The burden of neoplastic diseases is a significant global health challenge accounting for thousands of deaths. In Uganda, about 32,617 cancer cases were reported in 2018, accompanied by 21,829 deaths. In a view to identify some potential anticancer plant candidates for possible drug development, the current study was designed to compile the inventory of plants with reported anticancer activity used in rural Uganda and the evidences supporting their use in cancer therapy. An electronic survey in multidisciplinary databases revealed that 29 plant species belonging to 28 genera distributed among 24 families have been reported to be used in the management of cancer in Uganda. Anticancer plants were majorly from the families Bignoniaceae (7%), Caricaceae (7%), Fabaceae (7%), Moraceae (7%), and Rutaceae (7%). Most species occur in the wild (52%), though some are cultivated (48%). The growth habit of the plants is as trees (55%) or herbs (45%). Anticancer extracts are usually prepared from leaves (29%), bark (24%), roots (21%), and fruits (13%) through decoctions (53%), as food spices (23%) or pounded to produce ointments that are applied topically (10%). Prunus africana (Hook.f.) Kalkman, Opuntia species, Albizia coriaria (Welw. ex Oliver), Daucus carota L., Cyperus alatus (Nees) F. Muell., Markhamia lutea (Benth.) K. Schum., and Oxalis corniculata L. were the most frequently encountered species. As per global reports, Allium sativum L., Annona muricata L., Carica papaya L., Moringa oleifera Lam., Opuntia species, Prunus africana (Hook.f.) Kalkman, and Catharanthus roseus (L.) G. Don. are the most studied species, with the latter having vincristine and vinblastine anticancer drugs developed from it. Prostate, cervical, breast, and skin cancers are the top traditionally treated malignancies. There is a need to isolate and evaluate the anticancer potential of the bioactive compounds in the unstudied claimed plants, such as Cyperus alatus (Nees) F. Muell., Ficus dawei Hutch., Ficus natalensis Hochst., and Lovoa trichilioides Harms, and elucidate their mechanism of anticancer activity.

Aflatoxins are endemic in Kenya. The 2004 outbreak of acute aflatoxicosis in the country was one of the unprecedented epidemics of human aflatoxin poisoning recorded in mycotoxin history. In this study, an elaborate review was performed to synthesize Kenyas major findings in relation to aflatoxins, their prevalence, detection, quantification, exposure assessment, prevention, and management in various matrices. Data retrieved indicate that the toxins are primarily biosynthesized by Aspergillus flavus and A. parasiticus, with the eastern part of the country reportedly more aflatoxin-prone. Aflatoxins have been reported in maize and maize products (Busaa, chan’gaa, githeri, irio, muthokoi, uji, and ugali), peanuts and its products, rice, cassava, sorghum, millet, yams, beers, dried fish, animal feeds, dairy and herbal products, and sometimes in tandem with other mycotoxins. The highest total aflatoxin concentration of 58,000μg/kg has been reported in maize. At least 500 acute human illnesses and 200 deaths due to aflatoxins have been reported. The causes and prevalence of aflatoxins have been grossly ascribed to poor agronomic practices, low education levels, and inadequate statutory regulation and sensitization. Low diet diversity has aggravated exposure to aflatoxins in Kenya because maize as a dietetic staple is aflatoxin-prone. Detection and surveillance are only barely adequate, though some exposure assessments have been conducted. There is a need to widen diet diversity as a measure of reducing exposure due to consumption of aflatoxin-contaminated foods.

Lake Victoria (L. Victoria) is the largest African tropical and freshwater lake, with one of the highest pollution levels, globally. It is shared among Uganda, Kenya and Tanzania, but it is drained only by the river Nile, the longest river in Africa. Though environmental studies have been conducted in the lake, investigations of the heavy metals (HMs) contamination of sediments from fish landing sites and ports on the Ugandan portion of L. Victoria are limited. In this study, sediments of an urban, industrial and fish landing site (Port Bell) on L. Victoria, Uganda was investigated to establish its HMs pollution levels and potential health risks to humans and ecosystems. Sediment samples were collected in triplicate (n = 9) from three different points of Port Bell, digested and analyzed using atomic absorption spectrometry for the presence of these HMs: copper (Cu), lead (Pb), cadmium (Cd) and chromium (Cr). The average daily dose through dermal contact and hazard quotient (HQ) were calculated to assess the health risk that is associated with dredging works (lake sand mining). Four geochemical enrichment indices: contamination factor (CF), geo-accumulation index (I_geo), pollution load index (PLI) and potential ecological risk (PERI) were used to quantify the contamination of the HMs in the sediments. The results showed that the mean HM content of the samples ranged from: 6.111 ± 0.01 to 7.111 ± 0.002 mg/kg for Cu; from 40.222 ± 0.003 to 44.212 ± 0.002 mg/kg for Pb; from 0.352 ± 0.007 to 0.522 ± 0.010 mg/kg for Cr; from 3.002 ± 0.002 to 3.453 ± 0.003 mg/kg for Cd. Health risk assessments indicated that there are no discernible non-carcinogenic health risks that could arise from the dredging works that are conducted in the study area as the indices were all below one. The contamination factors that were obtained suggest that Cd has reached a state of severe enrichment in the sediments (CF > 6). An assessment using I_geo established that the sediments were not contaminated with regards to Cu and Cr, but they exhibited low-to-median and median contamination with respect to Pb and Cd, respectively. Though the pollution load indices show that the contamination levels raise no serious concerns, the potential ecological risk indices show that there is considerable pollution of the Port Bell sediments, particularly with regard to Cd. Upon examination using multivariate statistical analyses, Cd and Cr showed a strong correlation which alluded to their introduction from anthropogenic sources. Based on the sedimentary HMs concentrations and the environmental indices that are employed in this study, it is recommended that the spatial variations in the concentrations of the HMs in water, sediments and biota should be monitored.

Aims: Aloe barbadensis Miller (A. barbadensis) is one of the most treasured species from the Aloe genus that has been used in management of various ailments. However, there are few reports on the secondary metabolites, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of its leaf latex and gel. This study aimed at comparing the phytochemicals and antioxidant activity of phosphate buffered saline (PBS) and distilled water extracts of latex and gel of A. barbadensis leaves from Kisumu, Elgeyo Marakwet and Baringo Counties of Kenya, East Africa. Study Design: The study employed quantitative and qualitative research designs. Place and Duration of Study: This study was conducted at the Department of Chemistry and Biochemistry, School of Sciences and Aerospace Studies, Moi University, Eldoret, Kenya between May 2021 and August 2021. Methodology: Leaf samples were extracted by maceration using distilled water and PBS. Phytochemical screening was performed following standard screening procures while TPC, TFC and antioxidant activity were determined using Folin-Ciocalteau method, Aluminum Chloride colorimetric assay and DPPH radical scavenging assay, respectively. One-way analysis of variance (ANOVA) was performed with Tukey post hoc test at P = .05. Correlations among TPC, TFC and antioxidant activity of the extracts were assessed using Pearson’s bivariate correlation. The analyses were performed using GraphPad Prism for windows (v9.0, GraphPad Software, California, USA). Results: Phytochemical screening results indicated the presence of alkaloids, phenols, saponins, flavonoids, cardiac glycosides, tannins, steroids, terpenes and quinones as the main secondary metabolites in the extracts. TPC and TFC were found to be highest for the aqueous extracts, with the highest contents (102.393 ± 0.121 mg GAE/ g DW and 47.228 ± 0.248 mg QE / g DW) being for dry latex of leaves from Baringo. The highest antioxidant activity (IC₅₀ = 21.900 ± 0.0594 mg/mL) was for aqueous extract of fresh latex of leaves from Baringo, followed by those from Elgeyo Marakwet and then Kisumu. Conclusion: This study established that dry and fresh latex and gel extracts of A. barbadensis leaves possess therapeutic phytochemicals with antioxidant activities, which support their use in traditional phytomedicine in Kenya.