The Argentine ant (Linepithema humile) is recognized as one of the world's most damaging invasive species. One reason for the ecological dominance of introduced Argentine ant populations is their ability to dominate food and habitat resources through the rapid mobilization and recruitment of thousands of workers. More than 30 years ago, studies showed that (Z)-9-hexadecenal strongly attracted Argentine ant workers in a multi-choice olfactometer, suggesting that (Z)-9-hexadecenal might be the trail pheromone, or a component of a trail pheromone mixture. Since then, numerous studies have considered (Z)-9-hexadecenal as the key component of the Argentine ant trails. Here, we report the first chemical analyses of the trails laid by living Argentine ants and find that (Z)-9-hexadecenal is not present in a detectible quantity. Instead, two iridoids, dolichodial and iridomyrmecin, appear to be the primary chemical constituents of the trails. Laboratory choice tests confirmed that Argentine ants were attracted to artificial trails comprised of these two chemicals significantly more often than control trails. Although (Z)-9-hexadecenal was not detected in natural trails, supplementation of artificial dolichodial+iridomyrmecin trails with an extremely low concentraion of (Z)-9-hexadecenal did increase the efficacy of the trail-following behavior. In stark contrast with previous dogma, our study suggests that dolichodial and iridomyrmecin are major components of the Argentine ant trail pheromone. (Z)-9-hexadecenal may act in an additive manner with these iridoids, but it does not occur in detectable quantities in Argentine ant recruitment trails.

Current control measures for Argentine ants, Linepithema humile (Mayr), in urban settings typically include perimeter applications of insecticides around structures, resulting in potential problems with insecticide runoff and environmental contamination. Insecticidal baits can be an effective alternative to perimeter spray applications and are largely considered target-specific with minimal nontarget impact and environmental contamination. We report a “pheromone-assisted baiting technique” as an economically viable approach to maximize the efficacy of conventional baits targeting Argentine ants. Laboratory experiments with a commercially available gel bait indicated that foraging activity and final mortality of Argentine ants were significantly improved by incorporating (Z)-9-hexadecenal in the bait. The field study demonstrated that the pheromone-treated gel bait achieved a 74% reduction in Argentine ant activity by the end of 4 wk when it was compared with its own pretreatment value. This was a significant improvement over the untreated gel bait that provided a 42% reduction over the same period of time. The pheromone-assisted baiting technique has the potential in providing effective ant control with reduced amount of insecticides applied in the environment.

Gel bait insecticides have been extensively used to manage the German cockroach, Blattella germanica (L.) (Blattodea: Ectobiidae), but issues with reduced effectiveness of such formulations are becoming increasingly common. We collected five field strains of German cockroaches in California and evaluated them against five commercial bait products [Maxforce FC Magnum (0.05% fipronil), Maxforce Impact (1% clothianidin), Advion Evolution (0.6% indoxacarb), Optigard (0.1% emamectin benzoate) and Siege (2% hydramethylnon)]. Increased survivorship and incomplete mortality towards all baits were recorded in the field strains. We assessed susceptibility to the active ingredients fipronil, clothianidin, indoxacarb, abamectin, hydramethylnon, and deltamethrin using topical bioassays with diagnostic doses (3 × LD95 and 10 × LD95) developed from the UCR susceptible strain. Low mortality was registered when tested with the 3 × LD95’s of deltamethrin (0%), fipronil (0–3%), and clothianidin (13–27%); low to moderate mortality when treated with the 3 × LD95 of indoxacarb (13–63%), and moderate to high mortality after treatment with the 3 × LD95 of abamectin (80–100%) and hydramethylnon (70–83%). The mortality of all strains remained low after treatment with the 10 × LD99 of deltamethrin (0–20%) and low to moderate with fipronil (20–70%). We found negative correlations (P < 0.05) between Advion Evolution mean survival time and indoxacarb 10 × LD95 mortality and between Maxforce Impact and clothianidin 10 × LD95 mortality. These findings demonstrate multiple resistance towards all tested commercial bait insecticides except Optigard, suggesting the effectiveness of avermectin products in resistance management programs.

Fresh and aged residual deposits of several insecticide products were tested against bed bug adults to determine if a recent bloodmeal affected their