Aerobic vaginitis (AV) is a recently defined vaginal recurring infection, which is treated with antibiotics. However, excessive and prolonged use of antibiotics disrupts healthy vaginal microflora and leads to the emergence of antibiotic resistance among pathogens. This situation has directed researchers to explore alternative antimicrobials. The current study describes in vitro and in vivo antimicrobial efficacy and pharmaceutical interactions between plant essential oils (EOs) and five lactic acid bacteria (LABs), isolated from the healthy vagina, against E. faecalis, one of the major etiological agents of AV. In vitro experiments confirm good antimicrobial activity of both plant EOs and cell free supernatant (CFS) from LABs. Based on high antimicrobial efficacy, Moringa essential oil (MO) was selected to determine its nature of interaction with CFS of five LAB strains. Synergism was recorded between MO and CFS of L. reuteri (MT180537). To validate in vitro findings, prophylactic responses of individual and synergistic application of MO and L. reuteri (MT180537) were evaluated in an E. faecalis (MW051601) induced AV murine model. The prophylactic efficacy was evidenced by a reduction in intensity of clinical symptoms, E. faecalis (MW051601) count per vaginal tissue along with a reduction in AV associated changes in histological markers of infection in animals receiving Moringa essential oil and L. reuteri (MT180537) alone or in combination. However, significant synergism between Moringa essential oil and L. reuteri (MT180537) could not be observed. Our data confirms the importance of in vivo experiments in deducing pharmacological interactions.

Background: The plant Murraya koenigii (L.) Spreng.native to Pakistan and India and a part of the Rutaceae family, plays a vital role in the Indian Ayurvedic medicine system. Studies have shown that this plant's bark, roots, and leaves contain many carbazole alkaloids. These alkaloids are known to have substantial therapeutic properties. Methods: The crushed and powdered organs of M. koenigii were extracted with several solvents (acetone, chloroform, and methanol). Then, using column chromatography, a dull brown oily substance, MK- 1 (1.05 g), was obtained with chloroform and methanol (7:3). Result We have successfully isolated a new carbazole alkaloid, 3-geranyl 8-hydroxy 6, 7-di methoxy 3’, 3’-dimethyl 1, 2-pyranocarbazole, from the seeds of M. koenigii. The structure was further elucidated by cross-referencing our NMR, UV, IR, and MS data with that found in the published literature. Conclusion: Comparing the previously published literature data clearly explained that this alkaloid is new to M. koenigii.

Expensive and ineffective feed ingredients in fish feed are becoming a major problem for fish growth in the aquaculture industry. Therefore, the experiment was conducted to investigate the utilization of fruit processing wastes as a dietary component in the feed of rohu (Labeo rohita). Four different feed formulations were prepared using commercial diet and supplemented with different levels of orange peel powder (CT= 0%, T1= 15%, T2= 30% and T3= 45%). The experiment was performed in aquariums of dimensions 1x1.5x2 feet, filled with approximately 79 liters of water. Fish were fed two times a day at rate of 3% of their body weight for 35 days. The results showed that the T1, T2, and T3 treatments had almost 104%, 160%, and 68% higher weight gain than that of CT, respectively. The T1 (7.55%), T2 (17.78%), and T3 (4.26%) treatments had a lower feed conversion ratio than that of CT. The T1, T2, T3 treatments had almost 14.18%, 15.27%, and 6.11% lower mean corpuscular hemoglobin concentration than that of CT. The T1 and T2 treatments had almost 7.01% and 17.42% higher total serum protein than that of CT, respectively. The T1 (0.90%) and T2 (6.23%) treatments had higher A/G ratios than that of CT, respectively and T3 (0.01%) had lower A/G ratio than that of CT. Overall, the dietary supplementation of 30% orange peel improved growth performance, feed utilization, oxidative stress, and hematological parameters in Labeo rohita.