Nutrient retention has been studied intensively in streams, but some mechanisms and processes are not yet entirely understood, especially in open-canopy streams. We evaluated PO₄³⁻, as soluble reactive P (SRP), and NH₄⁺ uptake in 2 chronically enriched Pampean streams with different macrophytic abundance. We performed short-term nutrient additions to quantify SRP and NH₄⁺ uptake metrics throughout the year, and we investigated which hydrological and biological factors influenced nutrient uptake. The results showed that SRP and NH₄⁺ uptakes were high in relation to pristine and impaired streams elsewhere, and they did not saturate despite elevated background nutrient levels. NH₄⁺ areal uptake rate fit in a 1^st-order uptake model, but an exponential model described the relationship between SRP areal uptake rate and SRP concentration. Consistent with this finding, SRP uptake velocity tended to increase linearly, and SRP uptake length decreased linearly with SRP concentration. The analysis of factors influencing uptake metrics suggested that SRP uptake mainly depended on heterotrophic demand, whereas NH₄⁺ uptake was enhanced under more autotrophic conditions. Our results showed that nutrient uptake metrics of enriched Pampean streams were similar to those observed in streams with low nutrient levels.