF9 is necessary for chloroplast development and seedling survival in rice. Plant Science. 2021; 307 ():110907.

Chicago/Turabian Style

Qiang Zhang; Yaliang Wang; Wei Xie; Changzhao Chen; Deyong Ren; Jiang Hu; Li Zhu; Guangheng Zhang; Zhenyu Gao; Longbiao Guo; Dali Zeng; Lan Shen; Qian Qian. 2021. "OsMORF9 is necessary for chloroplast development and seedling survival in rice." Plant Science 307, no. : 110907.

Journal article
Published: 24 February 2021 in Food Hydrocolloids
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The fine structure-function (physicochemical properties) relationships of tea seed starches from seven cultivars were investigated. Tea seed starch granules were small size (2.4–11.8 μm) and round with an A-type X-ray diffraction pattern. The amylose content (AC) ranged from 29.8 to 33.5%, and was positively correlated with setback viscosity (SB), but not correlated with the gel hardness (HD). The peak gelatinization temperature (Tp) was low (67 °C) for one cultivar while intermediate (71–74 °C) for the others. AC was also negatively correlated with the relative crystallinity (RC) of tea starches. The amount of long B chains and average chain length of amylopectin were positively correlated with RC, which determined the Tp. The relative length of amylopectin peak 2 (XAP2) was negatively correlated with SB. The amount of amylopectin A chains showed a negative correlation with HD and hot paste viscosity (HPV). The current findings will be helpful to increase the understanding of tea seed starch quality for its application in food processing.

ACS Style

Lisheng Qian; Yao Yao; Cheng Li; Feifei Xu; Yinin Ying; Zongqing Shao; Jinsong Bao. Pasting, gelatinization, and retrogradation characteristics related to structural properties of tea seed starches. Food Hydrocolloids 2021, 117, 106701 .

AMA Style

Lisheng Qian, Yao Yao, Cheng Li, Feifei Xu, Yinin Ying, Zongqing Shao, Jinsong Bao. Pasting, gelatinization, and retrogradation characteristics related to structural properties of tea seed starches. Food Hydrocolloids. 2021; 117 ():106701.

Chicago/Turabian Style

Lisheng Qian; Yao Yao; Cheng Li; Feifei Xu; Yinin Ying; Zongqing Shao; Jinsong Bao. 2021. "Pasting, gelatinization, and retrogradation characteristics related to structural properties of tea seed starches." Food Hydrocolloids 117, no. : 106701.

Research article
Published: 09 February 2021 in BMC Plant Biology
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Background Rice plants suffer from the rising temperature which is becoming more and more prominent. Mining heat-resistant genes and applying them to rice breeding is a feasible and effective way to solve the problem. Result Three main biomass traits, including shoot length, dry weight, and fresh weight, changed after abnormally high-temperature treatment in the rice seedling stage of a recombinant inbred lines and the natural indica germplasm population. Based on a comparison of the results of linkage analysis and genome-wide association analysis, two loci with lengths of 57 kb and 69 kb in qDW7 and qFW6, respectively, were associated with the rice response to abnormally high temperatures at the seedling stage. Meanwhile, based on integrated transcriptome analysis, some genes are considered as important candidate genes. Combining with known genes and analysis of homologous genes, it was found that there are eight genes in candidate intervals that need to be focused on in subsequent research. Conclusions The results indicated several relevant loci, which would help researchers to further discover beneficial heat-resistant genes that can be applied to rice heat-resistant breeding.

ACS Style

Zhaoran Wei; Qiaoling Yuan; Hai Lin; Xiaoxia Li; Chao Zhang; Hongsheng Gao; Bin Zhang; Huiying He; Tianjiao Liu; Zhang Jie; Xu Gao; Shandang Shi; Bo Wang; Zhenyu Gao; Lingrang Kong; Qian Qian; Lianguang Shang. Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress. BMC Plant Biology 2021, 21, 1 -13.

AMA Style

Zhaoran Wei, Qiaoling Yuan, Hai Lin, Xiaoxia Li, Chao Zhang, Hongsheng Gao, Bin Zhang, Huiying He, Tianjiao Liu, Zhang Jie, Xu Gao, Shandang Shi, Bo Wang, Zhenyu Gao, Lingrang Kong, Qian Qian, Lianguang Shang. Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress. BMC Plant Biology. 2021; 21 (1):1-13.

Chicago/Turabian Style

Zhaoran Wei; Qiaoling Yuan; Hai Lin; Xiaoxia Li; Chao Zhang; Hongsheng Gao; Bin Zhang; Huiying He; Tianjiao Liu; Zhang Jie; Xu Gao; Shandang Shi; Bo Wang; Zhenyu Gao; Lingrang Kong; Qian Qian; Lianguang Shang. 2021. "Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress." BMC Plant Biology 21, no. 1: 1-13.

Accepted manuscript
Published: 02 February 2021 in The Plant Cell
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Panicle size and grain number are important agronomic traits and influence grain yield in rice (Oryza sativa), but the molecular and genetic mechanisms underlying panicle size and grain number control remain largely unknown in crops. Here we report that LARGE2 encodes a HECT-domain E3 ubiquitin ligase OsUPL2 and regulates panicle size and grain number in rice. The loss of function large2 mutants produce large panicles with increased grain number, wide grains and leaves, and thick culms. LARGE2 regulates panicle size and grain number by repressing meristematic activity. LARGE2 is highly expressed in young panicles and grains. Biochemical analyses show that LARGE2 physically associates with ABERRANT PANICLE ORGANIZATION1 (APO1) and APO2, two positive regulators of panicle size and grain number, and modulates their stabilities. Genetic analyses support that LARGE2 functions with APO1 and APO2 in a common pathway to regulate panicle size and grain number. These findings reveal a novel genetic and molecular mechanism of the LARGE2-APO1/APO2 module-mediated control of panicle size and grain number in rice, suggesting that this module is a promising target for improving panicle size and grain number in crops.

ACS Style

Luojiang Huang; Kai Hua; Ran Xu; Dali Zeng; Ruci Wang; Guojun Dong; Guozheng Zhang; Xueli Lu; Na Fang; Dekai Wang; Penggen Duan; Baolan Zhang; Zupei Liu; Na Li; Yuehua Luo; Qian Qian; Shanguo Yao; Yunhai Li. The LARGE2-APO1/APO2 regulatory module controls panicle size and grain number in rice. The Plant Cell 2021, 33, 1212 -1228.

AMA Style

Luojiang Huang, Kai Hua, Ran Xu, Dali Zeng, Ruci Wang, Guojun Dong, Guozheng Zhang, Xueli Lu, Na Fang, Dekai Wang, Penggen Duan, Baolan Zhang, Zupei Liu, Na Li, Yuehua Luo, Qian Qian, Shanguo Yao, Yunhai Li. The LARGE2-APO1/APO2 regulatory module controls panicle size and grain number in rice. The Plant Cell. 2021; 33 (4):1212-1228.

Chicago/Turabian Style

Luojiang Huang; Kai Hua; Ran Xu; Dali Zeng; Ruci Wang; Guojun Dong; Guozheng Zhang; Xueli Lu; Na Fang; Dekai Wang; Penggen Duan; Baolan Zhang; Zupei Liu; Na Li; Yuehua Luo; Qian Qian; Shanguo Yao; Yunhai Li. 2021. "The LARGE2-APO1/APO2 regulatory module controls panicle size and grain number in rice." The Plant Cell 33, no. 4: 1212-1228.

Research article
Published: 13 January 2021 in Plant Biotechnology Journal
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CRISPR‐Cas9 is an emerging genome editing tool for reverse genetics in plants. However, its application for functional study of non‐coding RNAs in plants is still at its infancy. Despite being a major class of non‐coding RNAs, the biological roles of circle RNAs (circRNAs) remains largely unknown in plants. Previous plant circRNA studies have focused on identification and annotation of putative circRNAs, with their functions largely uninvestigated by genetic approaches. Here, we applied a multiplexed CRISPR‐Cas9 strategy to efficiently acquire individual null mutants for four circRNAs in rice. We showed each of these rice circRNA loci (Os02circ25329, Os06circ02797, Os03circ00204 and Os05circ02465) can be deleted at 10% or higher efficiency in both protoplasts and stable transgenic T0 lines. Such high efficiency deletion enabled the generation of circRNA null allele plants without the CRISPR‐Cas9 transgene in the T1 generation. Characterization of the mutants reveals these circRNAs’ participation in salt stress response during seed germination and in particular the Os05circ02465 null mutant showed high salt tolerance. Notably, the seedlings of the Os06circ02797 mutant showed rapid growth phenotype after seed germination with the seedlings containing higher chlorophyll A/B content. Further molecular and computational analyses suggested a circRNA‐miRNA‐mRNA regulatory network where Os06circ02797 functions to bind and sequester OsMIR408, an important and conserved microRNA in plants. This study not only presents genetic evidence for the first time in plants that certain circRNAs may serve as sponges to negatively regulate miRNAs, a phenomenon previously demonstrated in mammalian cells, but also provides important insights for improving agronomic traits through gene editing of circRNA loci in crops.

ACS Style

Jianping Zhou; Mingzhu Yuan; Yuxin Zhao; Quan Quan; Dong Yu; Han Yang; Xu Tang; Xuhui Xin; Guangze Cai; Qian Qian; Yiping Qi; Yong Zhang. Efficient deletion of multiple circle RNA loci by CRISPR‐Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice. Plant Biotechnology Journal 2021, 19, 1240 -1252.

AMA Style

Jianping Zhou, Mingzhu Yuan, Yuxin Zhao, Quan Quan, Dong Yu, Han Yang, Xu Tang, Xuhui Xin, Guangze Cai, Qian Qian, Yiping Qi, Yong Zhang. Efficient deletion of multiple circle RNA loci by CRISPR‐Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice. Plant Biotechnology Journal. 2021; 19 (6):1240-1252.

Chicago/Turabian Style

Jianping Zhou; Mingzhu Yuan; Yuxin Zhao; Quan Quan; Dong Yu; Han Yang; Xu Tang; Xuhui Xin; Guangze Cai; Qian Qian; Yiping Qi; Yong Zhang. 2021. "Efficient deletion of multiple circle RNA loci by CRISPR‐Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice." Plant Biotechnology Journal 19, no. 6: 1240-1252.

Journal article
Published: 11 January 2021 in The Plant Cell
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The regulated nucleocytoplasmic exchange of macromolecules is essential for the eukaryotic cell. However, nuclear transport pathways defined by different nuclear transport receptors (NTRs), including importins and exportins, and their significance in activating distinct stress responses are poorly understood in plants. Here, we exploited a CRISPR/Cas9-based genetic screen to search for modifiers of CONSTITUTIVE EXPRESSION OF PATHOGENESIS-RELATED GENE 5 (cpr5), an Arabidopsis thaliana nucleoporin mutant that activates autoimmune responses that partially mimic effector-triggered immunity (ETI). We identified an NTR gene, Exportin-4 (XPO4), as a genetic interactor of CPR5. The xpo4 cpr5 double mutant activates catastrophic immune responses, which leads to seedling lethality. By leveraging the newly developed proximity-labeling proteomics, we profiled XPO4 substrates and identified TOPLESS (TPL) and TPL-related (TPR) transcription corepressors as XPO4-specific cargo. TPL/TPRs target negative regulators of immunity and are redundantly required for ETI induction. We found that loss-of-XPO4 promotes the nuclear accumulation of TPL/TPRs in the presence of elevated salicylic acid (SA), which contributes to the SA-mediated defense amplification and potentiates immune induction in the cpr5 mutant. We showed that TPL and TPRs are required for the enhanced immune activation observed in xpo4 cpr5 but not for the cpr5 single-mutant phenotype, underscoring the functional interplay between XPO4 and TPL/TPRs and its importance in cpr5-dependent immune induction. We propose that XPO4 coordinates the nuclear accumulation of TPL/TPRs, which plays a role in regulating SA-mediated defense feedback to modulate immune strength downstream of CPR5 during ETI induction.

ACS Style

Feifei Xu; Min Jia; Xin Li; Yu Tang; Keni Jiang; Jinsong Bao; Yangnan Gu. Exportin-4 coordinates nuclear shuttling of TOPLESS family transcription corepressors to regulate plant immunity. The Plant Cell 2021, 33, 697 -713.

AMA Style

Feifei Xu, Min Jia, Xin Li, Yu Tang, Keni Jiang, Jinsong Bao, Yangnan Gu. Exportin-4 coordinates nuclear shuttling of TOPLESS family transcription corepressors to regulate plant immunity. The Plant Cell. 2021; 33 (3):697-713.

Chicago/Turabian Style

Feifei Xu; Min Jia; Xin Li; Yu Tang; Keni Jiang; Jinsong Bao; Yangnan Gu. 2021. "Exportin-4 coordinates nuclear shuttling of TOPLESS family transcription corepressors to regulate plant immunity." The Plant Cell 33, no. 3: 697-713.

Preprint content
Published: 05 January 2021
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Background: Rice plants suffer from the rising temperature which is becoming more and more prominent. Mining heat-resistant genes and applying them to rice breeding is a feasible and effective way to solve the problem.Result: Three main biomass traits, including shoot length, dry weight, and fresh weight, changed after abnormally high temperature treatment in the rice seedling stage of a recombinant inbred lines and germplasm population. Based on a comparison of the results of linkage analysis and genome-wide association analysis, two loci with lengths of 57 kb and 69 kb in qDW7 and qFW6, respectively, was associated with the rice response to abnormally high temperatures in the seedling stage. Meanwhile, based on integrated transcriptome analysis, some genes are considered as important candidate genes. Combining with known genes and analysis of homologous genes, it was found that there are eight genes in candidate intervals that need to be focused in subsequent research.Conclusions: The results indicated several relevant loci, which would help researchers to further discover beneficial heat-resistant genes that can be applied to rice heat-resistant breeding.

ACS Style

Zhaoran Wei; Qiaoling Yuan; Hai Lin; Xiaoxia Li; Chao Zhang; Hongsheng Gao; Bin Zhang; Huiying He; Tianjiao Liu; Jie Zhang; Xu Gao; Shandang Shi; Bo Wang; Zhenyu Gao; Lingrang Kong; Qian Qian; Lianguang Shang. Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress. 2021, 1 .

AMA Style

Zhaoran Wei, Qiaoling Yuan, Hai Lin, Xiaoxia Li, Chao Zhang, Hongsheng Gao, Bin Zhang, Huiying He, Tianjiao Liu, Jie Zhang, Xu Gao, Shandang Shi, Bo Wang, Zhenyu Gao, Lingrang Kong, Qian Qian, Lianguang Shang. Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress. . 2021; ():1.

Chicago/Turabian Style

Zhaoran Wei; Qiaoling Yuan; Hai Lin; Xiaoxia Li; Chao Zhang; Hongsheng Gao; Bin Zhang; Huiying He; Tianjiao Liu; Jie Zhang; Xu Gao; Shandang Shi; Bo Wang; Zhenyu Gao; Lingrang Kong; Qian Qian; Lianguang Shang. 2021. "Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress." , no. : 1.

Journal article
Published: 16 December 2020 in Rice Science
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Cadmium (Cd) is a non-essential toxic metal that is harmful to plants. To investigate the genetic mechanism of Cd tolerance in rice, quantitative trait loci (QTLs) associated with Cd tolerance at the seedling stage were analyzed using a recombinant inbred line (RIL) population derived from a cross between PA64s and 93-11. A total of 36 QTLs associated with shoot length, root length, shoot dry weight, root dry weight and total dry weight were detected in Hangzhou and Lingshui of China. Among them, 15 QTLs were identified under the control condition and 15 QTLs were identified under the Cd stress condition, and 6 QTLs for Cd tolerant coefficient were detected on chromosomes 1, 3, 7 and 9. The qCDSL1.1 and qCDSL1.2 were identified in Hangzhou and Lingshui, respectively, and had overlapping intervals on chromosome 1. To further confirm the effects of qCDSL1.1 and qCDSL1.2, we developed a chromosome segment substitution line (CSSL), CSSLqCDSL1, in 93-11 background harboring qCDSL1.1/qCDSL1.2 from PA64s. Compared to 93-11, CSSLqCDSL1 had increased shoot length under the Cd stress condition. These results pave the way for further isolation of those genes controlling Cd tolerance in rice and marker-assistant selection of rice elite varieties with Cd tolerance.

ACS Style

Ding Shilin; Liu Chaolei; Shang Lianguang; Yang Shenglong; Zhang Anpeng; Jiang Hongzhen; Ruan Banpu; Fang Guonan; Tian Biao; Ye Guoyou; Guo Longbiao; Qian Qian; Gao Zhenyu. Identification of QTLs for Cadmium Tolerance During Seedling Stage and Validation of qCDSL1 in Rice. Rice Science 2020, 28, 81 -88.

AMA Style

Ding Shilin, Liu Chaolei, Shang Lianguang, Yang Shenglong, Zhang Anpeng, Jiang Hongzhen, Ruan Banpu, Fang Guonan, Tian Biao, Ye Guoyou, Guo Longbiao, Qian Qian, Gao Zhenyu. Identification of QTLs for Cadmium Tolerance During Seedling Stage and Validation of qCDSL1 in Rice. Rice Science. 2020; 28 (1):81-88.

Chicago/Turabian Style

Ding Shilin; Liu Chaolei; Shang Lianguang; Yang Shenglong; Zhang Anpeng; Jiang Hongzhen; Ruan Banpu; Fang Guonan; Tian Biao; Ye Guoyou; Guo Longbiao; Qian Qian; Gao Zhenyu. 2020. "Identification of QTLs for Cadmium Tolerance During Seedling Stage and Validation of qCDSL1 in Rice." Rice Science 28, no. 1: 81-88.