Except for transpired water, the intracellular water stored in leaves accounts for only 1–3% of the water absorbed by roots. Understanding water transport and use, as well as the related photosynthetic response, helps with determining plant water status and improving the revegetation efficiency in fragile karst habitats. In this study, we conducted experiments on 8 year old naturally growing plants of Coriaria nepalensis Wall., Broussonetia papyrifera (L.) Vent., and Elaeocarpus decipiens Hemsl. in karst areas. We determined the diurnal variations in leaf electrophysiology, water potential, gas exchange, and chlorophyll fluorescence parameters. The results indicated that C. nepalensis plants maintained a high photosynthetic rate, with a high root water uptake ability and leaf intracellular water-holding capacity (LIWHC). The stomata quickly closed to conserve water within cells and protect the photosynthetic structure. B. papyrifera maintained stable intracellular water transport rate (LIWTR), and the photosynthetic efficiency was increased with increasing intracellular water-use efficiency (LIWUE). B. papyrifera also maintained its photosynthesis by efficiently using the transpired water when the LIWHC was increased. The inter- and intracellular water in the leaves of E. decipiens remained stable, which could be attributed to the leathery leaves and its high water-holding capacity. The photosynthesis of E. decipiens was low and stable. Compared with the high photosynthesis, high transpiration, and low instantaneous water-use efficiency (WUE_i) pattern in C. nepalensis plants, E. decipiens plants exhibited low photosynthesis, low transpiration, and low WUE_i, whereas B. papyrifera plants presented high photosynthesis, low transpiration, and high WUE_i. Plants in karst regions change their transport and use of intracellular leaf water to regulate the photosynthetic performance, which differs among different plant species.

Orychophragmus violaceus (L.) O. E. Schulz adapts to karst environments through a variety of adaptability mechanisms. However, the leaf intracellular water translocation and utilization mechanism is still unknown. This study hypothesizes that plants adapt to dehydration by synergistically adjusting the leaf anatomy, cell elasticity and intracellular water translocation. Leaf structure, elastic modulus (Em), physiological capacitance (CP), impedance (Z), water potential (Ψ_L), leaf tensity (LT) and chlorophyll fluorescence parameters of the detached leaves in plants of O. violaceus and Brassica napus L. were measured at each water loss time (0, 1, 2, 3, 4 and 5 h). The uniform leaves were randomly selected from five different plants for each species. The cell vacuole volume and translocation resistance of intracellular water could be represented by the electrophysiological parameters, such as CP and Z. The results indicated that timely shrinkage of O. violaceus leaves and mesophyll cells together with the increased water translocation resistance retained the intracellular water and maintained the turgor pressure. Water within sponge parenchyma could also be translocated into palisade parenchyma. The PSII reaction center was kept stable, and the photosynthetic activity of O. violaceus was clearly inhibited at 3 h. Palisade parenchyma of B. napus leaves increased quickly to improve the intercellular water translocation due to the strong cell stiffness. Gradually increasing intracellular water translocation resistance and recovery of the cell elasticity slowed down the leaf water loss, which, however, could not timely stop the damage on the PSII reaction center and the photochemical efficiency. The photochemical efficiency was seriously inhibited at 4 h and 5 h. The response mechanism of intracellular water to dehydration can be investigated with the help of leaf electrophysiological traits. However, the direct determination of plant drought resistance using electrophysiological information can still not be realized at present and needs further research.

Exogenous application of plant hormones has been considered a short-term and effective strategy to alleviate deleterious effects of water stress on plants. However, whether exogenous gibberellic acid (GA₃) directly enhances nitrogen accumulation and thereby alleviates drought stress in soybean (Glycine max (Linn.) Merr.) remains to be investigated. This study set three water treatments (75% CK, 50% MD, 25% SD), with half of the plants at each level sprayed with 10⁻⁶ mol·L⁻¹ GA₃, measuring growth, photosynthesis, nitrogen content, water status, and electrophysiological parameters and calculating cellular metabolic electronic energy (ΔG_B) based on Nernst equation. The results showed that drought reduced soybean nitrogen accumulation, photosynthesis, growth and yield. GA₃ increased soybean nitrogen accumulation, improving photosynthesis and yield under CK, which enhanced the consumption of intracellular stored energy and reduced ΔG_B. Under MD, GA₃ improved leaf water status, promoted soybean nitrogen accumulation and photosynthesis and reduced ΔG_B by allocating more energy to drought resistance; it could therefore mitigate the moderate drought stress on plants. ΔG_B negatively correlated with total nitrogen content and yield, indicating that ΔG_B was a potential indicator associated with nitrogen accumulation, which can guide the optimization of GA₃ spraying strategies. Further studies on GA₃ application details are necessary to improve the soybean yields under drought conditions.