Nanosecond electric pulses have several potential advantages in electroporation-based procedures over the conventional micro- and millisecond pulses including low level of heating, reduced electrochemical reactions and reduced muscle contractions making them alluring for use in biomedicine and food industry. The aim of this study was to evaluate if nanosecond pulses can enhance the cytotoxicity of chemotherapeutics bleomycin and cisplatin in vitro and to quantify metal release from electrodes in comparison to 100 μs pulses commonly used in electrochemotherapy. The effects of nanosecond pulse parameters (voltage, pulse duration, number of pulses) on cell membrane permeabilization, resealing and on cell survival after electroporation only and after electrochemotherapy with bleomycin and cisplatin were evaluated on Chinese hamster ovary cells. Application of permeabilizing nanosecond pulses in combination with chemotherapeutics resulted in successful cell kill. Higher extracellular concentrations of bleomycin - but not cisplatin - were needed to achieve the same decrease in cell survival with nanosecond pulses as with eight 100 μs pulses, however, the tested bleomycin concentrations were still considerably lower compared to doses used in clinical practice. Decreasing the pulse duration from microseconds to nanoseconds and concomitantly increasing the amplitude to achieve the same biological effect resulted in reduced release of aluminum ions from electroporation cuvettes.

The development of ruthenium-based complexes for cancer treatment requires a variety of pharmacological studies, one of them being a drug’s binding kinetics to serum proteins. In this work, speciation analysis was used to study kinetics of ruthenium-based drug candidates with human serum proteins. Two ruthenium (Ru) complexes, namely [(η⁶-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)Cl] (1) and [(η⁶-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)pta]PF₆ (2) (where pta = 1,3,5-triaza-7-phosphaadamantane), were selected. Before a kinetics study, their stability in relevant media was confirmed by nuclear magnetic resonance (NMR). Conjoint liquid chromatography (CLC) monolithic column, assembling convective interaction media (CIM) protein G and diethylamino (DEAE) disks, was used for separation of unbound Ru species from those bound to human serum transferrin (Tf), albumin (HSA) and immunoglobulins G (IgG). Eluted proteins were monitored by UV spectrometry (278 nm), while Ru species were quantified by post-column isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS). Binding kinetics of chlorido (1) and pta complex (2) to serum proteins was followed from 5 min up to 48 h after incubation with human serum. Both Ru complexes interacted mainly with HSA. Complex (1) exhibited faster and more extensive interaction with HSA than complex (2). The equilibrium concentration for complex (1) was obtained 6 h after incubation, when about 70% of compound was bound to HSA, 5% was associated with IgG, whereas 25% remained unbound. In contrast, the rate of interaction of complex (2) with HSA was much slower and less extensive and the equilibrium concentration was obtained 24 h after incubation, when about 50% of complex (2) was bound to HSA and 50% remained unbound.

Due to its fibrous structure and high water holding capacity, rock mineral wool (RMW) has boosted the development of hydroponics. Consequently, the amount of waste RMW has also increased tremendously, which has stimulated the research and development of RMW reuse options. In this study, composting and degradability of RMW from hydroponics (gRMW) were tested in combination with different ratios of biowaste compost, including physical and chemical properties of the starting and final materials, and potential ecological hazards of the final product. gRMW had high water holding capacity and low organic matter content, which was easily degradable. Limits of toxic elements according to EU regulation were not exceeded. Degraded gRMW mixtures with compost did not exhibit toxicity to plants or aquatic bacteria and showed intermediate or limited habitat function for earthworms, which preferred the sole gRMW not mixed with compost. Overall, degraded gRMW exhibited parameters of safe soil amendment.

The uptake and translocation of Pb, Cr, Cd, and Zn in tomato plants (Solanum lycopersicum L. Rally) were investigated. Tomato seedlings were grown for five weeks in pots containing 40 L of Hoagland nutrient solution (pH 7) or contaminated nutrient solutions at two concentration levels for each element: Cr (100 and 1000 ng/mL), Zn (100 and 1000 ng/mL), Pb (100 and 500 ng/mL), and Cd (50 and 500 ng/mL). The solutions were replenished weekly to maintain a volume of 40 L (pH 7), and 10 mL samples were collected for elemental analysis. After five weeks, the plants were harvested and separated into roots, stems, leaves, and fruits. These samples underwent microwave-assisted digestion, and the element concentrations were determined by inductively coupled plasma mass spectrometry (ICP-MS). The results revealed that the elements were mainly accumulated in the roots, with much lower concentrations determined in the fruits. Pb and Cr accumulated only minimally in fruits, with Pb levels of 0.0009 mg/kg wet weight at LI and 0.003 mg/kg wet weight at LII, and Cr levels of 0.028 mg/kg wet weight at LI and 0.031 mg/kg wet weight at LII. The Pb levels did not exceed the permissible limits set by EC regulations (0.05 mg/kg wet weight). Zn exhibited the highest accumulation in fruits, with 2.17 mg/kg wet weight at LI and 4.8 mg/kg wet weight at LII. By contrast, the Cd concentrations in fruits (0.25 mg/kg wet weight at LI and 1.1 mg/kg wet weight at LII) exceeded the EC regulatory limit of 0.02 mg/kg wet weight. The uptake of other essential elements into the tomato plant remained largely unaffected by the presence of contaminants. These results provide valuable insights into food safety. Laser ablation (LA)-ICP-MS imaging revealed an even distribution of Cd and Zn in the leaves of plants grown in contaminated nutrient solutions. By contrast, Cr and Pb were predominantly localized in the leaf veins and at the leaf apex, suggesting different transport mechanisms for these elements from the roots to the aerial parts of the plant.