Insulin-like growth factor 1 (IGF-1) is a key hormone that regulates fish growth. It acts on a variety of organs and regulates multiple signaling pathways. In order to explore the specific effects of IGF-1 on fish nutrient absorption, immune system, and other functions, the present study investigated the transcriptional changes of stomachs in tilapia by IGF injection. The tilapias were divided into two groups which were injected with saline (C group) and IGF-1 (2 μg/g body weight) (I group), respectively. After three times injections, the stomachs from the tested tilapias were collected 7 days post the first injection and the transcriptomes were sequenced by Illumina HiSeqTM 2000 platform. The results showed that a total of 155 DEGs were identified between C and I groups. By gene ontology (GO) enrichment analysis, two GO terms related to absorption function were enriched including organic acid transport, and amino acid transport which contained 6 functional DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that Staphylococcus aureus infection, as well as complement and coagulation cascades pathways were enriched and contained 6 DEGs. Taken together, the present study indicated that IGF-1 injection altered gene expression related to amino acid transporting, complement and coagulation cascades which provides a promise immunopotentiation therapy by IGF-1 in digestive tract of tilapia.

Ghrelin is a growth-promoting hormone produced by the gastrointestinal tract that plays a crucial role through the ghrelin-growth hormone secretagogue receptor (GHS-R) and growth hormone/insulin-like growth factor-1 (GH/IGF-1) axes. To explore the effect of ghrelin on the transcriptomic profile of tilapia liver, the hepatic transcriptome of tilapia was sequenced for two groups, including saline-injected control (CL) and ghrelin-injected (GL; 2 μg/g body weight) tilapia. The transcriptome of livers from the two groups was sequenced using an Illumina HiSeqTM 2000 platform and yielded approximately 310.53 million raw reads. Subsequently, approximately 308.51 million clean reads were obtained from the total raw reads using in-house Perl scripts. Approximately 92.36% clean reads were mapped to the Nile tilapia genome using RSEM. Using the DESeq package, 250 differentially expressed genes (DEGs) were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed enrichment of two pathways related to RNA transcription (ribosome biogenesis in eukaryotes pathway and RNA transport pathway), with a total of 14 functional DEGs. ATP-binding and muscle contraction terms were identified as enriched using Gene Ontology (GO), yielding a total of 28 DEGs. Finally, real-time quantitative PCR (RT-qPCR) was used to confirm the accuracy of the transcriptomic results. The results of RT-qPCR were highly consistent with the RNA-seq, indicating that results of RNA-seq were valid. The differences in gene expression between the groups indicated that ghrelin-injection altered energy metabolism and RNA transcription in the tilapia liver, which provides new information to help promote the growth of tilapia.