Grass-finished beef (GFB) can provide beneficial bioactive compounds to healthy diets, including omega-3 polyunsaturated fatty acids (n-3 PUFAs), conjugated linoleic acid (CLA), and secondary bioactive compounds, such as phytochemicals. The objective of this study was to compare fatty acids (FAs), micronutrients, and phytochemicals of beef fed a biodiverse pasture (GRASS), a total mixed ration (GRAIN), or a total mixed ration with 5% grapeseed extract (GRAPE). This was a two-year study involving fifty-four Red Angus steers (n = 54). GFB contained higher levels of n-3 PUFAs, vitamin E, iron, zinc, stachydrine, hippuric acid, citric acid, and succinic acid than beef from GRAIN and GRAPE (p < 0.001 for all). No differences were observed in quantified phytochemicals between beef from GRAIN and GRAPE (p > 0.05). Random forest analysis indicated that phytochemical and FA composition of meat can predict cattle diets with a degree of certainty, especially for GFB (5.6% class error). In conclusion, these results indicate that GFB contains higher levels of potentially beneficial bioactive compounds, such as n-3 PUFAs, micronutrients, and phytochemicals, compared to grain-finished beef. Additionally, the n-6:n-3 ratio was the most crucial factor capable of separating beef based on finishing diets.

Beef raised using rotational grazing practices on biodiverse pastures offers potential benefits to animal and environmental health and can improve the nutrient density of meat to favor human health. However, many cattle producers contend with the seasonal unavailability of fresh forage, necessitating the utilization of supplementary feeds or indoor feeding. The objective of this study was to profile secondary metabolites and fatty acids in grass-finished beef supplemented with different feeds (4.5 kg/head/day) and to explore the potential for grass-finished beef authentication. In this two-year study, steers (n = 115) were randomly allocated to one of four diets: 1) pastured/supplemented with hay (control group), 2) pastured/supplemented with baleage, 3) pastured/supplemented with soybean hulls, or 4) baleage/soybean hulls in confinement. Secondary metabolites and fatty acids were measured using UHPLC-MS/MS and GC-MS, respectively. Of the 94 measured metabolites, pyridoxine, alpha-tocopherol, hippuric acid, and gallic acid differed between diets (p < 0.05 for all). Based on random forest classification, beef from the pasture/hay, pasture/baleage, pasture/soybean hulls, and confinement baleage/soybean hulls groups could be identified with a predictive accuracy of 100%, 50%, 41%, and 97%, respectively. Although minimal significant differences were observed, our data indicate that certain supplemental feeds maintain favorable nutritional profiles of grass-finished beef. In addition, metabolomics can predict cattle on exclusively forage-based or feed-based diets with a high degree of certainty.

Food waste is a barrier to the development of sustainable food systems, and a large portion of it occurs at the household level. Household food waste can be decreased by using appropriate packaging. Despite the high rate of food waste in US households, little is known about how packaging affects this. This study assessed US consumers’ awareness of how structural packaging designs and technologies affect food freshness and their willingness to purchase and to pay extra for packaging designed to reduce household food waste. To gather data, 1000 US consumers were surveyed online. Responses were analyzed overall and by population segments. The impacts of only 3 out of 15 structural packaging designs on maintaining food freshness were known by >50% of consumers. Regarding packaging technologies, while 78% of consumers knew about the impact of vacuum packaging on maintaining food freshness, just 27.6, 23, and 16% knew how modified atmosphere packaging, active packaging, and aseptic packaging affected food freshness. Only 32% of consumers knew that intelligent packaging provides information on food freshness. Just 9% of consumers recognized that foods in plastic pouches and cans possess the same food freshness. Approximately 91% of consumers will always/sometimes buy food in most of the above packaging technologies after learning about them. Half were willing to pay more for food in packages that reduce household food waste, and 40% may. Differences (p ≤ 0.05) and two-way interactions were observed between population segments. This study’s findings can help develop new packaging, education campaigns, and policies to reduce household food waste in the US.

Medical genetic studies focus on high-risk groups to enrich for targeted phenotypes. Although genetic effects may vary across the lifespan, age-related enrichment of target phenotypes can also arise from cumulative environmental exposures, without requiring changes in genetic architecture. Accumulating environmental effects over the lifetime can inflate nongenetic variance, creating the illusion of greater genetic signal while in fact masking underlying genetic effects. We propose investigating the genetic architecture of complex phenotypes in lower-risk, younger individuals, which may reduce residual variance and improve power to isolate the genetic underpinnings of complex disease-related traits. To illustrate this, we analyzed 9 complex traits across age-stratified White European cohorts in the UK Biobank. Genome-wide association analyses reveal a greater number of genome-wide significant SNPs in younger cohorts. Complementing these results, heritability analyses show a higher relative genetic contribution in younger cohorts. Together, these patterns demonstrate that genetic signals are more readily detected in lower-risk, younger individuals. This perspective suggests that temporal dynamics in genetic architecture should guide participant selection, challenging the traditional focus on high-risk older groups and highlighting the value of targeting higher-heritability groups for genetic discovery.