Laropal^® A81 is a urea-aldehyde resin used as a varnish for paintings requiring 30%–40% of aromatic solvents to be dissolved. Considering the dangers of aromatic solvents for the environment and health, an attempt was made to replace them with less impactful and low-toxic solvents. The present research investigates a new formulation with aliphatic hydrocarbons and esters (isobutyl isobutyrate, IBIB) as an alternative to traditional aromatics mixture. Traditional and alternative varnish formulations of Laropal^® A81 were studied on an inert support and paint samples to test the resin by itself and in interaction with paint film, respectively. This study aims to compare the two different formulations of Laropal^® A81 by evaluating their optical, colorimetric, and stability proprieties before and after natural and accelerated ageing. Colorimetry and spectroscopic techniques (transmittance and reflectance UV-VIS-NIR, FORS, transmittance and total reflectance FT-IR, and ¹H-NMR) were used for the assessment. Very promising results have been obtained with the application of the alternative formulation, with data comparable to those of the traditional formulation, paving the way for the replacement of the aromatic-based solvents traditionally used with IBIB. This allows a safer and more sustainable conservation practice with considerable benefits for the health of the operators and the environment.