Plants are routinely subjected simultaneously to different abiotic and biotic stresses, such as heat, drought, and insect infestation. Plant–insect interactions in such complex stress situations are poorly understood. We evaluated the performance of the grain aphid (Sitobion avenae) in wheat (Triticum aestivum L.) exposed to a combination of heat and drought stresses. We also performed assays of the relative water content, nutritional quality, and responses of phytohormone signaling pathways. Lower relative water content and accumulation of soluble sugars and amino acids were observed in plants exposed to combined heat and drought stress. These conditions increased abscisic acid levels in the absence of aphids, as well as leading to higher levels of jasmonate-dependent transcripts. The grain aphid infestation further increased abscisic acid levels and the abundance of jasmonic acid- and salicylic acid-dependent defenses under the combined stress conditions. Aphids reared on plants grown under drought stress alone showed lower net reproductive rates, intrinsic rates of increase, and finite rates of increase compared with aphids reared on plants in the absence of stress. The heat-treated plants also showed a decreased aphid net reproductive rate. These findings demonstrate that exposure to a combination of stresses enhances plant defense responses against aphids as well as altering nutritional quality.

Arbuscular mycorrhizal fungi (AMF) can increase host plant nutrient uptake via their mycelium, thus promoting plant growth. AMF have always been associated with successful invasion of most exotic plant species. However, knowledge regarding how AMF affect the success of plant invasion remains limited. Exotic Ambrosia artemisiifolia is an invasive and mycorrhizal plant species. A long-term field experiment was conducted to examine the differences in AMF diversity and composition in the roots of A. artemisiifolia and Setaria viridis subjected to interspecific competition during growth. A greenhouse experiment was also performed to test the effect of Funneliformis mosseae on the growth of these two species. Ambrosia artemisiifolia invasion caused AMF diversity to change in native S. viridis roots. Meanwhile, the relative abundance of F. mosseae was significantly higher in the roots of A. artemisiifolia than in those of S. viridis. The higher AMF colonization rate in the exotic species (A. artemisiifolia) than in the native species (S. viridis) was found in both the field and greenhouse experiments. The greenhouse experiment possibly provided that AMF advantaged to the growth of A. artemisiifolia, by influencing its photosynthetic capacity as well as its phosphorus and potassium absorption. These observations highlight the important relationship of AMF with the successful invasion of A. artemisiifolia.

Frankliniella occidentalis and F. intonsa are devastating pest insects that target Rosa rugosa, Chrysanthemum morifolium, and Phaseolus vulgaris, which are important economical horticultural plants in China. Meanwhile, R. rugosa and C. morifolium are important cash plants in Kunming, South China. We focus on the population performance of these two thrips species on these three host plants with or without repeated exposure to imidacloprid in Kunming. In the field, the population numbers of F. occidentalis developed faster and were larger on these three sampled host plants, especially under imidacloprid exposure, compared with F. intonsa. The activity of the detoxifying enzymes (CarE, AchE, and MFO) and the antioxidant enzymes (CAT and POD) in both thrips species were significantly enhanced under imidacloprid exposure, whereas the activities of SOD in both thrips were significantly decreased on these three host plants, compared with the control. Overall, enzyme activity of F. occidentalis showed a greater increase than that observed in F. intonsa in most cases, which could be exploited in further studies on thrips resistance management.

Bacillus can help plants to acquire nutrients either directly or indirectly. However, the role of Bacillus community on the competitive growth of invasive Ambrosia artemisiifolia is poorly understood. Native Setaria viridis is often found in areas that have been invaded by A. artemisiifolia. We sought to determine whether the quantitative and/or qualitative differences in the Bacillus community present on the invasive A. artemisiifolia and native S.viridis provide a competitive advantage to the invasive over native species. A field experiment was established to imitate the invasion of A. artemisiifolia. The 16S rRNA gene was commercially sequenced to identify the bacilli isolated from the rhizosphere soil of field-grown A. artemisiifolia and S. viridis. The Bacillus communities in their rhizosphere were compared, and their effects on the competitive growth of A. artemisiifolia and S. viridis were tested in the pot experiments. Bacillus in the rhizosphere soil of A. artemisiifolia significantly enhanced its intra-specific competitive ability. The relative abundance of B. megaterium in the rhizosphere soil of A. artemisiifolia was significantly higher than that of S. viridis. Inoculation with B. megaterium that was isolated from the rhizosphere soil of both A. artemisiifolia and S. viridis significantly enhanced the relative competitiveness of A. artemisiifolia and inhibited that of S. viridis. The higher abundance of B. megaterium in the rhizosphere of A. artemisiifolia creates higher levels of available nutrients than that in the native S. viridis, which enhance the competitive growth of A. artemisiifolia. The result helps to discover the mechanism of Bacillus community in the invasion of A. artemisiifolia.