The Transbaikal zokor (Myospalax psilurus) is a dominant rodent distributed in the meadow steppe of Inner Mongolia in northern China. Due to long history of evolution in subterranean environment, the zokor has an adaptive behavior: sealing burrow entrances. When a burrow is damaged, exposed entrances appear, and within a relatively short time, the zokor would be active in sealing the entrances to reduce risks to its survival. In general, it is thought that zokors avoid light and wind, which is consistent with their behavior of sealing burrow entrances. However, direct evidence from field experimental research has been lacking. This study set up 68 field sampling points in a meadow steppe in Inner Mongolia from August to September, 2014 and used a wind–light isolator to study the effects of wind and light factors on zokor burrow entrance sealing behavior. The results showed that there were no significant correlations between wind or light factors and the frequency of zokor burrow entrance sealing. Therefore, wind and light factors are not direct factors associated with zokors actively sealing burrow entrances.

Grazing by large herbivores can potentially affect interspecific interactions between small herbivores by reducing the ecological fitness of animals. Desert rodents are important components in desert ecosystems and indicators of environmental change. Grazing reduces food resources, but rodents can decrease interspecific niche overlap by adaptive behavior. However, the key factors driving rodent behavioral activities and coexistence in the Alxa desert remains unstudied. We monitored population density and behavioral activities of Midday gerbil (Meriones meridianus) and northern three-toed jerboa (Dipus sagitta) in a grazing exclusion experiment in Alxa desert, Inner Mongolia, China, in 2017. We assessed the relationship between environmental factors (such as plant height, density, coverage, rainfall and temperature) and the behavioral activities of two coexisting rodent species. The results showed that: (1) In summer, grazing significantly reduced the activity time of gerbil and jerboa compared to that in grazing exclusion areas (gerbil: F = 5.98, p < 0.05, η² = 0.22; jerboa: F = 8.57, p < 0.01, η² = 0.28). Grazing reduced the temporal niche overlap with an obvious shifting of activity peaks between two species. (2) Grazing exclusion enhanced the temporal niche overlap between the two rodent species due to greater food availability which relieved inter-specific competition in each season. (3) Grazing strengthened the sensitivity of rodents to environmental changes in all seasons. These results indicated that grazing affected competition between the rodent species by altering vegetation conditions, which in turn affected the temporal niche and activity patterns of rodents.

The objective of this study was to investigate the effects of grazing on midday gerbil (Meriones meridianus) population characteristics and survival of animals of different genders. The experiment used a randomized complete block design and was conducted in Alxa Left Banner, Inner Mongolia, China, in 2002 (The agricultural reclamation plots set up in 1994). From April 2006 to October 2010, midday gerbils were live-trapped in 3 light grazing plots, 3 overgrazed plots, and 3 grazing exclusion plots. The quantity of vegetation was investigated in the two different grazing intensity areas and grazing exclusion area to determine the relationship between gerbils and plant food availability. The results suggested that there was higher gerbil density, individual body mass, and daily body mass growth rate in the grazing exclusion sites than the other sites across the whole year. Females had higher survival in grazing exclusion areas than in other treatments, but the males’ survival showed the opposite pattern. Our results indicated that grazing negatively influenced the midday gerbil population by reducing food availability. Grazing influenced the survival rates of male midday gerbils positively, but had negative effects on females. The reason for gendered differences in survival rates of midday gerbils requires further investigation.

As the most abundant group of mammals, rodents possess a very rich ecotype, which makes them ideal for studying the relationship between diet and host gut microecology. Zokors are specialized herbivorous rodents adapted to living underground. Unlike more generalized herbivorous rodents, they feed on the underground parts of grassland plants. There are two species of the genus Myospalax in the Eurasian steppes in China: one is Myospalax psilurus, which inhabits meadow grasslands and forest edge areas, and the other is M. aspalax, which inhabits typical grassland areas. How are the dietary choices of the two species adapted to long-term subterranean life, and what is the relationship of this diet with gut microbes? Are there unique indicator genera for their gut microbial communities? Relevant factors, such as the ability of both species to degrade cellulose, are not yet clear. In this study, we analyzed the gut bacterial communities and diet compositions of two species of zokors using 16S amplicon technology combined with macro-barcoding technology. We found that the diversity of gut microbial bacterial communities in M. psilurus was significantly higher than that in M. aspalax, and that the two species of zokors possessed different gut bacterial indicator genera. Differences in the feeding habits of the two species of zokors stem from food composition rather than diversity. Based on the results of Mantel analyses, the gut bacterial community of M. aspalax showed a significant positive correlation with the creeping-rooted type food, and there was a complementary relationship between the axis root-type-food- and the rhizome-type-food-dominated (containing bulb types and tuberous root types) food groups. Functional prediction based on KEGG found that M. psilurus possessed a stronger degradation ability in the same cellulose degradation pathway. Neutral modeling results show that the gut flora of the M. psilurus has a wider ecological niche compared to that of the M. aspalax. This provides a new perspective for understanding how rodents living underground in grassland areas respond to changes in food conditions.

False zokor (Myospalax aspalax) is one of the subterranean rodents distributed in East Asia. Molecular data and demography of M. aspalax are unknown, and the population management is difficult, because it is specialized for underground life. In this study, the complete mitochondrial DNA genome of M. aspalax was determined. The results suggested that the mitogenome was 16,436 bp in length, and consisted of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one displacement loop. The overall base composition of the heavy strand was A 33.40%, T 29.34%, C 24.90%, and G 12.36%, with A + T > C + G. Myospalax aspalax is a sister to the genus Myospalax, and have a most closely relationship with M. psilurus. The mitogenome data are potentially important for population management of M. aspalax and should contribute to the interspecies comparative analyses of genus Myospalax.