Preliminary evidence suggests that conjugated linoleic acid (CLA) may reduce body weight and affect body composition. The present study assessed the effect of CLA supplementation on body fat composition in overweight and obese women, while also evaluating the liver safety of CLA use. Seventy-four obese or overweight women were randomly assigned to receive 3 g/day CLA or placebo for 12 weeks. Body composition (dual-energy X-ray absorptiometry) and liver function (¹³C-methacetin breath test and serum liver enzymes) were assessed before and after the trial. Patients receiving CLA experienced a significant reduction of total body fat expressed as mass (p = 0.0007) and percentage (p = 0.0006), android adipose tissue (p = 0.0002), gynoid adipose tissue (p = 0.0028), and visceral adipose tissue (p = 4.2 × 10⁻⁹) as well as a significant increase in lean body mass to height (p = 6.1 × 10⁻¹¹) when compared to those receiving a placebo. The maximum momentary 13C recovery changes and end-point values were significantly higher in the CLA group when compared to the placebo group (p = 0.0385 and p = 0.0076, respectively). There were no significant changes in alanine aminotransferase, asparagine aminotransferase, and gamma-glutamyl transpeptidase activities between the groups. In conclusion, CLA supplementation was well tolerated and safe for the liver, which shows beneficial effects on fat composition in overweight and obese women.

Introduction. Birthweight is one of the most important factors determining neonatal well-being. From an epidemiological viewpoint, a neonatal reference chart provides a picture of the health status of a population. Global customized growth charts seem to be the most practical in multicultural settings, allowing adjustment for ethnicity. However, regional charts might be a valuable contribution to reliable growth assessment. Our study aims to establish a reference tool for growth assessment and visualize the local potential, by creating standard charts based on the data from the tertiary center with the highest number of deliveries per year in Poland. Material and Methods. We retrospectively analysed 31,353 records from the electronic database of singleton births from a five-year period from a tertiary hospital in Poznań, Poland. We excluded pre-term deliveries and high-risk pregnancies basing on well-known factors influencing fetal growth, bringing the number of records to 21,379. The data were processed separately by gender (girls n=10,312, 48.2% and boys n=11,067, 51.8%). Percentiles were calculated for each week of gestational age. Means and standard deviations were determined. Results. Standard growth charts (including 3rd, 10th, 25th, 50th, 75th, 90th and 97th percentiles) are presented. Descriptive data of population distribution are shown. Conclusions. In conclusion, obtaining standard growth charts for mature newborns has created the opportunity for a more actual and adequate assessment of the Polish neonatal population. It should allow for the implementation of new standards in future research on perinatal care.

The existing literature does not address the question of the seasonal impact on pregnancy in Central-Eastern Europe; therefore, this study was designed to investigate the seasonal variation in gestational diabetes mellitus (GDM) based on a recent Polish sample. The data of 30,205 newborns from singleton pregnancies and their mothers, including the date and gestational age of birth, neonatal sex and weight, maternal age and parity, mode of delivery, ethnicity, and a detailed list of comorbidities (including GDM), were retrospectively analysed. The prevalence of GDM was significantly (p < 0.0001) lower in spring (14.71%) than in the other seasons (16.78%). A higher incidence of GDM was observed for mothers who underwent an oral glucose tolerance test from June to August compared to those who were tested from December to February (17.34% vs. 14.75%, p < 0.0001). Similarly, there were significant differences between seasons with higher and lower insolation. The regression analysis revealed that seasonal patterns were significantly associated with the prevalence of GDM. In conclusion, this large retrospective cohort study demonstrated seasonal changes in GDM risk. The observed seasonal patterns may equally refer to mothers of babies born at term and prematurely. Further research concerning GDM risk and other seasonal and gender associations is warranted.

In a small preliminary study, phenylketonuria and poor metabolic control were suggested as risk factors for Helicobacter pylori infection in children as detected with an antigen stool test. We aimed to determine Helicobacter pylori prevalence in an adequately sized group of individuals with phenylketonuria and healthy subjects using the standard gold test (urea breath test). Further, we correlated Helicobacter pylori infection with metabolic control. The study comprised 103 individuals with phenylketonuria and 103 healthy subjects on whom a ¹³C urea breath test was performed. Blood phenylalanine levels in the preceding year were analysed. The infection rate did not differ between individuals with phenylketonuria and healthy subjects (10.7% vs 15.5%; p = 0.41). The frequency of testing and phenylalanine concentrations of Helicobacter pylori-positive and Helicobacter pylori-negative patients with phenylketonuria did not differ (p = 0.92 and p = 0.54, respectively). No associations were detected for body mass index or metabolic control. Forward stepwise regression models revealed that age (p = 0.0009–0.0016) was the only independent correlate of Helicobacter pylori infection with a relatively low fraction of the variability of the condition being explained (adjR² = 0.0721–0.0754; model p = 0.020–0.023). In conclusion, Helicobacter pylori infection in phenylketonuria is not more frequent than in the general population. Moreover, it does not depend on metabolic control.

Advanced glycation end products (AGEs) have been suggested to play an important role in osteoporosis. However, no randomised controlled trial has investigated the effect of a diet with low AGEs on densitometric parameters. This study will evaluate the effect of a 12-month low AGEs diet on bone health and cardio-metabolic parameters in postmenopausal overweight and obese women. In total, 80 subjects will be included in the study and randomly divided into two groups: low AGEs diet and standard AGEs diet for 12 months. The mineral density and content will be measured at the lumbar spine, femoral neck, and total body during the pre- and post-intervention period. Selected markers of bone formation and resorption will be assessed. Anthropometric parameters and body composition will be evaluated and markers of glucose, insulin, lipid metabolism, inflammatory and endothelial parameters will be measured. Adherence to the diet will be monitored using dietary records.

Vitamin D plays a key role in pregnancy beyond calcium–phosphate regulation, modulating immune responses and glucose metabolism via the vitamin D receptor (VDR). Placental expression may be altered in gestational diabetes mellitus (GDM). This study aimed to assess placental VDR expression in GDM and evaluate its association with maternal vitamin D levels and clinical parameters. VDR expression in the placental tissue of 53 women with GDM and 26 healthy controls was assessed semi-quantitatively by immunohistochemistry. Maternal serum 25-hydroxyvitamin D (25(OH)D) levels, body mass index, weight gain, neonatal outcomes, and other variables were evaluated. Univariate and multivariate linear regression analyses were performed. VDR expression was significantly higher in the GDM group compared to controls (p = 0.0297 for mean, p = 0.0378 for median). No significant differences were observed in serum 25(OH)D concentrations between groups. Stepwise regression revealed that diabetes was the only independent predictor of VDR expression. Within the GDM subgroup, VDR expression was not associated with any clinical parameters, including maternal vitamin D status. Upregulated placental VDR in GDM may represent an adaptive response to metabolic stress. These findings suggest complex regulation of vitamin D signalling in diabetic pregnancies, warranting further investigation.