Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m² on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect.

Transcranial direct current stimulation (tDCS) is a noninvasive neural control technology that has become a research hotspot. To facilitate further research of tDCS, the biosafety of 500 μA cathodal tDCS, a controversial parameter in rats was evaluated. 24 animals were randomly divided into two groups: a cathodal tDCS group (tDCS, n = 12) and control group (control, n = 12). Animals in the tDCS group received 5 consecutive days of cathodal tDCS (500 μA, 15 min, once per day) followed by a tDCS-free interval of 2 days and 5 additional days of stimulation, totally two treatments of tDCS for a total of 10 days. Computational 3D rat model was adopted to calculate the current density distributions in brain during tDCS treatment. Essential brain functions including motor function and learning and memory ability were evaluated. Additionally, to estimate the neurotoxicity of tDCS, the brain morphology, neurotransmitter levels and cerebral temperature were investigated. Our results showed that the current density inside the brain was less than 20 A/m2 during tDCS treatment in computational model. tDCS did not affect motor functions and learning and memory ability after tDCS treatment. In addition, no significant differences were found for the tDCS group in hematology, serum biochemical markers or the morphology of major organs. Moreover, tDCS treatment had no effect on the brain morphology, neural structures, neurotransmitter levels or cerebral temperature. 500 μA cathodal tDCS as performed in the present study was safe for rodents.

The impact of electromagnetic radiation from communication on the male reproductive system has emerged as a significant concern in public health. A notable distinction of the 5G sub-6 GHz band, compared to traditional 2G, 3G, and 4G frequency bands, is the inclusion of higher frequency bands. This has raised public concerns regarding the potential effects of these higher frequencies on organisms, particularly their reproductive systems. While it is imperative to investigate the biological effects and potential risks associated with these new frequency bands in laboratory settings, comparing and evaluating differences between various frequency bands remain challenging due to the absence of standardized parameters such as exposure conditions and duration. In contrast, dose assessment offers a simpler and more reliable approach. The dose assessment method was employed in this study to investigate the risks associated with sub-6 GHz electromagnetic radiation from 5G base stations on the male reproductive system. A classical human body model (Duke) was utilized, and an electromagnetic simulation environment was established based on the actual polarization direction of the exposed base stations and various body postures. This research explored the effects of field direction, posture, public population, and frequency on the specific absorption rate of the reproductive system. While maintaining the same level of exposure, a higher frequency results in a reduced dosage on reproductive system. Further analysis reveals that, considering the public exposure threshold, the employment of higher frequency bands in 5G sub-6 GHz does not present a greater dosage on reproductive system compared to lower frequency bands. Consequently, with regard to dosage, there is no need for excessive concern among the general public regarding the impact of electromagnetic radiation emitted by 5G base stations operating below 6 GHz on male reproductive health.

Electromagnetic exposure has become increasingly widespread, and its biological effects have received extensive attention. The purpose of this study was to explore changes in the metabolism profile of the brain and serum and to identify differentially expressed proteins in the brain after exposure to the 4.9 GHz radiofrequency (RF) field. C57BL/6 mice were randomly divided into a Sham group and an RF group, which were sham-exposed and continuously exposed to a 4.9 RF field for 35 d, 1 h/d, at an average power density (PD) of 50 W/m². After exposure, untargeted metabolomics and Tandem Mass Tags (TMT) quantitative proteomics were performed. We found 104 and 153 up- and down-regulated differentially expressed metabolites (DEMs) in the RF_Brain group and RF_Serum group, and the DEMs were significantly enriched in glycerophospholipid metabolism. Moreover, 10 up-regulated and 51 down-regulated differentially expressed proteins (DEPs) were discovered in the RF group. Functional correlation analysis showed that most DEMs and DEPs showed a significant correlation. These results suggested that 4.9 GHz exposure induced disturbance of metabolism in the brain and serum, and caused deregulation of proteins in the brain.

This article focuses on the evaluation of characteristic parameters of the plate pulse forming network (PFN) considering inductive coupling. Theoretical characteristic parameters, including impedance and transmission time, were analyzed and modified based on circuit theory. Experiments, calculations, and simulations were performed on a plate PFN sample with 11 nets to test and verify the accuracy of the modifications. It is shown that the modified evaluation significantly reduces the relative error in the characteristic parameters. In addition, the modified evaluation was applied to a parallel module containing both internal and external couplings. The results demonstrate a dramatic reduction of the relative error in the characteristic parameters of the module from 16% to 1%. This modification evaluation appears to be effective in the design of plate PFN.