Isomerization of aldoses to ketoses is an essential step in carbohydrate valorization routes in biorefineries to produce a wide variety of bioproducts. In this work, selective isomerization of aldoses into ketoses was investigated using different commercial Brønsted basic anion resins at low temperature conditions. Weak and strong basic resins were tested under different reaction conditions. Amberlite IRA-900 and Amberlyst A-26 (strong resins) and Amberlite IRA-67 and Amberlyst A-21 (weak resins) were tested to assess their catalytic properties. Strong basic resins provided high yields of fructose. IRA-900 was also tested in the isomerization of different sugar monosaccharides conventionally present in lignocellulosic biomass (xylose, arabinose, galactose, glucose and mannose) aiming to explore the performance of this material in hemicellulose-derived sugar mixtures. Very promising performance was observed for IRA-900, yielding fructose selectivity higher than 75% and fructose yield of 27% in the isomerization reaction. Notably, basic anionic resins were not suitable for reuse in different reaction cycles, although the use of organic cosolvents, specifically ethanol, improved the reusability of the tested resins.