Introduction Innate immune response (IIR) is the first line of defence of the body and is dedicated to the containment of the level of infections before the establishment of an active immune response (AIR). Innate immune response is an expeditious and

Antimicrobial resistance (AMR) is a major global concern in both human and veterinary medicine. Among antimicrobial resistance (AMR) bacteria, Extended-Spectrum Beta-Lactamases (ESBLs) pose a serious health risk because infections can be difficult to treat. These Gram-negative bacteria can be frequently found in poultry and in Italy, where such protein production is established. ESBL-producing Escherichia coli, Salmonella and Klebsiella in chicken and turkey may pose a significant public health risk due to potential transmission between poultry and humans. This review aims to assess the prevalence of ESBL-producing E. coli, Salmonella and Klebsiella phenotypically and genotypically in Italian poultry, identifying the most common genes, detection methods and potential information gaps. An initial pool of 1462 studies found in scientific databases (Web of Sciences, PubMed, etc.) was screened and 29 were identified as eligible for our review. Of these studies, 79.3% investigated both phenotypic and genotypic ESBL expression while ${bla}_{CTX-M}$, ${bla}_{TEM}$ and ${bla}_{SHV}$ were considered as targeted gene families. Large differences in prevalence were reported (0–100%). The ${bla}_{CTX-M-1}$ and ${bla}_{TEM-1}$ genes were the most prevalent in Italian territory. ESBL-producing E. coli, Salmonella and Klebsiella were frequently detected in farms and slaughterhouses, posing a potential threat to humans through contact (direct and indirect) with birds through handling, inhalation of infected dust, drinking contaminated water, ingestion of meat and meat products and the environment. Considering the frequent occurrence of ESBL-producing bacteria in Italian poultry, it is advisable to further improve biosecurity and to introduce more systematic surveillance. Additionally, the focus should be on the wild birds as they are ESBL carriers.

The poultry industry suffers from various diseases or stresses. In poultry, apart from being antipyretic, acetylsalicylic acid (ASA) is widely used to cope with different issues including heat stress, and respiratory and digestive orders. This study evaluated the beneficial and toxic effects of ASA at different dose levels in broiler birds. To evaluate these toxic and beneficial effects it is necessary to examine the physical and serum biochemical parameters as well as the histopathological changes with tissue sections taken from broiler birds under experimental trial. This study was conducted on 60 one-day-old broiler chicks purchased from a local market in Faisalabad. Chicks were reared for the first 14 days under similar conditions. On the 15th day, birds were randomly divided into 4 groups (1-4) with 15 birds in each group. Group 4 was kept as control, while groups 1, 2, and 3 were treated with ASA at the dose of 300, 600, and 1200 mg/L of drinking water for 21 days. There were 3 samplings performed at 21, 28, and 35 days post-treatment. The live body weight and carcass weight were noted on each sampling. All the visceral organs were recorded for gross pathological changes. The serum samples were collected for biochemical evaluation. Histopathology of all the visceral organs was performed to observe the microscopic changes. A significant (P<0.05) increase in live body weight at a 300mg/L dose was noted after the first 2 samplings. A significant (P<0.05) increase in the relative organ weight was recorded at 1200 mg/L. The groups treated with ASA 600 and 1200 mg/L showed increased (P<0.05) AST, ALT, and creatinine levels from that of the control group. The group treated with 1200 mg/L of ASA showed increased (P<0.05) urea, serum total protein, and albumin level in all the samplings. Histopathological changes revealed swollen hepatocytes, increased sinusoidal spaces in the liver, congestion and abnormal glomerular spaces in the kidney, congestion and alveolar disruption in the lungs, and generation of villi and cellular degeneration in the intestine in a high-dose group. The study concluded that ASA at a low dose can be used for a long time in broilers and has a growth promontory role, while high-level doses cause hepatorenal toxicity.

Necrotic enteritis is an important disease of poultry that causes economic loss to the broiler industry. Clostridium perfringens is an important bacterium that is responsible for causing necrotic enteritis. Antibiotics are mainly used to control C. perfringens but due to resistance antibiotics are banned in many countries like Canada, Hong Kong, and the European Union. Many alternatives such as probiotics, essential oils, and postbiotics have been developed to control C. perfringens. Among them, probiotics are very important because they can increase beneficial bacteria in the intestine, create a competitive environment in the gut region, and prevent the adhesion and colonization of pathogenic bacteria such as C. perfringens. Probiotics cause immune system modulation, reducing inflammatory markers such as cytokines. Lactobacillus based probiotics also cause weight gain, improve feed conversion ratio, and decrease mortality in poultry which in turn increase profit margin. Several studies have reported that when poultry populations were challenged with C. perfringens then these probiotics prevented intestinal lesions, provided anti-inflammatory effects to the intestine, prevented damage to the villi, and did not allow C. perfringens to form its colony in the intestine. The main aim of this review paper is to explain the updated information on necrotic enteritis, the damages caused to the gut, and the mechanism of actions through which Lactobacilli work against C. perfringens.