Potatoes rank third in terms of human consumption after rice and wheat. Globodera spp. are significant pests of potato crop worldwide. Globodera rostochiensis was found in Weining County, Guizhou Province, China, in 2019. We collected soil from the rhizosphere zone from infected potato plants and separated mature cysts through simple floatation and sieving methods. The selected cysts were surface-sterilized, and the colonized fungi were isolated and purified. At the same time, the preliminary identification of fungi and fungi parasites on the cysts of nematodes was carried out. This study aimed to define the species and frequency of fungi-colonizing cysts of G. rostochiensis collected from Weining County, Guizhou Province, China, and provide a basis for the control of G. rostochiensis. As a result, 139 strains of colonized fungi were successfully isolated. Multigene analyses showed that these isolates included 11 orders, 17 families, and 23 genera. The genera Fusarium (with a separation frequency of 59%), Penicillium (11%), Edenia (3.6%), and Paraphaeosphaeria (3.6%) were the most frequently occurring. Among the 44 strains, 27 had a colonization rate of 100% on the cysts of G. rostochiensis. Meanwhile, the functional annotation of 23 genera indicated that some fungi have multitrophic lifestyles combining endophytic, pathogenic, and saprophytic behavior. In conclusion, this study showed the species composition and lifestyle diversity of colonized fungi from G. rostochiensis and demonstrated these isolates as potential sources of biocontrol agents. Colonized fungi were isolated from G. rostochiensis for the first time in China, and the taxonomic diversity of fungi from G. rostochiensis was clarified.

N⁶-methyladenosine (m⁶A) is a dynamic post-transcriptional RNA modification. Recently, its role in viruses has led to the study of viral epitranscriptomics. m⁶A has been observed in viral genomes and alters the transcriptomes of both the host cell and virus during infection. The effects of m⁶A modifications on host plant mRNA can either increase the likelihood of viral infection or enhance the resistance of the host to the virus. However, to date, the regulatory mechanisms of m⁶A in viral infection and host immune responses have not been fully elucidated. With the development of sequencing-based biotechnologies, the study of m⁶A in plant viruses has received increasing attention. In this mini review, we summarize the positive and negative consequences of m⁶A modification in different RNA viral infections. Given its increasingly important roles in multiple viruses, m⁶A represents a new potential target for antiviral defense.